Dirk Grimm Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty University of Heidelberg Biography Publications Institution JoVE Articles Dirk Grimm has not added a biography. If you are Dirk Grimm and would like to personalize this page please email our Author Liaison for assistance. Publications Determination of AAV Properties by Single Amino Acids: Go(o)d is in the Details Molecular Therapy. Methods & Clinical Development. Dec, 2022 | Pubmed ID: 36212908 Semirational Bioengineering of AAV Vectors with Increased Potency and Specificity for Systemic Gene Therapy of Muscle Disorders Science Advances. 09, 2022 | Pubmed ID: 36129972 In Vivo Adenine Base Editing Reverts C282Y and Improves Iron Metabolism in Hemochromatosis Mice Nature Communications. 09, 2022 | Pubmed ID: 36064805 Boosters for Adeno-associated Virus Vector (AAV) (r)evolution Cytotherapy. Aug, 2022 | Pubmed ID: 35999132 Fantastic AAV Gene Therapy Vectors and How to Find Them-Random Diversification, Rational Design and Machine Learning Pathogens (Basel, Switzerland). Jul, 2022 | Pubmed ID: 35890005 Intranasal Application of Adeno-associated Viruses: a Systematic Review Translational Research : the Journal of Laboratory and Clinical Medicine. Oct, 2022 | Pubmed ID: 35597541 Identification of Adeno-associated Virus Variants for Gene Transfer into Human Neural Cell Types by Parallel Capsid Screening Scientific Reports. 05, 2022 | Pubmed ID: 35589936 Right on Target: The Next Class of Efficient, Safe, and Specific RNAi Triggers Molecular Therapy. Nucleic Acids. Jun, 2022 | Pubmed ID: 35505965 Natural Killer Cells Act As an Extrinsic Barrier for in Vivo Reprogramming Development (Cambridge, England). 04, 2022 | Pubmed ID: 35420133 Ex vivo and In vivo Suppression of SARS-CoV-2 with Combinatorial AAV/RNAi Expression Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. 05, 2022 | Pubmed ID: 35038579 An RNA Interference/Adeno-Associated Virus Vector-Based Combinatorial Gene Therapy Approach Against Hepatitis E Virus Hepatology Communications. 04, 2022 | Pubmed ID: 34719133 Breaking the Sound Barrier: Towards Next-generation AAV Vectors for Gene Therapy of Hearing Disorders Hearing Research. 01, 2022 | Pubmed ID: 33268240 Cas9-specific Immune Responses Compromise Local and Systemic AAV CRISPR Therapy in Multiple Dystrophic Canine Models Nature Communications. 11, 2021 | Pubmed ID: 34819506 Adeno-Associated Viruses (AAV) and Host Immunity - A Race Between the Hare and the Hedgehog Frontiers in Immunology. 2021 | Pubmed ID: 34777364 Lentiviral and Adeno-associated Vectors Efficiently Transduce Mouse T Lymphocytes when Targeted to Murine CD8 Molecular Therapy. Methods & Clinical Development. Dec, 2021 | Pubmed ID: 34729380 Neutralizing Antibody Evasion and Transduction with Purified Extracellular Vesicle-Enveloped Adeno-Associated Virus Vectors Human Gene Therapy. 12, 2021 | Pubmed ID: 34445894 The Angiopoietin-Tie2 Pathway Regulates Purkinje Cell Dendritic Morphogenesis in a Cell-autonomous Manner Cell Reports. 08, 2021 | Pubmed ID: 34407407 Membrane-destabilizing Ionizable Phospholipids: Novel Components for Organ-selective MRNA Delivery and CRISPR-Cas Gene Editing Signal Transduction and Targeted Therapy. 05, 2021 | Pubmed ID: 34035211 A Versatile In Vivo System to Study Myc in Cell Reprogramming Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 34019296 Best of Most Possible Worlds: Hybrid Gene Therapy Vectors Based on Parvoviruses and Heterologous Viruses Molecular Therapy : the Journal of the American Society of Gene Therapy. 12, 2021 | Pubmed ID: 33831556 Characterization of the GBoV1 Capsid and Its Antibody Interactions Viruses. 02, 2021 | Pubmed ID: 33672786 A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species Via Fluorescence-activated Cell Sorting Molecular Therapy. Methods & Clinical Development. Mar, 2021 | Pubmed ID: 33665228 'Hit and Run' Therapy Averts Macular Degeneration Nature Biomedical Engineering. 02, 2021 | Pubmed ID: 33580229 Optogenetic Control of Neisseria Meningitidis Cas9 Genome Editing Using an Engineered, Light-switchable Anti-CRISPR Protein Nucleic Acids Research. 03, 2021 | Pubmed ID: 33330940 Production, Processing, and Characterization of Synthetic AAV Gene Therapy Vectors Biotechnology Journal. Jan, 2021 | Pubmed ID: 32975881 Identification of a Myotropic AAV by Massively Parallel in Vivo Evaluation of Barcoded Capsid Variants Nature Communications. 10, 2020 | Pubmed ID: 33116134 Lab-Scale Production of Recombinant Adeno-Associated Viruses (AAV) for Expression of Optogenetic Elements Methods in Molecular Biology (Clifton, N.J.). 2020 | Pubmed ID: 32651911 Computational Design of Anti-CRISPR Proteins with Improved Inhibition Potency Nature Chemical Biology. 07, 2020 | Pubmed ID: 32284602 Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity Journal of Virology. 05, 2020 | Pubmed ID: 32213611 Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening Molecular Therapy : the Journal of the American Society of Gene Therapy. 04, 2020 | Pubmed ID: 32105604 Ex Vivo/In vivo Gene Editing in Hepatocytes Using "All-in-One" CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template IScience. Jan, 2020 | Pubmed ID: 31887661 Distinct Transduction of Muscle Tissue in Mice After Systemic Delivery of AAVpo1 Vectors Gene Therapy. 04, 2020 | Pubmed ID: 31624368 Severe Human Bocavirus 1 Respiratory Tract Infection in an Immunodeficient Child With Fatal Outcome The Pediatric Infectious Disease Journal. 09, 2019 | Pubmed ID: 31033910 Cell-specific CRISPR-Cas9 Activation by MicroRNA-dependent Expression of Anti-CRISPR Proteins Nucleic Acids Research. 07, 2019 | Pubmed ID: 30982889 Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30912043 Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses Molecular Therapy. Methods & Clinical Development. Mar, 2019 | Pubmed ID: 30766894 A Robust and All-Inclusive Pipeline for Shuffling of Adeno-Associated Viruses ACS Synthetic Biology. 01, 2019 | Pubmed ID: 30513195 Impact of the Assembly-Activating Protein on Molecular Evolution of Synthetic Adeno-Associated Virus Capsids Human Gene Therapy. 01, 2019 | Pubmed ID: 29978729 Engineered Anti-CRISPR Proteins for Optogenetic Control of CRISPR-Cas9 Nature Methods. 11, 2018 | Pubmed ID: 30377362 AAVvector-mediated in Vivo Reprogramming into Pluripotency Nature Communications. 07, 2018 | Pubmed ID: 29985406 High-Throughput Dissection of AAV-Host Interactions: The Fast and the Curious Journal of Molecular Biology. 08, 2018 | Pubmed ID: 29782834 Split Cas9, Not Hairs - Advancing the Therapeutic Index of CRISPR Technology Biotechnology Journal. Sep, 2018 | Pubmed ID: 29316283 Regulation of Adult CNS Axonal Regeneration by the Post-transcriptional Regulator Cpeb1 Frontiers in Molecular Neuroscience. 2017 | Pubmed ID: 29379413 Small But Increasingly Mighty: Latest Advances in AAV Vector Research, Design, and Evolution Human Gene Therapy. 11, 2017 | Pubmed ID: 28835125 Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells Journal of Virology. 10, 2017 | Pubmed ID: 28768875 Next-generation AAV Vectors for Clinical Use: an Ever-accelerating Race Virus Genes. Oct, 2017 | Pubmed ID: 28762205 Plasmodium Berghei EXP-1 Interacts with Host Apolipoprotein H During Plasmodium Liver-stage Development Proceedings of the National Academy of Sciences of the United States of America. Feb, 2017 | Pubmed ID: 28137845 TALEN/CRISPR-mediated Engineering of a Promoterless Anti-viral RNAi Hairpin into an Endogenous MiRNA Locus Nucleic Acids Research. 01, 2017 | Pubmed ID: 27614072 Blocking Sense-strand Activity Improves Potency, Safety and Specificity of Anti-hepatitis B Virus Short Hairpin RNA EMBO Molecular Medicine. 09, 2016 | Pubmed ID: 27473329 Death Receptor-based Enrichment of Cas9-expressing Cells BMC Biotechnology. Feb, 2016 | Pubmed ID: 26883910 Zinc Finger Nuclease-based Double-strand Breaks Attenuate Malaria Parasites and Reveal Rare Microhomology-mediated End Joining Genome Biology. Nov, 2015 | Pubmed ID: 26573820 AAV8-mediated in Vivo Overexpression of MiR-155 Enhances the Protective Capacity of Genetically Attenuated Malarial Parasites Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2014 | Pubmed ID: 25189739 Déterminants Expression De Protéines Argonaute Mammifères Dans La Médiation De Gene Silencing Nucleic Acids Research. Dec, 2011 | Pubmed ID: 22210886 Le Destin De Traçage Des Hépatocytes Matures Dans L'homéostasie Du Foie De Souris Et De La Régénération The Journal of Clinical Investigation. Dec, 2011 | Pubmed ID: 22105172 La Dose Peut Faire Le Poison: Leçons Tirées De Défavorable Dans Toxicités Causées Par Surexpression in Vivo ARNi Silence. 2011 | Pubmed ID: 22029761 To Go, Ou Ne Pas Aller, Telle Est La Question - Six Réflexions Personnelles Sur La Mobilité Géographique Comment Peut Affecter Votre Carrière Et De Vie BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Oct, 2011 | Pubmed ID: 21858845 Lorsque Les Réseaux Cellulaires Hors De Contrôle: Dérégulation Mondiale De La Machinerie ARNi En Pathologie Humaine Et De La Thérapie Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846572 Mécanismes Cellulaires Interférence ARN. Préface Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846566 Stabilité Thermodynamique Des ARN En épingle à Cheveux Petites Influence Très Fortement Les Processus De Chargement Des Différents Mammifères Argonautes Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21576459 Protéines Argonaute Sont Des Facteurs Déterminants De L'efficacité Des ARNi, La Toxicité, La Persistance Et à Le Foie Des Souris Adultes The Journal of Clinical Investigation. Sep, 2010 | Pubmed ID: 20697157 Six Virus à ARN Et Quarante Et Un Hôtes: Virales Petits ARN Et De Modulation Des Répertoires Petits ARN Dans Les Systèmes De Vertébrés Et Invertébrés PLoS Pathogens. Feb, 2010 | Pubmed ID: 20169186 Faible Niveau De Cytotoxicité ShRNA Peut Contribuer à La Carcinome Hépatocellulaire MYC-induite Chez La Souris Adulte Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2010 | Pubmed ID: 19844192 ARN Muselage Petit: State-of-the-art Advanced Drug Delivery Reviews. Jul, 2009 | Pubmed ID: 19427885 Expression De ShRNA à Partir D'un Promoteur Tissu-spécifique Pol II Est Un Efficace Et Sûr ARNi Thérapeutiques Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2008 | Pubmed ID: 18665161 Taire De L'acide Hépatique Transporter Protein Gras 5 in Vivo Inverse Induite Par Le Régime Non-stéatose Hépatique Et Améliore L'hyperglycémie The Journal of Biological Chemistry. Aug, 2008 | Pubmed ID: 18524776 In Vitro Et in Vivo Gene Therapy Evolution Vecteur Via Métissage Multispécifique Et Reciblage De Virus Adéno-associés Journal of Virology. Jun, 2008 | Pubmed ID: 18400866 Remplacement Du Parenchyme Hépatique Chez Les Souris Transplantées Par Hépatocytes Allogéniques Est Facilitée Par La Transplantation De Moelle Osseuse Et Médiée Par Les Cellules CD4 Hepatology (Baltimore, Md.). Feb, 2008 | Pubmed ID: 18220289 Application Thérapeutique De L'ARNi: Est L'ARNm De Ciblage Enfin Prêt Pour Prime Time? The Journal of Clinical Investigation. Dec, 2007 | Pubmed ID: 18060021 ARNi Et La Thérapie Génique: Une Attraction Mutuelle Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program. 2007 | Pubmed ID: 18024667 Knockdown Rapide Et Stable D'un Gène Endogène Dans L'épithélium Pigmentaire Rétinien Human Gene Therapy. Oct, 2007 | Pubmed ID: 17892416 Combinatoire ARNi: Une Stratégie Gagnante Pour La Course Contre Des Cibles En évolution? Molecular Therapy : the Journal of the American Society of Gene Therapy. May, 2007 | Pubmed ID: 17311009 Un écran Double-hybride Identifie Cathepsines B Et L Comme Facteurs De Déshabillage Pour Virus Adéno-associé 2 Et 8 Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2007 | Pubmed ID: 17235311 Le Récepteur Laminine 37/67-kilodalton Est Un Récepteur Pour Les Sérotypes De Virus Adéno-associés 8, 2, 3, Et 9 Journal of Virology. Oct, 2006 | Pubmed ID: 16973587 Fatality Chez La Souris En Raison De La Sursaturation Des MicroARN Cellulaires / Court En épingle à Cheveux D'ARN Pathways Nature. May, 2006 | Pubmed ID: 16724069 Amélioration De Transfert De Gène Cardiaque Par Transcriptionnelle Et Transductional Ciblage Des Vecteurs Adéno-associés Virales Cardiovascular Research. Apr, 2006 | Pubmed ID: 16448634 Transduction Du Foie Avec Un Virus Recombinant Adéno-associé Est Principalement Limitée Par Le Sérotype Capside Génotype Vecteur Non Journal of Virology. Jan, 2006 | Pubmed ID: 16352567 Entretien Accrue Et Persistance Des Transgènes Par Excision De Cassettes D'expression De Séquences Plasmidiques Dans Vivo Human Gene Therapy. May, 2005 | Pubmed ID: 15916481 Vecteurs Viraux Adéno-associés Pour En épingle à Cheveux Courte Expression D'ARN Methods in Enzymology. 2005 | Pubmed ID: 15644194 Préclinique in Vivo D'évaluation Des Pseudotypés Vecteurs Viraux Adéno-associés Pour La Thérapie Génique Du Foie Blood. Oct, 2003 | Pubmed ID: 12791653 Helper Virus-free, De La Production Optiquement Contrôlable, Et Deux à Base De Plasmides De Vecteurs Viraux Adéno-associés Des Sérotypes 1 à 6 Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2003 | Pubmed ID: 12788658 Les Méthodes De Production Pour Des Vecteurs De Transfert Génique Basée Sur Sérotypes De Virus Adéno-associés Methods (San Diego, Calif.). Oct, 2002 | Pubmed ID: 12413413 Des Cellules Humaines Primaires Diffèrent Dans Leur Sensibilité Au Transfert De Gènes RAAV-2-médiation Et La Durée Des Expression Du Gène Rapporteur Journal of Virological Methods. Sep, 2002 | Pubmed ID: 12270659 Isolement de vecteurs de thérapie génique de nouvelle génération par l’ingénierie, le codage à barres et le criblage de variantes de capside du virus adéno-associé (AAV) Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengineering Vecteurs d'ingénierie et de l'évolution de la synthèse Adeno-Associated Virus (AAV) thérapie génique par la famille de réarrangement d'ADN Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Immunology and Infection
Isolement de vecteurs de thérapie génique de nouvelle génération par l’ingénierie, le codage à barres et le criblage de variantes de capside du virus adéno-associé (AAV) Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengineering
Vecteurs d'ingénierie et de l'évolution de la synthèse Adeno-Associated Virus (AAV) thérapie génique par la famille de réarrangement d'ADN Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Immunology and Infection