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Biology
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
Published: January 05, 2024
doi:
10.3791/201505
Hiroko Shinjo
,
Gaku Nagano
,
Shogo Ishii
,
Natsumi Himeno
,
Yutaro Yamamoto
,
Junji Sagawa
,
Ryuta Baba
,
Gentaro Egusa
,
Noboru Hattori
,
Haruya Ohno
1
Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences
,
Hiroshima University
Tags
Keywords: Epitope Tag
Protein Precipitation
Immunoblotting
Protein-protein Interaction
Centrifugation
Protein Lysis Buffer
Protease Inhibitor
Sample Buffer
SDS-PAGE
PVDF Membrane
Primary Antibody
DYKDDDDK-PRDM16
HA-EHMT1
HEK 293 Cells
check_url/201505?article_type=t
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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies.
J. Vis. Exp.
(Pending Publication), e201505, doi:10.3791/201505 (2024).
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