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Medicine
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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
/
Identification of Limbal Niche Cells (LNCs) (Video) | JoVE
JoVE Journal
Medicine
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JoVE Journal
Medicine
Identification of Limbal Niche Cells (LNCs)
Identification of Limbal Niche Cells (LNCs) (Video) | JoVE
A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
DOI:
10.3791/200728-v
•
03:27 min
•
October 27, 2023
•
Guanyu Su
,
Wei Wang
,
Lingjuan Xu
,
Rong Liu
,
Yongyao Tan
,
Bihui Jin
,
Chunxiu You
,
Xiao Zhou
,
Yihong Xiong
,
Huatao Xie
,
Guigang Li
1
Department of Ophthalmology, Tongji Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Institute of Medicine Nursing
,
Hubei University of Medicine
,
3
Department of Ophthalmology, Union Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
Tags
Keywords: Limbal Niche Cells (LNCs)
Immunofluorescence Staining
Trypsin EDTA
Matrigel
Knockout Serum
MESCM
Cytofuge
Paraformaldehyde
Triton X-100
Bovine Serum Albumin
PBST
DAPI
RNA Isolation
CDNA Transcription
QPCR
PanCK
VIM
CD90
CD105
SCF
PDGFR
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