David L. Spector Cold Spring Harbor Laboratory Cold Spring Harbor Biography Publications Institution JoVE Articles David L. Spector has not added a biography. If you are David L. Spector and would like to personalize this page please email our Author Liaison for assistance. Publications Single Oligonucleotide Capture of RNA And Temperature Elution Series ( ) for Identification of RNA-binding Proteins Bio-protocol. Dec, 2022 | Pubmed ID: 36618090 Testing for Phylogenetic Signal in Single-Cell RNA-Seq Data Journal of Computational Biology : a Journal of Computational Molecular Cell Biology. Dec, 2022 | Pubmed ID: 36475926 Platr4 is an Early Embryonic LncRNA That Exerts Its Function Downstream on Cardiogenic Mesodermal Lineage Commitment Developmental Cell. Nov, 2022 | Pubmed ID: 36347239 Simultaneous Visualization of RNA Transcripts and Proteins in Whole-mount Mouse Preimplantation Embryos Using Single-molecule Fluorescence Hybridization and Immunofluorescence Microscopy Frontiers in Cell and Developmental Biology. 2022 | Pubmed ID: 36268512 Allele-specific Differential Regulation of Monoallelically Expressed Autosomal Genes in the Cardiac Lineage Nature Communications. Oct, 2022 | Pubmed ID: 36216821 Patient-Derived Triple-Negative Breast Cancer Organoids Provide Robust Model Systems That Recapitulate Tumor Intrinsic Characteristics Cancer Research. Apr, 2022 | Pubmed ID: 35180770 Noncoding RNAs: Biology and Applications-a Keystone Symposia Report Annals of the New York Academy of Sciences. 12, 2021 | Pubmed ID: 34791665 Activating a Collaborative Innate-adaptive Immune Response to Control Metastasis Cancer Cell. 10, 2021 | Pubmed ID: 34478639 LncRNA Regulates Myc Translation in Hepatocellular Carcinoma Via Sequestering TIAR ELife. May, 2021 | Pubmed ID: 34002693 : a Long Non-coding RNA Involved in Breast Cancer Progression Molecular & Cellular Oncology. 2021 | Pubmed ID: 33860086 MaTAR25 LncRNA Regulates the Tensin1 Gene to Impact Breast Cancer Progression Nature Communications. Dec, 2020 | Pubmed ID: 33353933 Comprehensive Analysis of Structural Variants in Breast Cancer Genomes Using Single-molecule Sequencing Genome Research. Sep, 2020 | Pubmed ID: 32887686 Long Non-Coding RNA: Functional Implications Non-coding RNA. Jun, 2020 | Pubmed ID: 32503170 MALAT1 Long Non-coding RNA and Breast Cancer RNA Biology. Jun, 2019 | Pubmed ID: 30874469 POU2F3 is a Master Regulator of a Tuft Cell-like Variant of Small Cell Lung Cancer Genes & Development. 07, 2018 | Pubmed ID: 29945888 Therapeutic Targeting of Long Non-Coding RNAs in Cancer Trends in Molecular Medicine. Mar, 2018 | Pubmed ID: 29449148 Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids Bio-protocol. Aug, 2017 | Pubmed ID: 28932764 A LncRNA Fine Tunes the Dynamics of a Cell State Transition Involving , and DNA Methylation ELife. Aug, 2017 | Pubmed ID: 28820723 Identification and Characterization of a Class of MALAT1-like Genomic Loci Cell Reports. May, 2017 | Pubmed ID: 28538188 Mammary Tumor-Associated RNAs Impact Tumor Cell Proliferation, Invasion, and Migration Cell Reports. Sep, 2016 | Pubmed ID: 27681436 Differentiation of Mammary Tumors and Reduction in Metastasis Upon Malat1 LncRNA Loss Genes & Development. Jan, 2016 | Pubmed ID: 26701265 Quantitative Analysis of Chromatin Interaction Changes Upon a 4.3 Mb Deletion at Mouse 4E2 BMC Genomics. Nov, 2015 | Pubmed ID: 26589460 Regulation of the ESC Transcriptome by Nuclear Long Noncoding RNAs Genome Research. Sep, 2015 | Pubmed ID: 26048247 Transient Pairing of Homologous Oct4 Alleles Accompanies the Onset of Embryonic Stem Cell Differentiation Cell Stem Cell. Mar, 2015 | Pubmed ID: 25748933 Non-rigid Multi-frame Registration of Cell Nuclei in Live Cell Fluorescence Microscopy Image Data Medical Image Analysis. Jan, 2015 | Pubmed ID: 25181702 Random Monoallelic Expression: Regulating Gene Expression One Allele at a Time Trends in Genetics : TIG. Jun, 2014 | Pubmed ID: 24780084 Identification and Initial Functional Characterization of a Human Vascular Cell-enriched Long Noncoding RNA Arteriosclerosis, Thrombosis, and Vascular Biology. Jun, 2014 | Pubmed ID: 24578380 Random Monoallelic Gene Expression Increases Upon Embryonic Stem Cell Differentiation Developmental Cell. Feb, 2014 | Pubmed ID: 24576421 Long Non-coding RNAs: Modulators of Nuclear Structure and Function Current Opinion in Cell Biology. Feb, 2014 | Pubmed ID: 24529241 Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements Stem Cell Reports. 2013 | Pubmed ID: 24286025 Role of SWI/SNF in Acute Leukemia Maintenance and Enhancer-mediated Myc Regulation Genes & Development. Dec, 2013 | Pubmed ID: 24285714 Receptor-mediated Delivery of Engineered Nucleases for Genome Modification Nucleic Acids Research. Oct, 2013 | Pubmed ID: 23956220 Chromatin Meets Its Organizers Cell. Jun, 2013 | Pubmed ID: 23746835 Lamin A/C is Expressed in Pluripotent Mouse Embryonic Stem Cells Nucleus (Austin, Tex.). 2013 | Pubmed ID: 23324457 Chromatin Organization and Transcriptional Regulation Current Opinion in Genetics & Development. Apr, 2013 | Pubmed ID: 23270812 The Noncoding RNA MALAT1 is a Critical Regulator of the Metastasis Phenotype of Lung Cancer Cells Cancer Research. Feb, 2013 | Pubmed ID: 23243023 The LncRNA Malat1 is Dispensable for Mouse Development but Its Transcription Plays a Cis-regulatory Role in the Adult Cell Reports. Jul, 2012 | Pubmed ID: 22840402 Gene Bookmarking Accelerates the Kinetics of Post-mitotic Transcriptional Re-activation Nature Cell Biology. Oct, 2011 | Pubmed ID: 21983563 Biogenesis and Function of Nuclear Bodies Trends in Genetics : TIG. Aug, 2011 | Pubmed ID: 21680045 Four Amino Acids Guide the Assembly or Disassembly of Arabidopsis Histone H3.3-containing Nucleosomes Proceedings of the National Academy of Sciences of the United States of America. Jun, 2011 | Pubmed ID: 21670303 Direct Visualization of the Co-transcriptional Assembly of a Nuclear Body by Noncoding RNAs Nature Cell Biology. Jan, 2011 | Pubmed ID: 21170033 Nuclear Speckles Cold Spring Harbor Perspectives in Biology. Feb, 2011 | Pubmed ID: 20926517 Nonrigid Registration of 2-D and 3-D Dynamic Cell Nuclei Images for Improved Classification of Subcellular Particle Motion IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society. Apr, 2011 | Pubmed ID: 20840894 Regulation of the Histone H4 Monomethylase PR-Set7 by CRL4(Cdt2)-mediated PCNA-dependent Degradation During DNA Damage Molecular Cell. Nov, 2010 | Pubmed ID: 21035370 Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression Molecular Biology of the Cell. Dec, 2010 | Pubmed ID: 20881057 A Long Nuclear-retained Non-coding RNA Regulates Synaptogenesis by Modulating Gene Expression The EMBO Journal. Sep, 2010 | Pubmed ID: 20729808 Chromatin Dynamics Annual Review of Biophysics. 2010 | Pubmed ID: 20462379 The Life of an MRNA in Space and Time Journal of Cell Science. May, 2010 | Pubmed ID: 20427315 An Unexpected Ending: Noncanonical 3' End Processing Mechanisms RNA (New York, N.Y.). Feb, 2010 | Pubmed ID: 20007330 Long Noncoding RNAs: Functional Surprises from the RNA World Genes & Development. Jul, 2009 | Pubmed ID: 19571179 Nuclear Neighborhoods and Gene Expression Current Opinion in Genetics & Development. Apr, 2009 | Pubmed ID: 19339170 MEN Epsilon/beta Nuclear-retained Non-coding RNAs Are Up-regulated Upon Muscle Differentiation and Are Essential Components of Paraspeckles Genome Research. Mar, 2009 | Pubmed ID: 19106332 3' End Processing of a Long Nuclear-retained Noncoding RNA Yields a TRNA-like Cytoplasmic RNA Cell. Nov, 2008 | Pubmed ID: 19041754 53BP1 Promotes Non-homologous End Joining of Telomeres by Increasing Chromatin Mobility Nature. Nov, 2008 | Pubmed ID: 18931659 Live Cell Dynamics of Promyelocytic Leukemia Nuclear Bodies Upon Entry into and Exit from Mitosis Molecular Biology of the Cell. Jul, 2008 | Pubmed ID: 18480407 Chromatin Dynamics and Gene Positioning Cell. Mar, 2008 | Pubmed ID: 18358806 A Genetic Locus Targeted to the Nuclear Periphery in Living Cells Maintains Its Transcriptional Competence The Journal of Cell Biology. Jan, 2008 | Pubmed ID: 18195101 Identification of Nuclear Dicing Bodies Containing Proteins for MicroRNA Biogenesis in Living Arabidopsis Plants Current Biology : CB. May, 2007 | Pubmed ID: 17442570 Eukaryotic Regulatory RNAs: an Answer to the 'genome Complexity' Conundrum Genes & Development. Jan, 2007 | Pubmed ID: 17210785 SnapShot: Cellular Bodies Cell. Dec, 2006 | Pubmed ID: 17129789 PIAS1 Confers DNA-binding Specificity on the Msx1 Homeoprotein Genes & Development. Apr, 2006 | Pubmed ID: 16600910 Rapid, Diffusional Shuttling of Poly(A) RNA Between Nuclear Speckles and the Nucleoplasm Molecular Biology of the Cell. Mar, 2006 | Pubmed ID: 16371503 Differential Regulation of Strand-specific Transcripts from Arabidopsis Centromeric Satellite Repeats PLoS Genetics. Dec, 2005 | Pubmed ID: 16389298 Regulating Gene Expression Through RNA Nuclear Retention Cell. Oct, 2005 | Pubmed ID: 16239143 Centromere Positioning and Dynamics in Living Arabidopsis Plants Molecular Biology of the Cell. Dec, 2005 | Pubmed ID: 16195344 Hypophosphorylated SR Splicing Factors Transiently Localize Around Active Nucleolar Organizing Regions in Telophase Daughter Nuclei The Journal of Cell Biology. Oct, 2004 | Pubmed ID: 15479736 Stopping for FISH and Chips Along the Chromatin Fiber Superhighway Molecular Cell. Sep, 2004 | Pubmed ID: 15383273 Human Orc2 Localizes to Centrosomes, Centromeres and Heterochromatin During Chromosome Inheritance The EMBO Journal. Jul, 2004 | Pubmed ID: 15215892 Dynamics of Single MRNPs in Nuclei of Living Cells Science (New York, N.Y.). Jun, 2004 | Pubmed ID: 15205532 Proteomic Analysis of Interchromatin Granule Clusters Molecular Biology of the Cell. Aug, 2004 | Pubmed ID: 15169873 Spatial and Temporal Regulation of Condensins I and II in Mitotic Chromosome Assembly in Human Cells Molecular Biology of the Cell. Jul, 2004 | Pubmed ID: 15146063 "On the Move"ments of Nuclear Components in Living Cells Experimental Cell Research. May, 2004 | Pubmed ID: 15120987 Tissue-specific Expression and Dynamic Organization of SR Splicing Factors in Arabidopsis Molecular Biology of the Cell. Jun, 2004 | Pubmed ID: 15034145 From Silencing to Gene Expression: Real-time Analysis in Single Cells Cell. Mar, 2004 | Pubmed ID: 15006351 The Dynamics of Chromosome Organization and Gene Regulation Annual Review of Biochemistry. 2003 | Pubmed ID: 14527325 Rb-mediated Heterochromatin Formation and Silencing of E2F Target Genes During Cellular Senescence Cell. Jun, 2003 | Pubmed ID: 12809602 Nuclear Choreography: Interpretations from Living Cells Current Opinion in Cell Biology. Apr, 2003 | Pubmed ID: 12648670 Sequential Entry of Components of the Gene Expression Machinery into Daughter Nuclei Molecular Biology of the Cell. Mar, 2003 | Pubmed ID: 12631722 A Molecular Dissection of Nuclear Function. Conference on the Dynamic Nucleus: Questions and Implications EMBO Reports. Jan, 2003 | Pubmed ID: 12524514 Disassembly of Interchromatin Granule Clusters Alters the Coordination of Transcription and Pre-mRNA Splicing The Journal of Cell Biology. Feb, 2002 | Pubmed ID: 11827980 Metabolic-energy-dependent Movement of PML Bodies Within the Mammalian Cell Nucleus Nature Cell Biology. Feb, 2002 | Pubmed ID: 11753375 Quantitative Imaging of Pre-mRNA Splicing Factors in Living Cells Molecular Biology of the Cell. Feb, 2000 | Pubmed ID: 10679003 Nucleocytoplasmic Shuttling: a Novel in Vivo Property of Antisense Phosphorothioate Oligodeoxynucleotides Nucleic Acids Research. Jan, 2000 | Pubmed ID: 10606658 Serine Phosphorylation of SR Proteins is Required for Their Recruitment to Sites of Transcription in Vivo The Journal of Cell Biology. Oct, 1998 | Pubmed ID: 9786943 The Perinucleolar Compartment and Transcription The Journal of Cell Biology. Oct, 1998 | Pubmed ID: 9763419 Phosphorothioate Antisense Oligonucleotides Induce the Formation of Nuclear Bodies Molecular Biology of the Cell. May, 1998 | Pubmed ID: 9571236 Role of the Modular Domains of SR Proteins in Subnuclear Localization and Alternative Splicing Specificity The Journal of Cell Biology. Jul, 1997 | Pubmed ID: 9230067 The Dynamic Organization of the Perinucleolar Compartment in the Cell Nucleus The Journal of Cell Biology. Jun, 1997 | Pubmed ID: 9166399 Establishment and Culture of Patient-Derived Breast Organoids Disha Aggarwal1,2, Suzanne Russo1, Payal Naik1, Sonam Bhatia1, David L. Spector1,2 1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University JoVE 64889 Cancer Research
Establishment and Culture of Patient-Derived Breast Organoids Disha Aggarwal1,2, Suzanne Russo1, Payal Naik1, Sonam Bhatia1, David L. Spector1,2 1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University JoVE 64889 Cancer Research