A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

October 27, 2023

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue

Identification of Limbal Niche Cells (LNCs)

Guanyu Su, Wei Wang, Lingjuan Xu, Rong Liu, Yongyao Tan, Bihui Jin, Chunxiu You, Xiao Zhou, Yihong Xiong, Huatao Xie, Guigang Li

¹Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, ²Institute of Medicine Nursing,Hubei University of Medicine, ³Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

Summary

Here, we present a protocol to isolate and identify the human limbal niche cells.

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