Frances H. Arnold Chemistry and Chemical Engineering California Institute of Technology Biography Publications Institution JoVE Articles Frances H. Arnold has not added a biography. If you are Frances H. Arnold and would like to personalize this page please email our Author Liaison for assistance. Publications Comparison of Random Mutagenesis and Semi-rational Designed Libraries for Improved Cytochrome P450 BM3-catalyzed Hydroxylation of Small Alkanes Protein Engineering, Design & Selection : PEDS. Feb, 2012 | Pubmed ID: 22334757 Reversal of NAD(P)H Cofactor Dependence by Protein Engineering Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22144350 A Structural Study of Hypocrea Jecorina Cel5A Protein Science : a Publication of the Protein Society. Nov, 2011 | Pubmed ID: 21898652 Engineered Ketol-acid Reductoisomerase and Alcohol Dehydrogenase Enable Anaerobic 2-methylpropan-1-ol Production at Theoretical Yield in Escherichia Coli Metabolic Engineering. May, 2011 | Pubmed ID: 21515217 Cytochrome P450: Taming a Wild Type Enzyme Current Opinion in Biotechnology. Dec, 2011 | Pubmed ID: 21411308 Self-organization, Layered Structure, and Aggregation Enhance Persistence of a Synthetic Biofilm Consortium PloS One. 2011 | Pubmed ID: 21347422 Improved Product-per-glucose Yields in P450-dependent Propane Biotransformations Using Engineered Escherichia Coli Biotechnology and Bioengineering. Mar, 2011 | Pubmed ID: 21246504 Optimizing Non-natural Protein Function with Directed Evolution Current Opinion in Chemical Biology. Apr, 2011 | Pubmed ID: 21185770 Metal-substituted Protein MRI Contrast Agents Engineered for Enhanced Relaxivity and Ligand Sensitivity Journal of the American Chemical Society. Feb, 2011 | Pubmed ID: 21171606 Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria Applied and Environmental Microbiology. Feb, 2011 | Pubmed ID: 21169454 Enzymatic Functionalization of Carbon-hydrogen Bonds Chemical Society Reviews. Apr, 2011 | Pubmed ID: 21079862 Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates Chembiochem : a European Journal of Chemical Biology. Dec, 2010 | Pubmed ID: 21108271 A General Mechanism for Network-dosage Compensation in Gene Circuits Science (New York, N.Y.). Sep, 2010 | Pubmed ID: 20929850 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [et Al.]. Aug, 2010 | Pubmed ID: 20814931 Consensus Protein Design Without Phylogenetic Bias Journal of Molecular Biology. Jun, 2010 | Pubmed ID: 20433850 Directed Evolution of a Magnetic Resonance Imaging Contrast Agent for Noninvasive Imaging of Dopamine Nature Biotechnology. Mar, 2010 | Pubmed ID: 20190737 Exploring Protein Fitness Landscapes by Directed Evolution Nature Reviews. Molecular Cell Biology. Dec, 2009 | Pubmed ID: 19935669 Chemoenzymatic Elaboration of Monosaccharides Using Engineered Cytochrome P450BM3 Demethylases Proceedings of the National Academy of Sciences of the United States of America. Sep, 2009 | Pubmed ID: 19805336 A Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites and Diversify Lead Compounds Chemistry (Weinheim an Der Bergstrasse, Germany). Nov, 2009 | Pubmed ID: 19774562 Directed Evolution: New Parts and Optimized Function Current Opinion in Biotechnology. Aug, 2009 | Pubmed ID: 19720520 SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability The Journal of Biological Chemistry. Sep, 2009 | Pubmed ID: 19625252 In the Light of Directed Evolution: Pathways of Adaptive Protein Evolution Proceedings of the National Academy of Sciences of the United States of America. Jun, 2009 | Pubmed ID: 19528653 A Family of Thermostable Fungal Cellulases Created by Structure-guided Recombination Proceedings of the National Academy of Sciences of the United States of America. Apr, 2009 | Pubmed ID: 19307582 Directed Enzyme Evolution: Climbing Fitness Peaks One Amino Acid at a Time Current Opinion in Chemical Biology. Feb, 2009 | Pubmed ID: 19249235 Chemo-enzymatic Fluorination of Unactivated Organic Compounds Nature Chemical Biology. Jan, 2009 | Pubmed ID: 19011638 In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6 Applied and Environmental Microbiology. Jan, 2009 | Pubmed ID: 19011057 Engineering Microbial Consortia: a New Frontier in Synthetic Biology Trends in Biotechnology. Sep, 2008 | Pubmed ID: 18675483 Evolutionary History of a Specialized P450 Propane Monooxygenase Journal of Molecular Biology. Nov, 2008 | Pubmed ID: 18619466 A Synthetic Escherichia Coli Predator-prey Ecosystem Molecular Systems Biology. 2008 | Pubmed ID: 18414488 Implications of Rewiring Bacterial Quorum Sensing Applied and Environmental Microbiology. Jan, 2008 | Pubmed ID: 18039819 Engineered Bidirectional Communication Mediates a Consensus in a Microbial Biofilm Consortium Proceedings of the National Academy of Sciences of the United States of America. Oct, 2007 | Pubmed ID: 17959781 Engineered Alkane-hydroxylating Cytochrome P450(BM3) Exhibiting Nativelike Catalytic Properties Angewandte Chemie (International Ed. in English). 2007 | Pubmed ID: 17886313 Engineering by Homologous Recombination: Exploring Sequence and Function Within a Conserved Fold Current Opinion in Structural Biology. Aug, 2007 | Pubmed ID: 17884462 A Diverse Family of Thermostable Cytochrome P450s Created by Recombination of Stabilizing Fragments Nature Biotechnology. Sep, 2007 | Pubmed ID: 17721510 Directed Evolution of Vibrio Fischeri LuxR for Improved Response to Butanoyl-homoserine Lactone Applied and Environmental Microbiology. Sep, 2007 | Pubmed ID: 17675429 Evolution Favors Protein Mutational Robustness in Sufficiently Large Populations BMC Biology. 2007 | Pubmed ID: 17640347 Neutral Genetic Drift Can Alter Promiscuous Protein Functions, Potentially Aiding Functional Evolution Biology Direct. 2007 | Pubmed ID: 17598905 Diversification of Catalytic Function in a Synthetic Family of Chimeric Cytochrome P450s Chemistry & Biology. Mar, 2007 | Pubmed ID: 17379142 Breaking Proteins with Mutations: Threads and Thresholds in Evolution Molecular Systems Biology. 2007 | Pubmed ID: 17262035 Synthetic Gene Circuits: Design with Directed Evolution Annual Review of Biophysics and Biomolecular Structure. 2007 | Pubmed ID: 17243895 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [et Al.]. Jun, 2006 | Pubmed ID: 18429308 Structure-guided SCHEMA Recombination of Distantly Related Beta-lactamases Protein Engineering, Design & Selection : PEDS. Dec, 2006 | Pubmed ID: 17090554 Structural Determinants of the Rate of Protein Evolution in Yeast Molecular Biology and Evolution. Sep, 2006 | Pubmed ID: 16782762 Dual Selection Enhances the Signaling Specificity of a Variant of the Quorum-sensing Transcriptional Activator LuxR Nature Biotechnology. Jun, 2006 | Pubmed ID: 16715074 Enantioselective Alpha-hydroxylation of 2-arylacetic Acid Derivatives and Buspirone Catalyzed by Engineered Cytochrome P450 BM-3 Journal of the American Chemical Society. May, 2006 | Pubmed ID: 16669674 Structure-guided Recombination Creates an Artificial Family of Cytochromes P450 PLoS Biology. May, 2006 | Pubmed ID: 16594730 Protein Stability Promotes Evolvability Proceedings of the National Academy of Sciences of the United States of America. Apr, 2006 | Pubmed ID: 16581913 Fancy Footwork in the Sequence Space Shuffle Nature Biotechnology. Mar, 2006 | Pubmed ID: 16525408 Biosynthesis of Novel Carotenoid Families Based on Unnatural Carbon Backbones: a Model for Diversification of Natural Product Pathways Biochimica Et Biophysica Acta. Feb, 2006 | Pubmed ID: 16478677 Enantioselective Epoxidation of Terminal Alkenes to (R)- and (S)-epoxides by Engineered Cytochromes P450 BM-3 Chemistry (Weinheim an Der Bergstrasse, Germany). Jan, 2006 | Pubmed ID: 16240317 Preparation of Human Metabolites of Propranolol Using Laboratory-evolved Bacterial Cytochromes P450 Biotechnology and Bioengineering. Feb, 2006 | Pubmed ID: 16224788 Why Highly Expressed Proteins Evolve Slowly Proceedings of the National Academy of Sciences of the United States of America. Oct, 2005 | Pubmed ID: 16176987 Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3 Chembiochem : a European Journal of Chemical Biology. Oct, 2005 | Pubmed ID: 16080215 Evolving Strategies for Enzyme Engineering Current Opinion in Structural Biology. Aug, 2005 | Pubmed ID: 16006119 Long-term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat Science (New York, N.Y.). Jul, 2005 | Pubmed ID: 15994559 Why High-error-rate Random Mutagenesis Libraries Are Enriched in Functional and Improved Proteins Journal of Molecular Biology. Jul, 2005 | Pubmed ID: 15939434 Protein-surfactant Film Voltammetry of Wild-type and Mutant Cytochrome P450 BM3 Inorganic Chemistry. Jun, 2005 | Pubmed ID: 15934729 Stereoselective Hydroxylation of an Achiral Cyclopentanecarboxylic Acid Derivative Using Engineered P450s BM-3 Chemical Communications (Cambridge, England). May, 2005 | Pubmed ID: 15900339 A Synthetic Multicellular System for Programmed Pattern Formation Nature. Apr, 2005 | Pubmed ID: 15858574 On the Conservative Nature of Intragenic Recombination Proceedings of the National Academy of Sciences of the United States of America. Apr, 2005 | Pubmed ID: 15809422 Diversifying Carotenoid Biosynthetic Pathways by Directed Evolution Microbiology and Molecular Biology Reviews : MMBR. Mar, 2005 | Pubmed ID: 15755953 Directed Evolution of Vibrio Fischeri LuxR for Increased Sensitivity to a Broad Spectrum of Acyl-homoserine Lactones Molecular Microbiology. Feb, 2005 | Pubmed ID: 15660998 Thermodynamic Prediction of Protein Neutrality Proceedings of the National Academy of Sciences of the United States of America. Jan, 2005 | Pubmed ID: 15644440 Association of Antigens to ISCOMATRIX Adjuvant Using Metal Chelation Leads to Improved CTL Responses Vaccine. Sep, 2004 | Pubmed ID: 15364446 Cobaltocene-mediated Catalytic Monooxygenation Using Holo and Heme Domain Cytochrome P450 BM3 Journal of Inorganic Biochemistry. Sep, 2004 | Pubmed ID: 15337607 Site-directed Protein Recombination As a Shortest-path Problem Protein Engineering, Design & Selection : PEDS. Jul, 2004 | Pubmed ID: 15331774 Reduction of Dioxygen Catalyzed by Pyrene-wired Heme Domain Cytochrome P450 BM3 Electrodes Journal of the American Chemical Society. Aug, 2004 | Pubmed ID: 15315414 SCHEMA-guided Protein Recombination Methods in Enzymology. 2004 | Pubmed ID: 15289059 Functional Evolution and Structural Conservation in Chimeric Cytochromes P450: Calibrating a Structure-guided Approach Chemistry & Biology. Mar, 2004 | Pubmed ID: 15123260 Stability and the Evolvability of Function in a Model Protein Biophysical Journal. May, 2004 | Pubmed ID: 15111394 Programmed Population Control by Cell-cell Communication and Regulated Killing Nature. Apr, 2004 | Pubmed ID: 15064770 Colorimetric High-throughput Assay for Alkene Epoxidation Catalyzed by Cytochrome P450 BM-3 Variant 139-3 Journal of Biomolecular Screening. Mar, 2004 | Pubmed ID: 15006137 Evolution of a Pathway to Novel Long-chain Carotenoids Journal of Bacteriology. Mar, 2004 | Pubmed ID: 14973014 Laboratory Evolution of Cytochrome P450 BM-3 Monooxygenase for Organic Cosolvents Biotechnology and Bioengineering. Feb, 2004 | Pubmed ID: 14748091 Engineering Proteins That Bind, Move, Make and Break DNA Current Opinion in Biotechnology. Dec, 2003 | Pubmed ID: 14662399 Regio- and Enantioselective Alkane Hydroxylation with Engineered Cytochromes P450 BM-3 Journal of the American Chemical Society. Nov, 2003 | Pubmed ID: 14583039 Thermostabilization of a Cytochrome P450 Peroxygenase Chembiochem : a European Journal of Chemical Biology. Sep, 2003 | Pubmed ID: 12964165 Engineering Proteins That Bind, Move, Make and Break DNA Current Opinion in Biotechnology. Aug, 2003 | Pubmed ID: 12943845 Method to Protect a Targeted Amino Acid Residue During Random Mutagenesis Nucleic Acids Research. Aug, 2003 | Pubmed ID: 12907743 Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450 BM-3 Heme Domain Increases Peroxygenase Activity Biotechnology and Bioengineering. Sep, 2003 | Pubmed ID: 12889037 A Self-sufficient Peroxide-driven Hydroxylation Biocatalyst Angewandte Chemie (International Ed. in English). Jul, 2003 | Pubmed ID: 12876749 Library Analysis of SCHEMA-guided Protein Recombination Protein Science : a Publication of the Protein Society. Aug, 2003 | Pubmed ID: 12876318 Staggered Extension Process (StEP) in Vitro Recombination Methods in Molecular Biology (Clifton, N.J.). 2003 | Pubmed ID: 12824608 General Method for Sequence-independent Site-directed Chimeragenesis Journal of Molecular Biology. Jul, 2003 | Pubmed ID: 12823968 A C35 Carotenoid Biosynthetic Pathway Applied and Environmental Microbiology. Jun, 2003 | Pubmed ID: 12788765 Protein Dynamics in a Family of Laboratory Evolved Thermophilic Enzymes Journal of Molecular Biology. Mar, 2003 | Pubmed ID: 12634066 Functional Expression of a Fungal Laccase in Saccharomyces Cerevisiae by Directed Evolution Applied and Environmental Microbiology. Feb, 2003 | Pubmed ID: 12571021 Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases Journal of Biomolecular Screening. Dec, 2002 | Pubmed ID: 14599353 Directed Evolution of a Genetic Circuit Proceedings of the National Academy of Sciences of the United States of America. Dec, 2002 | Pubmed ID: 12451174 Evolution of the C30 Carotenoid Synthase CrtM for Function in a C40 Pathway Journal of Bacteriology. Dec, 2002 | Pubmed ID: 12426357 Laboratory Evolution of a Soluble, Self-sufficient, Highly Active Alkane Hydroxylase Nature Biotechnology. Nov, 2002 | Pubmed ID: 12368811 Modification of Galactose Oxidase to Introduce Glucose 6-oxidase Activity Chembiochem : a European Journal of Chemical Biology. Aug, 2002 | Pubmed ID: 12203977 An Integrated Microfabricated Cell Sorter Analytical Chemistry. Jun, 2002 | Pubmed ID: 12069222 Protein Building Blocks Preserved by Recombination Nature Structural Biology. Jul, 2002 | Pubmed ID: 12042875 Protein Engineering of Oxygenases for Biocatalysis Current Opinion in Chemical Biology. Apr, 2002 | Pubmed ID: 12038995 A Colorimetric Assay to Quantify Dehydrogenase Activity in Crude Cell Lysates Journal of Biomolecular Screening. Apr, 2002 | Pubmed ID: 12006112 Analysis of Shuffled Gene Libraries Journal of Molecular Biology. Feb, 2002 | Pubmed ID: 11866523 High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli Mary F. Farrow1, Frances H. Arnold2 1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology JoVE 2942 Biology
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli Mary F. Farrow1, Frances H. Arnold2 1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology JoVE 2942 Biology