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Genetics
Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation (Video) | JoVE
Published: May 05, 2023
doi:
10.3791/200310
Chengyin Li*
1
,
Phoebe A. W. Bhagoutie*
1
,
Victor Lao
,
Arneet L. Saltzman
1
Department of Cell and Systems Biology
,
University of Toronto
Tags
Keywords: Sonication
Formaldehyde-Crosslinked
Caenorhabditis Elegans
Chromatin Immunoprecipitation
Resuspension Buffer
Detergents
Lysate
Centrifugation
Immunoprecipitation
Anti-H3K9 Trimethylation
Anti-histone H3
IgG
Protein G
Magnetic Beads
FA-150 Buffer
Chromatin IP Elution Buffer
Reverse Cross-linking
DNA Elution
check_url/200310?article_type=t
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DOI
Cite This Article
Sonication of Formaldehyde-Crosslinked
Caenorhabditis elegans
and Chromatin Immunoprecipitation.
J. Vis. Exp.
(Pending Publication), e200310, doi:10.3791/200310 (2023).
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