Dieter Egli Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center Columbia University Biography Publications Institution JoVE Articles Dieter Egli has not added a biography. If you are Dieter Egli and would like to personalize this page please email our Author Liaison for assistance. Publications Permanent Neonatal Diabetes-causing Insulin Mutations Have Dominant Negative Effects on Beta Cell Identity Molecular Metabolism. Jan, 2024 | Pubmed ID: 38237895 Incomplete Reprogramming of DNA Replication Timing in Induced Pluripotent Stem Cells Cell Reports. Jan, 2024 | Pubmed ID: 38194345 DNA Replication in Early Mammalian Embryos is Patterned, Predisposing Lamina-associated Regions to Fragility BioRxiv : the Preprint Server for Biology. Dec, 2023 | Pubmed ID: 38234839 Permanent Neonatal Diabetes-causing Insulin Mutations Have Dominant Negative Effects on Beta Cell Identity BioRxiv : the Preprint Server for Biology. Sep, 2023 | Pubmed ID: 37745320 Incomplete Reprogramming of DNA Replication Timing in Induced Pluripotent Stem Cells BioRxiv : the Preprint Server for Biology. Jun, 2023 | Pubmed ID: 37398435 Replication Stress in Mammalian Embryo Development, Differentiation, and Reprogramming Trends in Cell Biology. Oct, 2023 | Pubmed ID: 37202286 ZnT8 Loss of Function Mutation Increases Resistance of Human Embryonic Stem Cell-Derived Beta Cells to Apoptosis in Low Zinc Condition Cells. Mar, 2023 | Pubmed ID: 36980244 Reduced Calcium Levels and Accumulation of Abnormal Insulin Granules in Stem Cell Models of HNF1A Deficiency Communications Biology. Aug, 2022 | Pubmed ID: 35918471 Replication Stress Impairs Chromosome Segregation and Preimplantation Development in Human Embryos Cell. Aug, 2022 | Pubmed ID: 35858625 Comprehensive Analysis of DNA Replication Timing Across 184 Cell Lines Suggests a Role for MCM10 in Replication Timing Regulation Human Molecular Genetics. Aug, 2022 | Pubmed ID: 35394024 Use of Induced Pluripotent Stem Cells to Build Isogenic Systems and Investigate Type 1 Diabetes Frontiers in Endocrinology. 2021 | Pubmed ID: 34858326 Delayed DNA Replication in Haploid Human Embryonic Stem Cells Genome Research. Dec, 2021 | Pubmed ID: 34810218 The Genetic Architecture of DNA Replication Timing in Human Pluripotent Stem Cells Nature Communications. Nov, 2021 | Pubmed ID: 34799581 Heritable Human Genome Editing: Research Progress, Ethical Considerations, and Hurdles to Clinical Practice Cell. Mar, 2021 | Pubmed ID: 33740453 Bardet-Biedl Syndrome Proteins Regulate Intracellular Signaling and Neuronal Function in Patient-specific IPSC-derived Neurons The Journal of Clinical Investigation. Apr, 2021 | Pubmed ID: 33630762 Reduced Replication Fork Speed Promotes Pancreatic Endocrine Differentiation and Controls Graft Size JCI Insight. Mar, 2021 | Pubmed ID: 33529174 Allele-Specific Chromosome Removal After Cas9 Cleavage in Human Embryos Cell. Dec, 2020 | Pubmed ID: 33125898 How Safe Are Universal Pluripotent Stem Cells? Cell Stem Cell. Aug, 2020 | Pubmed ID: 32763183 Detection of Base Analogs Incorporated During DNA Replication by Nanopore Sequencing Nucleic Acids Research. Sep, 2020 | Pubmed ID: 32710620 Pluripotent Stem Cells with Low Differentiation Potential Contain Incompletely Reprogrammed DNA Replication The Journal of Cell Biology. Sep, 2020 | Pubmed ID: 32673399 Lessons Learned from Somatic Cell Nuclear Transfer International Journal of Molecular Sciences. Mar, 2020 | Pubmed ID: 32230814 Efficient SNP Editing in Haploid Human Pluripotent Stem Cells Journal of Assisted Reproduction and Genetics. Apr, 2020 | Pubmed ID: 32162131 How Safe Are Universal Pluripotent Stem Cells? Cell Stem Cell. Mar, 2020 | Pubmed ID: 32142661 GLP-1 Receptor Agonists Synergize with DYRK1A Inhibitors to Potentiate Functional Human β Cell Regeneration Science Translational Medicine. Feb, 2020 | Pubmed ID: 32051230 Physiological Consequences of Transient Hyperleptinemia During Discrete Developmental Periods on Body Weight in Mice Science Translational Medicine. Jan, 2020 | Pubmed ID: 31894105 Murine Perinatal β-Cell Proliferation and the Differentiation of Human Stem Cell-Derived Insulin-Expressing Cells Require NEUROD1 Diabetes. Dec, 2019 | Pubmed ID: 31519700 Distinct Imprinting Signatures and Biased Differentiation of Human Androgenetic and Parthenogenetic Embryonic Stem Cells Cell Stem Cell. Sep, 2019 | Pubmed ID: 31491396 Stimulation of CRISPR-mediated Homology-directed Repair by an Engineered RAD18 Variant Nature Communications. Jul, 2019 | Pubmed ID: 31363085 Induction of α Cell-restricted Gc in Dedifferentiating β Cells Contributes to Stress-induced β-cell Dysfunction JCI Insight. May, 2019 | Pubmed ID: 31120862 Transgenic Substitution with Greater Amberjack Seriola Dumerili Fish Insulin 2 in NOD Mice Reduces Beta Cell Immunogenicity Scientific Reports. Mar, 2019 | Pubmed ID: 30899071 Ciliary Gene RPGRIP1L is Required for Hypothalamic Arcuate Neuron Development JCI Insight. Feb, 2019 | Pubmed ID: 30728336 NanoMod: a Computational Tool to Detect DNA Modifications Using Nanopore Long-read Sequencing Data BMC Genomics. Feb, 2019 | Pubmed ID: 30712508 Combined Inhibition of DYRK1A, SMAD, and Trithorax Pathways Synergizes to Induce Robust Replication in Adult Human Beta Cells Cell Metabolism. Mar, 2019 | Pubmed ID: 30581122 β Cell Replacement After Gene Editing of a Neonatal Diabetes-Causing Mutation at the Insulin Locus Stem Cell Reports. Dec, 2018 | Pubmed ID: 30503261 Pancreatic Beta Cell Differentiation From Human Pluripotent Stem Cells Current Protocols in Human Genetics. Oct, 2018 | Pubmed ID: 30192441 Inter-homologue Repair in Fertilized Human Eggs? Nature. 08, 2018 | Pubmed ID: 30089924 Identification and Analysis of Islet Antigen-Specific CD8 T Cells with T Cell Libraries Journal of Immunology (Baltimore, Md. : 1950). Sep, 2018 | Pubmed ID: 30082321 Compensating Human Subjects Providing Oocytes for Stem Cell Research: 9-year Experience and Outcomes Journal of Assisted Reproduction and Genetics. Jul, 2018 | Pubmed ID: 29872942 ILDR2 Has a Negligible Role in Hepatic Steatosis PloS One. 2018 | Pubmed ID: 29847571 Report of the Key Opinion Leaders Meeting on Stem Cell-derived Beta Cells Transplantation. Aug, 2018 | Pubmed ID: 29781950 β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells Diabetes. Jan, 2018 | Pubmed ID: 28931519 Tying Genetic Stability to Cell Identity Cell Cycle (Georgetown, Tex.). Jun, 2017 | Pubmed ID: 28548589 Genomic Instability During Reprogramming by Nuclear Transfer is DNA Replication Dependent Nature Cell Biology. Apr, 2017 | Pubmed ID: 28263958 Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes Stem Cell Reports. Mar, 2017 | Pubmed ID: 28242217 PC1/3 Deficiency Impacts Pro-opiomelanocortin Processing in Human Embryonic Stem Cell-Derived Hypothalamic Neurons Stem Cell Reports. Feb, 2017 | Pubmed ID: 28132887 Inconsistency in 'three-parent' IVF Study Reproductive Biomedicine Online. Mar, 2017 | Pubmed ID: 28038847 Deficiency in Prohormone Convertase PC1 Impairs Prohormone Processing in Prader-Willi Syndrome The Journal of Clinical Investigation. Jan, 2017 | Pubmed ID: 27941249 Induced Pluripotent Stem Cells (iPSC) Created from Skin Fibroblasts of Patients with Prader-Willi Syndrome (PWS) Retain the Molecular Signature of PWS Stem Cell Research. Nov, 2016 | Pubmed ID: 27789403 Identification and Propagation of Haploid Human Pluripotent Stem Cells Nature Protocols. Nov, 2016 | Pubmed ID: 27763625 Analysis of Chromosomal Aberrations and Recombination by Allelic Bias in RNA-Seq Nature Communications. Jul, 2016 | Pubmed ID: 27385103 Efficient Generation of Hypothalamic Neurons from Human Pluripotent Stem Cells Current Protocols in Human Genetics. Jul, 2016 | Pubmed ID: 27367166 Genetic Drift Can Compromise Mitochondrial Replacement by Nuclear Transfer in Human Oocytes Cell Stem Cell. Jun, 2016 | Pubmed ID: 27212703 Hypomorphism of Fto and Rpgrip1l Causes Obesity in Mice The Journal of Clinical Investigation. May, 2016 | Pubmed ID: 27064284 Derivation and Differentiation of Haploid Human Embryonic Stem Cells Nature. Apr, 2016 | Pubmed ID: 26982723 Attitudes Toward Prevention of MtDNA-related Diseases Through Oocyte Mitochondrial Replacement Therapy Human Reproduction (Oxford, England). May, 2016 | Pubmed ID: 26936885 Human Embryos Commonly Form Abnormal Nuclei During Development: a Mechanism of DNA Damage, Embryonic Aneuploidy, and Developmental Arrest Human Reproduction (Oxford, England). Feb, 2016 | Pubmed ID: 26621855 From Cloned Frogs to Patient Matched Stem Cells: Induced Pluripotency or Somatic Cell Nuclear Transfer? Current Opinion in Genetics & Development. Oct, 2015 | Pubmed ID: 26282611 Toward Beta Cell Replacement for Diabetes The EMBO Journal. Apr, 2015 | Pubmed ID: 25733347 Differentiation of Hypothalamic-like Neurons from Human Pluripotent Stem Cells The Journal of Clinical Investigation. Feb, 2015 | Pubmed ID: 25555215 Stem Cell-Derived Beta Cells for Treatment of Type 1 Diabetes? EBioMedicine. Dec, 2014 | Pubmed ID: 26137512 Comparable Frequencies of Coding Mutations and Loss of Imprinting in Human Pluripotent Cells Derived by Nuclear Transfer and Defined Factors Cell Stem Cell. Nov, 2014 | Pubmed ID: 25517467 FOXO1 Inhibition Yields Functional Insulin-producing Cells in Human Gut Organoid Cultures Nature Communications. 2014 | Pubmed ID: 24979718 Gene Therapy in Patient-specific Stem Cell Lines and a Preclinical Model of Retinitis Pigmentosa with Membrane Frizzled-related Protein Defects Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2014 | Pubmed ID: 24895994 Hypomorphism for RPGRIP1L, a Ciliary Gene Vicinal to the FTO Locus, Causes Increased Adiposity in Mice Cell Metabolism. May, 2014 | Pubmed ID: 24807221 Human Oocytes Reprogram Adult Somatic Nuclei of a Type 1 Diabetic to Diploid Pluripotent Stem Cells Nature. Jun, 2014 | Pubmed ID: 24776804 Hyperphagia: Current Concepts and Future Directions Proceedings of the 2nd International Conference on Hyperphagia Obesity (Silver Spring, Md.). Feb, 2014 | Pubmed ID: 24574081 Validation of Genome-wide Association Study (GWAS)-identified Disease Risk Alleles with Patient-specific Stem Cell Lines Human Molecular Genetics. Jul, 2014 | Pubmed ID: 24497574 β-cell Dysfunction Due to Increased ER Stress in a Stem Cell Model of Wolfram Syndrome Diabetes. Mar, 2014 | Pubmed ID: 24227685 Harnessing the Stem Cell Potential: the Path to Prevent Mitochondrial Disease Nature Medicine. Dec, 2013 | Pubmed ID: 24309656 Mouse BAZ1A (ACF1) is Dispensable for Double-strand Break Repair but is Essential for Averting Improper Gene Expression During Spermatogenesis PLoS Genetics. Nov, 2013 | Pubmed ID: 24244200 Connecting the Cell Cycle with Cellular Identity Cellular Reprogramming. Oct, 2013 | Pubmed ID: 24073943 IPSC-derived β Cells Model Diabetes Due to Glucokinase Deficiency The Journal of Clinical Investigation. Jul, 2013 | Pubmed ID: 23778137 Tying Replication to Cell Identity Nature Reviews. Molecular Cell Biology. Jun, 2013 | Pubmed ID: 23698578 Improved Methods for Reprogramming Human Dermal Fibroblasts Using Fluorescence Activated Cell Sorting PloS One. 2013 | Pubmed ID: 23555815 Nuclear Genome Transfer in Human Oocytes Eliminates Mitochondrial DNA Variants Nature. Jan, 2013 | Pubmed ID: 23254936 Mouse Ooplasm Confers Context-specific Reprogramming Capacity Nature Genetics. Sep, 2012 | Pubmed ID: 22902786 Long-term Safety and Efficacy of Human Induced Pluripotent Stem Cell (iPS) Grafts in a Preclinical Model of Retinitis Pigmentosa Molecular Medicine (Cambridge, Mass.). Aug, 2012 | Pubmed ID: 22895806 BRCA1 Tumor Suppression Depends on BRCT Phosphoprotein Binding, but Not Its E3 Ligase Activity Science (New York, N.Y.). Oct, 2011 | Pubmed ID: 22034435 Impracticality of Egg Donor Recruitment in the Absence of Compensation Cell Stem Cell. Oct, 2011 | Pubmed ID: 21982228 Human Oocytes Reprogram Somatic Cells to a Pluripotent State Nature. Oct, 2011 | Pubmed ID: 21979046 Reprogramming Within Hours Following Nuclear Transfer into Mouse but Not Human Zygotes Nature Communications. 2011 | Pubmed ID: 21971503 The New York Stem Cell Foundation: Fifth Annual Translational Stem Cell Research Conference Annals of the New York Academy of Sciences. May, 2011 | Pubmed ID: 21615750 Recipient Cell Nuclear Factors Are Required for Reprogramming by Nuclear Transfer Development (Cambridge, England). Jun, 2010 | Pubmed ID: 20463036 Breaking Ground on Translational Stem Cell Research Annals of the New York Academy of Sciences. Mar, 2010 | Pubmed ID: 20233361 Overexpression of Metal-responsive Transcription Factor (MTF-1) in Drosophila Melanogaster Ameliorates Life-span Reductions Associated with Oxidative Stress and Metal Toxicity Neurobiology of Aging. Jul, 2010 | Pubmed ID: 18775584 A Small-molecule Inhibitor of Tgf-Beta Signaling Replaces Sox2 in Reprogramming by Inducing Nanog Cell Stem Cell. Nov, 2009 | Pubmed ID: 19818703 Reprogramming After Chromosome Transfer into Mouse Blastomeres Current Biology : CB. Aug, 2009 | Pubmed ID: 19682906 Digital RNA Allelotyping Reveals Tissue-specific and Allele-specific Gene Expression in Human Nature Methods. Aug, 2009 | Pubmed ID: 19620972 Targeted Bisulfite Sequencing Reveals Changes in DNA Methylation Associated with Nuclear Reprogramming Nature Biotechnology. Apr, 2009 | Pubmed ID: 19330000 Optimal Timing of Inner Cell Mass Isolation Increases the Efficiency of Human Embryonic Stem Cell Derivation and Allows Generation of Sibling Cell Lines Cell Stem Cell. Feb, 2009 | Pubmed ID: 19200798 Dumpy-30 Family Members As Determinants of Male Fertility and Interaction Partners of Metal-responsive Transcription Factor 1 (MTF-1) in Drosophila BMC Developmental Biology. 2008 | Pubmed ID: 18588663 Mediators of Reprogramming: Transcription Factors and Transitions Through Mitosis Nature Reviews. Molecular Cell Biology. Jul, 2008 | Pubmed ID: 18568039 CAF-1 is Essential for Drosophila Development and Involved in the Maintenance of Epigenetic Memory Developmental Biology. Nov, 2007 | Pubmed ID: 17916346 Developmental Reprogramming After Chromosome Transfer into Mitotic Mouse Zygotes Nature. Jun, 2007 | Pubmed ID: 17554301 Copper Homeostasis in Drosophila by Complex Interplay of Import, Storage and Behavioral Avoidance The EMBO Journal. Feb, 2007 | Pubmed ID: 17290228 In Vivo Construction of Transgenes in Drosophila Genetics. Apr, 2007 | Pubmed ID: 17179096 Transcriptome Response to Heavy Metal Stress in Drosophila Reveals a New Zinc Transporter That Confers Resistance to Zinc Nucleic Acids Research. 2006 | Pubmed ID: 16973896 The Four Members of the Drosophila Metallothionein Family Exhibit Distinct Yet Overlapping Roles in Heavy Metal Homeostasis and Detoxification Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Jun, 2006 | Pubmed ID: 16716195 A Family Knockout of All Four Drosophila Metallothioneins Reveals a Central Role in Copper Homeostasis and Detoxification Molecular and Cellular Biology. Mar, 2006 | Pubmed ID: 16508004 Metal-responsive Transcription Factor (MTF-1) Handles Both Extremes, Copper Load and Copper Starvation, by Activating Different Genes Genes & Development. Apr, 2005 | Pubmed ID: 15833915 Metal-responsive Transcription Factor (MTF-1) and Heavy Metal Stress Response in Drosophila and Mammalian Cells: a Functional Comparison Biological Chemistry. Jul, 2004 | Pubmed ID: 15318808 An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-strand Breaks in Drosophila Melanogaster Genome Research. Jul, 2004 | Pubmed ID: 15197166 Physical and Functional Interaction Between the Bloom's Syndrome Gene Product and the Largest Subunit of Chromatin Assembly Factor 1 Molecular and Cellular Biology. Jun, 2004 | Pubmed ID: 15143166 Knockout of 'metal-responsive Transcription Factor' MTF-1 in Drosophila by Homologous Recombination Reveals Its Central Role in Heavy Metal Homeostasis The EMBO Journal. Jan, 2003 | Pubmed ID: 12505988 Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells Ines Cherkaoui1, Qian Du2, Dieter Egli2, Shivani Misra1,3, Guy A. Rutter1,4,5 1Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, 2Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, 3Department of Diabetes, Imperial College Healthcare NHS Trust, 4Faculté de Médicine, Université de Montréal, 5Lee Kong Chian Imperial Medical School, Nanyang Technological University JoVE 65530 Biology Nuclear Transfer into Mouse Oocytes JoVE 116 Biology
Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells Ines Cherkaoui1, Qian Du2, Dieter Egli2, Shivani Misra1,3, Guy A. Rutter1,4,5 1Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, 2Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, 3Department of Diabetes, Imperial College Healthcare NHS Trust, 4Faculté de Médicine, Université de Montréal, 5Lee Kong Chian Imperial Medical School, Nanyang Technological University JoVE 65530 Biology