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Bioengineering
Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation (Video) | JoVE
Published: February 02, 2024
doi:
10.3791/200881
Rosa L. Vincent
,
Fangda Li
,
Edward R. Ballister
,
Nicholas Arpaia
3
,
Tal Danino
3,4
1
Department of Biomedical Engineering
,
Columbia University
,
2
Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons
,
Columbia University
,
3
Herbert Irving Comprehensive Cancer Center
,
Columbia University
,
4
Data Science Institute
,
Columbia University
Tags
Keywords: Murine T-cells
CAR-T Cell Generation
Transfection
Transduction
Phoenix Eco Plate
Viral Supernatant
Splenocytes
CD3 Positive T-cells
Negative Selection
T-cell Activation
Human Fibronectin Transduction Enhancer
check_url/200881?article_type=t
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Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation.
J. Vis. Exp.
(Pending Publication), e200881, doi:10.3791/200881 (2024).
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