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Medicine
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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
/
عزل الخلايا المتخصصة القزحية (LNCs) من أنسجة النسيان
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JoVE Journal
Medicine
Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue
Please note that all translations are automatically generated.
Click here for the English version.
عزل الخلايا المتخصصة القزحية (LNCs) من أنسجة النسيان
A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
DOI:
10.3791/200727-v
•
05:24 min
•
October 27, 2023
•
Guanyu Su
,
Wei Wang
,
Lingjuan Xu
,
Rong Liu
,
Yongyao Tan
,
Bihui Jin
,
Chunxiu You
,
Xiao Zhou
,
Yihong Xiong
,
Huatao Xie
,
Guigang Li
1
Department of Ophthalmology, Tongji Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Institute of Medicine Nursing
,
Hubei University of Medicine
,
3
Department of Ophthalmology, Union Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
Tags
Keywords: Limbal Niche Cells
LNCs
Collagenase A
Matrigel
Basement Membrane Matrix
MESCM
Trypsin-EDTA
Knockout Serum
Cell Isolation
Cell Culture
Cornea
Sterile Technique
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