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Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue (Video) | JoVE

Published: October 27, 2023

doi:

Guanyu Su, Wei Wang, Lingjuan Xu, Rong Liu, Yongyao Tan, Bihui Jin, Chunxiu You, Xiao Zhou, Yihong Xiong, Huatao Xie, Guigang Li

¹Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, ²Institute of Medicine Nursing,Hubei University of Medicine, ³Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

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Limbal Niche Cells LNCs Collagenase A Matrigel Basement Membrane Matrix MESCM Trypsin-EDTA Knockout Serum Cell Isolation Cell Culture Cornea Sterile Technique

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Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue. J. Vis. Exp. (Pending Publication), e200727, doi:10.3791/200727 (2023).

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