Eric B. Kmiec Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute Christiana Care Health Services Biography Publications Institution JoVE Articles Eric B. Kmiec has not added a biography. If you are Eric B. Kmiec and would like to personalize this page please email our Author Liaison for assistance. Publications CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome Molecular Therapy. Nucleic Acids. Jun, 2017 | Pubmed ID: 28624204 To Cleave or Not to Cleave: Therapeutic Gene Editing with and Without Programmable Nucleases Nature Reviews. Drug Discovery. Apr, 2017 | Pubmed ID: 28303022 Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides PloS One. 2017 | Pubmed ID: 28052104 Analyses of Point Mutation Repair and Allelic Heterogeneity Generated by CRISPR/Cas9 and Single-stranded DNA Oligonucleotides Scientific Reports. Sep, 2016 | Pubmed ID: 27609304 Genetic Spell-checking: Gene Editing Using Single-stranded DNA Oligonucleotides Plant Biotechnology Journal. Feb, 2016 | Pubmed ID: 26402400 Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems PloS One. 2015 | Pubmed ID: 26053390 Is the Age of Genetic Surgery Finally Upon Us? Surgical Oncology. Jun, 2015 | Pubmed ID: 25936245 The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-stranded Oligonucleotides PloS One. 2014 | Pubmed ID: 24788536 Enrichment of Breast Cancer Stem-like Cells by Growth on Electrospun Polycaprolactone-chitosan Nanofiber Scaffolds International Journal of Nanomedicine. 2014 | Pubmed ID: 24570583 Combinatorial Gene Editing in Mammalian Cells Using SsODNs and TALENs Scientific Reports. Jan, 2014 | Pubmed ID: 24445749 Electrospun Fiber Membranes Enable Proliferation of Genetically Modified Cells International Journal of Nanomedicine. 2013 | Pubmed ID: 23467983 DNA Damage Response Pathway and Replication Fork Stress During Oligonucleotide Directed Gene Editing Molecular Therapy. Nucleic Acids. Apr, 2012 | Pubmed ID: 23343929 Proliferation of Genetically Modified Human Cells on Electrospun Nanofiber Scaffolds Molecular Therapy. Nucleic Acids. 2012 | Pubmed ID: 23212298 Oligonucleotide Delivery by Nucleofection Does Not Rescue the Reduced Proliferation Phenotype of Gene-edited Cells Nucleic Acid Therapeutics. Dec, 2012 | Pubmed ID: 23072627 Modifications of P53 and the DNA Damage Response in Cells Expressing Mutant Form of the Protein Huntingtin Journal of Molecular Neuroscience : MN. Oct, 2011 | Pubmed ID: 21465263 Strand Bias Influences the Mechanism of Gene Editing Directed by Single-stranded DNA Oligonucleotides Nucleic Acids Research. Jun, 2011 | Pubmed ID: 21343181 Gene Editing Activity on Extrachromosomal Arrays in C. Elegans Transgenics Gene. Apr, 2011 | Pubmed ID: 21241788 A Stable G-quartet Binds to a Huntingtin Protein Fragment Containing Expanded Polyglutamine Tracks Journal of Neuroscience Research. Feb, 2010 | Pubmed ID: 19705452 DNA Breakage Associated with Targeted Gene Alteration Directed by DNA Oligonucleotides Mutation Research. Oct, 2009 | Pubmed ID: 19463835 Regulation of Targeted Gene Repair by Intrinsic Cellular Processes BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Feb, 2009 | Pubmed ID: 19204988 DNA Breakage and Induction of DNA Damage Response Proteins Precede the Appearance of Visible Mutant Huntingtin Aggregates Journal of Neuroscience Research. Feb, 2009 | Pubmed ID: 18831068 DNA Replication, Cell Cycle Progression and the Targeted Gene Repair Reaction Cell Cycle (Georgetown, Tex.). May, 2008 | Pubmed ID: 18424915 G-rich Oligonucleotides Alter Cell Cycle Progression and Induce Apoptosis Specifically in OE19 Esophageal Tumor Cells Oligonucleotides. 2008 | Pubmed ID: 18321162 Genetic Conversion of an SMN2 Gene to SMN1: a Novel Approach to the Treatment of Spinal Muscular Atrophy Experimental Cell Research. Feb, 2008 | Pubmed ID: 18078930 Recovery of Cell Cycle Delay Following Targeted Gene Repair by Oligonucleotides DNA Repair. Oct, 2007 | Pubmed ID: 17560837 Manipulation of Cell Cycle Progression Can Counteract the Apparent Loss of Correction Frequency Following Oligonucleotide-directed Gene Repair BMC Molecular Biology. Feb, 2007 | Pubmed ID: 17284323 Reduction of Gene Repair by Selenomethionine with the Use of Single-stranded Oligonucleotides BMC Molecular Biology. Jan, 2007 | Pubmed ID: 17257432 Multiple Roles for MSH2 in the Repair of a Deletion Mutation Directed by Modified Single-stranded Oligonucleotides Gene. Jan, 2007 | Pubmed ID: 17113727 Targeted Gene Repair Activates Chk1 and Chk2 and Stalls Replication in Corrected Cells DNA Repair. Apr, 2006 | Pubmed ID: 16414312 A Tolerance of DNA Heterology in the Mammalian Targeted Gene Repair Reaction Oligonucleotides. 2005 | Pubmed ID: 16201904 Reaction Parameters of Targeted Gene Repair in Mammalian Cells Molecular Biotechnology. Mar, 2005 | Pubmed ID: 15767697 Gene Repair in Mammalian Cells is Stimulated by the Elongation of S Phase and Transient Stalling of Replication Forks DNA Repair. Apr, 2005 | Pubmed ID: 15725625 Fluorescent Detection and Isolation of DNA Variants Using Stabilized RecA-coated Oligonucleotides Discovery Medicine. Apr, 2004 | Pubmed ID: 20705000 Double Displacement Loops (double D-loops) Are Templates for Oligonucleotide-directed Mutagenesis and Gene Repair Oligonucleotides. 2004 | Pubmed ID: 15665595 Camptothecin Enhances the Frequency of Oligonucleotide-directed Gene Repair in Mammalian Cells by Inducing DNA Damage and Activating Homologous Recombination Nucleic Acids Research. 2004 | Pubmed ID: 15466591 Modified Single-stranded Oligonucleotides Inhibit Aggregate Formation and Toxicity Induced by Expanded Polyglutamine Journal of Molecular Neuroscience : MN. 2004 | Pubmed ID: 15456939 Enhanced Oligonucleotide-directed Gene Targeting in Mammalian Cells Following Treatment with DNA Damaging Agents Experimental Cell Research. Oct, 2004 | Pubmed ID: 15383324 DNA Replication and Transcription Direct a DNA Strand Bias in the Process of Targeted Gene Repair in Mammalian Cells Journal of Cell Science. Aug, 2004 | Pubmed ID: 15265980 Genetic Re-engineering of Saccharomyces Cerevisiae RAD51 Leads to a Significant Increase in the Frequency of Gene Repair in Vivo Nucleic Acids Research. 2004 | Pubmed ID: 15087488 Enhancement of in Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA Methods in Molecular Biology (Clifton, N.J.). 2004 | Pubmed ID: 14769964 Fluorescent Detection and Isolation of DNA Variants Using Stabilized RecA-coated Oligonucleotides Genome Research. Jan, 2004 | Pubmed ID: 14672976 The Effect of Hydroxyurea and Trichostatin a on Targeted Nucleotide Exchange in Yeast and Mammalian Cells Annals of the New York Academy of Sciences. Dec, 2003 | Pubmed ID: 14751821 Reduction of Htt Inclusion Formation in Strains of Saccharomyces Cerevisiae Deficient in Certain DNA Repair Functions: a Statistical Analysis of Phenotype Experimental Cell Research. Nov, 2003 | Pubmed ID: 14597407 Nuclease Activity of Saccharomyces Cerevisiae Mre11 Functions in Targeted Nucleotide Alteration Applied and Environmental Microbiology. Oct, 2003 | Pubmed ID: 14532083 Targeted Nucleotide Exchange in the CAG Repeat Region of the Human HD Gene Biochemical and Biophysical Research Communications. Oct, 2003 | Pubmed ID: 14521962 Targeted Gene Repair -- in the Arena The Journal of Clinical Investigation. Sep, 2003 | Pubmed ID: 12952907 The Development and Regulation of Gene Repair Nature Reviews. Genetics. Sep, 2003 | Pubmed ID: 12951569 Targeted Nucleotide Repair of Cyc1 Mutations in Saccharomyces Cerevisiae Directed by Modified Single-stranded DNA Oligonucleotides Genetics. Feb, 2003 | Pubmed ID: 12618392 The Synaptic Complex of RecA Protein Participates in Hybridization and Inverse Strand Exchange Reactions Biochemistry. Mar, 2003 | Pubmed ID: 12614159 Efficiency of Chimeraplast Gene Targeting by Direct Nuclear Injection Using a GFP Recovery Assay Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2003 | Pubmed ID: 12597913 DNA Pairing is an Important Step in the Process of Targeted Nucleotide Exchange Nucleic Acids Research. Feb, 2003 | Pubmed ID: 12560486 Nucleotide Replacement at Two Sites Can Be Directed by Modified Single-stranded Oligonucleotides in Vitro and in Vivo Biomolecular Engineering. Jan, 2003 | Pubmed ID: 12485680 Targeted Nucleotide Exchange in Saccharomyces Cerevisiae Directed by Short Oligonucleotides Containing Locked Nucleic Acids Chemistry & Biology. Oct, 2002 | Pubmed ID: 12401492 Rad51p and Rad54p, but Not Rad52p, Elevate Gene Repair in Saccharomyces Cerevisiae Directed by Modified Single-stranded Oligonucleotide Vectors Nucleic Acids Research. Jul, 2002 | Pubmed ID: 12087156 The 'biased' Evolution of Targeted Gene Repair Current Opinion in Molecular Therapeutics. Apr, 2002 | Pubmed ID: 12044039 Strand Bias in Targeted Gene Repair is Influenced by Transcriptional Activity Molecular and Cellular Biology. Jun, 2002 | Pubmed ID: 11997519 Targeted Gene Repair and Its Application to Neurodegenerative Disorders Neuron. Feb, 2002 | Pubmed ID: 11856524 A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells Natalia Rivera-Torres1,2, Eric B. Kmiec1,2 1Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, 2Department of Medical Sciences, University of Delaware JoVE 56195 Genetics
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells Natalia Rivera-Torres1,2, Eric B. Kmiec1,2 1Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, 2Department of Medical Sciences, University of Delaware JoVE 56195 Genetics