JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
/
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
DOI:
10.3791/201505-v
•
02:58 min
•
January 05, 2024
•
Hiroko Shinjo
,
Gaku Nagano
,
Shogo Ishii
,
Natsumi Himeno
,
Yutaro Yamamoto
,
Junji Sagawa
,
Ryuta Baba
,
Gentaro Egusa
,
Noboru Hattori
,
Haruya Ohno
1
Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences
,
Hiroshima University
Tags
Keywords: Epitope Tag
Protein Precipitation
Immunoblotting
Protein-protein Interaction
Centrifugation
Protein Lysis Buffer
Protease Inhibitor
Sample Buffer
SDS-PAGE
PVDF Membrane
Primary Antibody
DYKDDDDK-PRDM16
HA-EHMT1
HEK 293 Cells
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/201505?article_type=v
Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation
check_url/201505?article_type=v
Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation
check_url/201505?article_type=v
Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel
check_url/201505?article_type=v
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
Read Article
