CHRIS XU School of Applied and Engineering Physics CORNELL UNIVERSITY Biography Publications Institution JoVE Articles CHRIS XU Chris Xu is the IBM Chair Professor of Engineering and director of the School of Applied and Engineering Physics, Cornell University. He is the Mong Family Foundation Director of Cornell Neurotech – Engineering. His current research areas are fiber optics and biomedical imaging, with major thrusts in multiphoton microscopy for deep brain imaging, multiphoton microendoscopy for clinical applications, and fiber-based devices and systems for telecommunications and optical imaging. Dr. Xu pioneered the development of temporal focusing and long wavelength two- and three-photon microscopy for deep tissue imaging. Prior to Cornell, he was a member of technical staff at Bell Laboratories, and pioneered breakthrough development of fiber optic communication systems based on differential phase-shift keying. He received his Ph.D. in Applied Physics, Cornell University, and his B.S. in Physics from Fudan University.Dr. Xu has chaired or served on many conference organization committees and NSF/NIH review panels. He served on the NIH NEI External Scientific Oversight Committee of the Audacious Goal Initiative and is currently serving on the OSA Biomedical Congress Strategic Planning Committee. He served as Associate Editor for Biomedical Optics Express, and is on the editorial boards of several journals. He holds 25 patents on optical communications and imaging. He has won the NSF CAREER award, Bell Labs team research award, and the Tau Beta Pi and two other teaching awards from Cornell Engineering College. He received Cornell Engineering Research Excellence Award in 2017. He is a fellow of the National Academy of Inventors and a fellow of the Optical Society of America. Publications Shot Noise Limits on Binary Detection in Multiphoton Imaging Biomedical Optics Express. Nov, 2021 | Pubmed ID: 34858697 Closed-loop Wavefront Sensing and Correction in the Mouse Brain with Computed Optical Coherence Microscopy Biomedical Optics Express. Aug, 2021 | Pubmed ID: 34513234 Multicolor Three-photon Fluorescence Imaging with Single-wavelength Excitation Deep in Mouse Brain Science Advances. Mar, 2021 | Pubmed ID: 33731355 Memorial Viewpoint for Watt W. Webb: An Experimentalist's Experimentalist The Journal of Physical Chemistry. B. 03, 2021 | Pubmed ID: 33622036 Fabrication of Injectable Micro-Scale Opto-Electronically Transduced Electrodes (MOTEs) for Physiological Monitoring Journal of Microelectromechanical Systems : a Joint IEEE and ASME Publication on Microstructures, Microactuators, Microsensors, and Microsystems. Oct, 2020 | Pubmed ID: 33071528 Deep Three-photon Imaging of the Brain in Intact Adult Zebrafish Nature Methods. 06, 2020 | Pubmed ID: 32341543 Microscopic Sensors Using Optical Wireless Integrated Circuits Proceedings of the National Academy of Sciences of the United States of America. 04, 2020 | Pubmed ID: 32303653 GHz Ultrasonic Chip-Scale Device Induces Ion Channel Stimulation in Human Neural Cells Scientific Reports. 02, 2020 | Pubmed ID: 32080204 Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain ELife. 01, 2020 | Pubmed ID: 31999253 An Adaptive Excitation Source for High-speed Multiphoton Microscopy Nature Methods. 02, 2020 | Pubmed ID: 31792434 Corrigendum to 'The Role of Systematic and Targeted Biopsies in Light of Overlap on Magnetic Resonance Imaging Ultrasound Fusion Biopsy' [European Urology Oncology 1 (2018) 263-267] European Urology Oncology. Jun, 2020 | Pubmed ID: 31378664 Impact of the Emission Wavelengths on Multiphoton Imaging of Mouse Brains Biomedical Optics Express. Apr, 2019 | Pubmed ID: 31061766 Real-time in Vivo Optical Biopsy Using Confocal Laser Endomicroscopy to Evaluate Distal Margin in Situ and Determine Surgical Procedure in Low Rectal Cancer Surgical Endoscopy. 07, 2019 | Pubmed ID: 30386986 Dynamic Visualization of the Recovery of Mouse Hepatobiliary Metabolism to Acetaminophen-overdose Damage Journal of Biophotonics. 03, 2019 | Pubmed ID: 30302934 Multiphoton Imaging Provides a Superior Optical Biopsy to That of Confocal Laser Endomicroscopy Imaging for Colorectal Lesions Endoscopy. 02, 2019 | Pubmed ID: 29996151 The Role of Systematic and Targeted Biopsies in Light of Overlap on Magnetic Resonance Imaging Ultrasound Fusion Biopsy European Urology Oncology. 09, 2018 | Pubmed ID: 31100246 Label-free Confocal Imaging of the Deep Mouse Brain with Long-wavelength Illumination Biomedical Optics Express. Dec, 2018 | Pubmed ID: 31065448 Multi-color Background-free Coherent Anti-Stokes Raman Scattering Microscopy Using a Time-lens Source Optics Express. Dec, 2018 | Pubmed ID: 30650870 Comparing the Effective Attenuation Lengths for Long Wavelength Imaging of the Mouse Brain Biomedical Optics Express. Aug, 2018 | Pubmed ID: 30338138 Three-photon Imaging of Mouse Brain Structure and Function Through the Intact Skull Nature Methods. 10, 2018 | Pubmed ID: 30202059 Investigation of the Long Wavelength Limit of Soliton Self-frequency Shift in a Silica Fiber Optics Express. Jul, 2018 | Pubmed ID: 30114134 Fiber-based Tunable Repetition Rate Source for Deep Tissue Two-photon Fluorescence Microscopy Biomedical Optics Express. May, 2018 | Pubmed ID: 29760989 Rapid Volumetric Imaging with Bessel-Beam Three-photon Microscopy Biomedical Optics Express. Apr, 2018 | Pubmed ID: 29675334 Multiple Regions of Interest on Multiparametric Magnetic Resonance Imaging Are Not Associated with Increased Detection of Clinically Significant Prostate Cancer on Fusion Biopsy The Journal of Urology. 09, 2018 | Pubmed ID: 29518433 Correcting the Limited View in Optical-resolution Photoacoustic Microscopy Journal of Biophotonics. 02, 2018 | Pubmed ID: 28971578 Two-photon Shack-Hartmann Wavefront Sensor Optics Letters. Mar, 2017 | Pubmed ID: 28295068 In Vivo Three-photon Imaging of Activity of GCaMP6-labeled Neurons Deep in Intact Mouse Brain Nature Methods. Apr, 2017 | Pubmed ID: 28218900 Characterization and Adaptive Compression of a Multi-soliton Laser Source Optics Express. Jan, 2017 | Pubmed ID: 28085826 Nonresonant Background Suppression for Coherent Anti-Stokes Raman Scattering Microscopy Using a Multi-wavelength Time-lens Source Optics Express. Nov, 2016 | Pubmed ID: 27857399 Direct Visualization of Functional Heterogeneity in Hepatobiliary Metabolism Using 6-CFDA As Model Compound Biomedical Optics Express. Sep, 2016 | Pubmed ID: 27699121 Erythrocytes Are Oxygen-Sensing Regulators of the Cerebral Microcirculation Neuron. Aug, 2016 | Pubmed ID: 27499087 Adaptive Optics in Multiphoton Microscopy: Comparison of Two, Three and Four Photon Fluorescence Optics Express. Nov, 2015 | Pubmed ID: 26698772 Dispersion Compensation in Three-photon Fluorescence Microscopy at 1,700 Nm Biomedical Optics Express. Apr, 2015 | Pubmed ID: 25909022 Femtosecond Laser Bone Ablation with a High Repetition Rate Fiber Laser Source Biomedical Optics Express. Jan, 2015 | Pubmed ID: 25657872 Multiphoton Gradient Index Endoscopy for Evaluation of Diseased Human Prostatic Tissue Ex Vivo Journal of Biomedical Optics. Nov, 2014 | Pubmed ID: 25415446 Measurements of Multiphoton Action Cross Sections for Multiphoton Microscopy Biomedical Optics Express. Oct, 2014 | Pubmed ID: 25360361 Nonlinear Structured-illumination Enhanced Temporal Focusing Multiphoton Excitation Microscopy with a Digital Micromirror Device Biomedical Optics Express. Aug, 2014 | Pubmed ID: 25136483 Wavefront Sensorless Adaptive Optics Temporal Focusing-based Multiphoton Microscopy Biomedical Optics Express. Jun, 2014 | Pubmed ID: 24940539 In Vivo Three-photon Microscopy of Subcortical Structures Within an Intact Mouse Brain Nature Photonics. Mar, 2013 | Pubmed ID: 24353743 Two-photon Fluorescence Imaging of Intracellular Hydrogen Peroxide with Chemoselective Fluorescent Probes Journal of Biomedical Optics. Oct, 2013 | Pubmed ID: 24084856 Dual Modality Endomicroscope with Optical Zoom Capability Biomedical Optics Express. Month, 2013 | Pubmed ID: 24049671 Multiphoton Microscopy to Identify and Characterize the Transition Zone in a Mouse Model of Hirschsprung Disease Journal of Pediatric Surgery. Jun, 2013 | Pubmed ID: 23845620 Time-lens Based Hyperspectral Stimulated Raman Scattering Imaging and Quantitative Spectral Analysis Journal of Biophotonics. Oct, 2013 | Pubmed ID: 23840041 Three-photon Excited Fluorescence Imaging of Unstained Tissue Using a GRIN Lens Endoscope Biomedical Optics Express. May, 2013 | Pubmed ID: 23667782 Nanotools for Neuroscience and Brain Activity Mapping ACS Nano. Mar, 2013 | Pubmed ID: 23514423 Frequency Multiplexed Multiphoton Phosphorescence Lifetime Microscopy Nature Photonics. Jan, 2013 | Pubmed ID: 23472061 Intermodal Four-wave Mixing in a Higher-order-mode Fiber Applied Physics Letters. Oct, 2012 | Pubmed ID: 23152639 Intermodal Čerenkov Radiation in a Higher-order-mode Fiber Optics Letters. Nov, 2012 | Pubmed ID: 23114312 Three-color Femtosecond Source for Simultaneous Excitation of Three Fluorescent Proteins in Two-photon Fluorescence Microscopy Biomedical Optics Express. Sep, 2012 | Pubmed ID: 23024893 Multiphoton Tomographic Imaging: a Potential Optical Biopsy Tool for Detecting Gastrointestinal Inflammation and Neoplasia Cancer Prevention Research (Philadelphia, Pa.). Nov, 2012 | Pubmed ID: 22961775 In Vivo Imaging of Unstained Tissues Using Long Gradient Index Lens Multiphoton Endoscopic Systems Biomedical Optics Express. May, 2012 | Pubmed ID: 22567597 In Vivo Imaging of Unstained Tissues Using a Compact and Flexible Multiphoton Microendoscope Journal of Biomedical Optics. Apr, 2012 | Pubmed ID: 22559671 Multifocal Multiphoton Endoscope Optics Letters. Apr, 2012 | Pubmed ID: 22513682 Fiber Delivered Two-color Picosecond Source Through Nonlinear Spectral Transformation for Coherent Raman Scattering Imaging Applied Physics Letters. Feb, 2012 | Pubmed ID: 22393265 Use of a Lensed Fiber for a Large-field-of-view, High-resolution, Fiber-scanning Microendoscope Optics Letters. Mar, 2012 | Pubmed ID: 22378425 Time-domain Multimode Dispersion Measurement in a Higher-order-mode Fiber Optics Letters. Feb, 2012 | Pubmed ID: 22297348 Fiber-delivered Picosecond Source for Coherent Raman Scattering Imaging Optics Letters. Nov, 2011 | Pubmed ID: 22048375 In Vivo Two-photon Microscopy to 1.6-mm Depth in Mouse Cortex Journal of Biomedical Optics. Oct, 2011 | Pubmed ID: 22029361 Compact and Flexible Raster Scanning Multiphoton Endoscope Capable of Imaging Unstained Tissue Proceedings of the National Academy of Sciences of the United States of America. Oct, 2011 | Pubmed ID: 22006303 High Sensitivity Third-order Autocorrelation Measurement by Intensity Modulation and Third Harmonic Detection Optics Letters. Jun, 2011 | Pubmed ID: 21686024 Generation of Cerenkov Radiation at 850 Nm in Higher-order-mode Fiber Optics Express. Apr, 2011 | Pubmed ID: 21643129 Multiphoton Microscopy for Structure Identification in Human Prostate and Periprostatic Tissue: Implications in Prostate Cancer Surgery BJU International. Nov, 2011 | Pubmed ID: 21443651 Wavelength-tunable High-energy Soliton Pulse Generation from a Large-mode-area Fiber Pumped by a Time-lens Source Optics Letters. Mar, 2011 | Pubmed ID: 21403736 High Speed Multiphoton Axial Scanning Through an Optical Fiber in a Remotely Scanned Temporal Focusing Setup Biomedical Optics Express. Dec, 2010 | Pubmed ID: 21326638 Synchronized Time-lens Source for Coherent Raman Scattering Microscopy Optics Express. Nov, 2010 | Pubmed ID: 21164749 Endoscope Lens with Dual Fields of View and Resolutions for Multiphoton Imaging Optics Letters. Aug, 2010 | Pubmed ID: 20717440 Ultralong Continuously Tunable Parametric Delays Via a Cascading Discrete Stage Optics Express. Jan, 2010 | Pubmed ID: 20173853 Deep Tissue Multiphoton Microscopy Using Longer Wavelength Excitation Optics Express. Aug, 2009 | Pubmed ID: 19654740 Enhanced Axial Confinement of Sum-frequency Generation in a Temporal Focusing Setup Optics Letters. Jun, 2009 | Pubmed ID: 19529703 1 Micros Tunable Delay Using Parametric Mixing and Optical Phase Conjugation in Si Waveguides Optics Express. Apr, 2009 | Pubmed ID: 19399074 Tunable Dispersion Compensation by a Rotating Cylindrical Lens Optics Letters. Apr, 2009 | Pubmed ID: 19370115 Generation of High Repetition Rate Femtosecond Pulses from a CW Laser by a Time-lens Loop Optics Express. Apr, 2009 | Pubmed ID: 19365484 Soliton Self-Frequency Shift: Experimental Demonstrations and Applications IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society. Month, 2008 | Pubmed ID: 23055656 Experimental and Theoretical Analysis of Core-to-core Coupling on Fiber Bundle Imaging Optics Express. Dec, 2008 | Pubmed ID: 19104591 Large Tunable Delays Using Parametric Mixing and Phase Conjugation in Si Nanowaveguides Optics Express. Jul, 2008 | Pubmed ID: 18607445 Generation of 3.5 NJ Femtosecond Pulses from a Continuous-wave Laser Without Mode Locking Optics Letters. Jun, 2007 | Pubmed ID: 17546137 Generation of Femtosecond Pulses at 1350 Nm by Cerenkov Radiation in Higher-order-mode Fiber Optics Letters. May, 2007 | Pubmed ID: 17410232 Demonstration of Soliton Self-frequency Shift Below 1300 Nm in Higher-order Mode, Solid Silica-based Fiber Optics Letters. Feb, 2007 | Pubmed ID: 17356646 Compensation of Self-phase Modulation in Fiber-based Chirped-pulse Amplification Systems Optics Letters. Jun, 2006 | Pubmed ID: 16688285 Ultrafast Optical Delay Line by Use of a Time-prism Pair Optics Letters. Jan, 2005 | Pubmed ID: 15648651 Multiwavelength Pulse Generator Using Time-lens Compression Optics Letters. Jul, 2004 | Pubmed ID: 15259716 Photonic Analog-to-digital Converter Using Soliton Self-frequency Shift and Interleaving Spectral Filters Optics Letters. Jun, 2003 | Pubmed ID: 12836754 Microscopie à fluorescence à trois photons des tissus profonds dans le cerveau intact d’une souris et d’un poisson-zèbre Yusaku Hontani*1, Najva Akbari*1, Kristine E. Kolkman2, Chunyan Wu1,3, Fei Xia1,4, Kibaek Choe1, Grace Zhang Wang1, Mariya Sokolova1, Joseph R. Fetcho2, Chris Xu1 1School of Applied and Engineering Physics, Cornell University, 2Department of Neurobiology and Behavior, Cornell University, 3College of Veterinary Medicine, Cornell University, 4Meinig School of Biomedical Engineering, Cornell University JoVE 63213 Neuroscience
Microscopie à fluorescence à trois photons des tissus profonds dans le cerveau intact d’une souris et d’un poisson-zèbre Yusaku Hontani*1, Najva Akbari*1, Kristine E. Kolkman2, Chunyan Wu1,3, Fei Xia1,4, Kibaek Choe1, Grace Zhang Wang1, Mariya Sokolova1, Joseph R. Fetcho2, Chris Xu1 1School of Applied and Engineering Physics, Cornell University, 2Department of Neurobiology and Behavior, Cornell University, 3College of Veterinary Medicine, Cornell University, 4Meinig School of Biomedical Engineering, Cornell University JoVE 63213 Neuroscience