Brian Haab Van Andel Research Institute Biography Publications Institution JoVE Articles Brian Haab has not added a biography. If you are Brian Haab and would like to personalize this page please email our Author Liaison for assistance. Publications Deciphering Protein Glycosylation by Computational Integration of On-chip Profiling, Glycan-array Data, and Mass Spectrometry Molecular & Cellular Proteomics : MCP. Sep, 2018 | Pubmed ID: 30257876 Mining High-Complexity Motifs in Glycans: A New Language To Uncover the Fine Specificities of Lectins and Glycosidases Analytical Chemistry. Nov, 2017 | Pubmed ID: 29058413 The CA19-9 and Sialyl-TRA Antigens Define Separate Subpopulations of Pancreatic Cancer Cells Scientific Reports. Jun, 2017 | Pubmed ID: 28642461 A Gastric Glycoform of MUC5AC Is a Biomarker of Mucinous Cysts of the Pancreas PloS One. 2016 | Pubmed ID: 27992432 Characterizing Protein Glycosylation Through On-Chip Glycan Modification and Probing Analytical Chemistry. 12, 2016 | Pubmed ID: 27809484 Glycans Related to the CA19-9 Antigen Are Elevated in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy over CA19-9 Cellular and Molecular Gastroenterology and Hepatology. Feb, 2016 | Pubmed ID: 26998508 Multiplexed Autoantigen Microarrays Identify HLA As a Key Driver of Anti-desmoglein and -non-desmoglein Reactivities in Pemphigus Proceedings of the National Academy of Sciences of the United States of America. Feb, 2016 | Pubmed ID: 26831096 Definitive Characterization of CA 19-9 in Resectable Pancreatic Cancer Using a Reference Set of Serum and Plasma Specimens PloS One. 2015 | Pubmed ID: 26431551 Segment and Fit Thresholding: a New Method for Image Analysis Applied to Microarray and Immunofluorescence Data Analytical Chemistry. Oct, 2015 | Pubmed ID: 26339978 Upregulation of Glycans Containing 3' Fucose in a Subset of Pancreatic Cancers Uncovered Using Fusion-tagged Lectins Journal of Proteome Research. Jun, 2015 | Pubmed ID: 25938165 Exploring the Specificities of Glycan-binding Proteins Using Glycan Array Data and the GlycoSearch Software Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25753713 Glycan Motif Profiling Reveals Plasma Sialyl-lewis X Elevations in Pancreatic Cancers That Are Negative for Sialyl-lewis A Molecular & Cellular Proteomics : MCP. May, 2015 | Pubmed ID: 25733690 The Detection and Discovery of Glycan Motifs in Biological Samples Using Lectins and Antibodies: New Methods and Opportunities Advances in Cancer Research. 2015 | Pubmed ID: 25727148 Detection of Distinct Glycosylation Patterns on Human γ-glutamyl Transpeptidase 1 Using Antibody-lectin Sandwich Array (ALSA) Technology BMC Biotechnology. 2014 | Pubmed ID: 25479762 MALDI Imaging Mass Spectrometry Profiling of N-glycans in Formalin-fixed Paraffin Embedded Clinical Tissue Blocks and Tissue Microarrays PloS One. 2014 | Pubmed ID: 25184632 Mesenchymal-like Pancreatic Cancer Cells Harbor Specific Genomic Alterations More Frequently Than Their Epithelial-like Counterparts Molecular Oncology. Oct, 2014 | Pubmed ID: 24837184 Array-based Immunoassays with Rolling-circle Amplification Detection Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24623215 Characterization of Glycoproteins in Pancreatic Cyst Fluid Using a High-performance Multiple Lectin Affinity Chromatography Platform Journal of Proteome Research. Jan, 2014 | Pubmed ID: 24303806 Using Antibody Arrays to Measure Protein Abundance and Glycosylation: Considerations for Optimal Performance Current Protocols in Protein Science. Sep, 2013 | Pubmed ID: 24510592 Prediction of Glycan Motifs Using Quantitative Analysis of Multi-lectin Binding: Motifs on MUC1 Produced by Cultured Pancreatic Cancer Cells Proteomics. Clinical Applications. Oct, 2013 | Pubmed ID: 23956151 Determining Lectin Specificity from Glycan Array Data Using Motif Segregation and GlycoSearch Software Current Protocols in Chemical Biology. 2013 | Pubmed ID: 23839995 Specific Glycoforms of MUC5AC and Endorepellin Accurately Distinguish Mucinous from Nonmucinous Pancreatic Cysts Molecular & Cellular Proteomics : MCP. Oct, 2013 | Pubmed ID: 23836919 Modulation of Glycan Detection on Specific Glycoproteins by Lectin Multimerization Analytical Chemistry. Feb, 2013 | Pubmed ID: 23286506 Using Lectins in Biomarker Research: Addressing the Limitations of Sensitivity and Availability Proteomics. Clinical Applications. Aug, 2012 | Pubmed ID: 22927350 Diverse Monoclonal Antibodies Against the CA 19-9 Antigen Show Variation in Binding Specificity with Consequences for Clinical Interpretation Proteomics. Jul, 2012 | Pubmed ID: 22623153 Comparison of Surgical and Endoscopic Sample Collection for Pancreatic Cyst Fluid Biomarker Identification Journal of Proteome Research. Apr, 2012 | Pubmed ID: 22439797 The Fine Specificity of Mannose-binding and Galactose-binding Lectins Revealed Using Outlier Motif Analysis of Glycan Array Data Glycobiology. Jan, 2012 | Pubmed ID: 21875884 PloS One. 2011 | Pubmed ID: 22220206 High-throughput Studies of Protein Glycoforms Using Antibody-lectin Sandwich Arrays Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21901603 Identification of Blood-protein Carriers of the CA 19-9 Antigen and Characterization of Prevalence in Pancreatic Diseases Proteomics. Sep, 2011 | Pubmed ID: 21751362 Glycogene Expression Alterations Associated with Pancreatic Cancer Epithelial-mesenchymal Transition in Complementary Model Systems PloS One. 2010 | Pubmed ID: 20885998 Glycosylation Variants of Mucins and CEACAMs As Candidate Biomarkers for the Diagnosis of Pancreatic Cystic Neoplasms Annals of Surgery. May, 2010 | Pubmed ID: 20395854 Antibody-lectin Sandwich Arrays for Biomarker and Glycobiology Studies Expert Review of Proteomics. Feb, 2010 | Pubmed ID: 20121469 Antibody-array Interaction Mapping, a New Method to Detect Protein Complexes Applied to the Discovery and Study of Serum Amyloid P Interactions with Kininogen in Human Plasma Molecular & Cellular Proteomics : MCP. Mar, 2010 | Pubmed ID: 20023212 A Motif-based Analysis of Glycan Array Data to Determine the Specificities of Glycan-binding Proteins Glycobiology. Mar, 2010 | Pubmed ID: 19946132 The Development of an Integrated Platform to Identify Breast Cancer Glycoproteome Changes in Human Serum Journal of Chromatography. A. May, 2010 | Pubmed ID: 19782370 Proteomics. Clinical Applications. Aug, 2009 | Pubmed ID: 21136996 Mucin Glycosylation is Altered by Pro-inflammatory Signaling in Pancreatic-cancer Cells Journal of Proteome Research. Apr, 2009 | Pubmed ID: 19714813 Microarrays in Glycoproteomics Research Clinics in Laboratory Medicine. Mar, 2009 | Pubmed ID: 19389548 Analysis of Glycans on Serum Proteins Using Antibody Microarrays Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 19381946 The Prevalence and Nature of Glycan Alterations on Specific Proteins in Pancreatic Cancer Patients Revealed Using Antibody-lectin Sandwich Arrays Molecular & Cellular Proteomics : MCP. Jul, 2009 | Pubmed ID: 19377061 Comprehensive Proteome Analysis of an Apc Mouse Model Uncovers Proteins Associated with Intestinal Tumorigenesis Cancer Prevention Research (Philadelphia, Pa.). Mar, 2009 | Pubmed ID: 19240248 An Experimental Strategy for Quantitative Analysis of the Humoral Immune Response to Prostate Cancer Antigens Using Natural Protein Microarrays Proteomics. Clinical Applications. May, 2007 | Pubmed ID: 21136701 Low-volume, High-throughput Sandwich Immunoassays for Profiling Plasma Proteins in Mice: Identification of Early-stage Systemic Inflammation in a Mouse Model of Intestinal Cancer Molecular Oncology. Sep, 2007 | Pubmed ID: 19305640 A Hierarchical Approach Employing Metabolic and Gene Expression Profiles to Identify the Pathways That Confer Cytotoxicity in HepG2 Cells BMC Systems Biology. 2007 | Pubmed ID: 17498300 Multiplexed Analysis of Glycan Variation on Native Proteins Captured by Antibody Microarrays Nature Methods. May, 2007 | Pubmed ID: 17417647 Antibody Array Profiling Reveals Serum TSP-1 As a Marker to Distinguish Benign from Malignant Prostatic Disease The Prostate. Feb, 2007 | Pubmed ID: 17192876 Journal of Proteome Research. Aug, 2006 | Pubmed ID: 16889408 A Reagent Resource to Identify Proteins and Peptides of Interest for the Cancer Community: a Workshop Report Molecular & Cellular Proteomics : MCP. Oct, 2006 | Pubmed ID: 16867976 Applications of Antibody Array Platforms Current Opinion in Biotechnology. Aug, 2006 | Pubmed ID: 16837184 RCA-enhanced Protein Detection Arrays Methods in Molecular Biology (Clifton, N.J.). 2006 | Pubmed ID: 16785639 Profiling Bladder Cancer Using Targeted Antibody Arrays The American Journal of Pathology. Jan, 2006 | Pubmed ID: 16400012 Antibody Microarray Profiling Reveals Individual and Combined Serum Proteins Associated with Pancreatic Cancer Cancer Research. Dec, 2005 | Pubmed ID: 16322270 Multiplexed Protein Analysis Using Antibody Microarrays and Label-based Detection Methods in Molecular Medicine. 2005 | Pubmed ID: 16156104 Distinctive Serum Protein Profiles Involving Abundant Proteins in Lung Cancer Patients Based Upon Antibody Microarray Analysis BMC Cancer. 2005 | Pubmed ID: 16117833 Overview of the HUPO Plasma Proteome Project: Results from the Pilot Phase with 35 Collaborating Laboratories and Multiple Analytical Groups, Generating a Core Dataset of 3020 Proteins and a Publicly-available Database Proteomics. Aug, 2005 | Pubmed ID: 16104056 Proteomics. Aug, 2005 | Pubmed ID: 16052621 Immunoassay and Antibody Microarray Analysis of the HUPO Plasma Proteome Project Reference Specimens: Systematic Variation Between Sample Types and Calibration of Mass Spectrometry Data Proteomics. Aug, 2005 | Pubmed ID: 16038022 Optimized Normalization for Antibody Microarrays and Application to Serum-protein Profiling Molecular & Cellular Proteomics : MCP. Jun, 2005 | Pubmed ID: 15793073 Antibody Arrays in Cancer Research Molecular & Cellular Proteomics : MCP. Apr, 2005 | Pubmed ID: 15671041 Toxicology Letters. Nov, 2004 | Pubmed ID: 15454310 Haematologica. Sep, 2004 | Pubmed ID: 15377468 Cancer Research. Sep, 2004 | Pubmed ID: 15342387 Journal of Proteome Research. Mar-Apr, 2004 | Pubmed ID: 15113102 Two-color, Rolling-circle Amplification on Antibody Microarrays for Sensitive, Multiplexed Serum-protein Measurements Genome Biology. 2004 | Pubmed ID: 15059261 Multiplexed Protein Analysis Using Spotted Antibody Microarrays Methods in Molecular Biology (Clifton, N.J.). 2004 | Pubmed ID: 15020778 Microarrays of Tumor Cell Derived Proteins Uncover a Distinct Pattern of Prostate Cancer Serum Immunoreactivity Proteomics. Nov, 2003 | Pubmed ID: 14595819 Methods and Applications of Antibody Microarrays in Cancer Research Proteomics. Nov, 2003 | Pubmed ID: 14595810 Antibody Microarray Profiling of Human Prostate Cancer Sera: Antibody Screening and Identification of Potential Biomarkers Proteomics. Jan, 2003 | Pubmed ID: 12548634 Identifying Cancer-related Genes in Nasopharyngeal Carcinoma Cell Lines Using DNA and MRNA Expression Profiling Analyses International Journal of Oncology. Dec, 2002 | Pubmed ID: 12429968 Nature Medicine. Mar, 2002 | Pubmed ID: 11875502 CIT: Identification of Differentially Expressed Clusters of Genes from Microarray Data Bioinformatics (Oxford, England). Jan, 2002 | Pubmed ID: 11836234 Gene Expression Profiling of Clear Cell Renal Cell Carcinoma: Gene Identification and Prognostic Classification Proceedings of the National Academy of Sciences of the United States of America. Aug, 2001 | Pubmed ID: 11493696 Protein Microarrays for Highly Parallel Detection and Quantitation of Specific Proteins and Antibodies in Complex Solutions Genome Biology. 2001 | Pubmed ID: 11182887 Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples Chen Lu1, Joshua L. Wonsidler1, Jianwei Li2, Yanming Du1, Timothy Block3, Brian Haab4, Songming Chen1 1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute JoVE 3791 Biology
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples Chen Lu1, Joshua L. Wonsidler1, Jianwei Li2, Yanming Du1, Timothy Block3, Brian Haab4, Songming Chen1 1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute JoVE 3791 Biology