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Medicine
Manufacturing Chimeric Antigen Receptor T-Cells Using an Automated Cell Processor (Video) | JoVE
Published: August 18, 2023
doi:
10.3791/200476
Rene Machietto
,
Nicholas Giacobbe
,
Jessica Perazzelli
,
Ted J. Hofmann
,
Allison Barz Leahy
3
,
Stephan A. Grupp
2,3
,
Yongping Wang
3,4
,
Stephan Kadauke
3,4
1
Cell and Gene Therapy Laboratory, Department of Pathology and Laboratory Medicine
,
Children’s Hospital of Philadelphia
,
2
Division of Oncology, Department of Pediatrics
,
Children’s Hospital of Philadelphia
,
3
Perelman School of Medicine at the University of Pennsylvania
,
4
Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine
,
Children’s Hospital of Philadelphia
Tags
Keywords: Chimeric Antigen Receptor T-Cells
Automated Cell Processor
T-cell Transduction
CD4/CD8 Selection
T-cell Enrichment
Activation Reagent
Transduction
Cell Cultivation
Vector Volume
check_url/200476?article_type=t
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DOI
Cite This Article
Kadauke, S. Manufacturing Chimeric Antigen Receptor T-Cells Using an Automated Cell Processor.
J. Vis. Exp.
(Pending Publication), e200476, doi:10.3791/200476 (2023).
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