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Biology
Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research
Published: March 15, 2024
doi:
10.3791/201329
Julia K. Panzer
,
Pablo A. Garcia
,
Alberto Pugliese
1
Department of Diabetes Immunology
,
Arthur Riggs Diabetes and Metabolism Research Institute
Tags
Perifusion
Calcium Imaging
Pancreatic Disease Research
Insulin Secretion
Glucagon Detection
Agarose Embedding
Slice Chamber
Baseline Buffer
Loading Solutions
Chamber Heating
Tray Cooling
Calcium Dye
Confocal Microscopy
check_url/201329?article_type=t
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Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research.
J. Vis. Exp.
(Pending Publication), e201329, doi:10.3791/201329 (2024).
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