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Journal
/
Biology
/
Slicing Human Pancreas for Islet Function Research
/
Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research
Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research
Slicing Human Pancreas for Islet Function Research
DOI:
10.3791/201329-v
•
03:57 min
•
March 15, 2024
•
Julia K. Panzer
,
Pablo A. Garcia
,
Alberto Pugliese
1
Department of Diabetes Immunology
,
Arthur Riggs Diabetes and Metabolism Research Institute
Tags
Keywords: Perifusion
Calcium Imaging
Pancreatic Disease Research
Insulin Secretion
Glucagon Detection
Agarose Embedding
Slice Chamber
Baseline Buffer
Loading Solutions
Chamber Heating
Tray Cooling
Calcium Dye
Confocal Microscopy
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