Ina Dobrinski Department of Cpmparative Biology and Experimental Medicine University of Calgary Biography Publications Institution JoVE Articles Ina DobrinskiProfessor Dr. Ina Dobrinski is Professor of Reproductive Biology in the Faculty of Veterinary Medicine and the Cumming School of Medicine at the University of Calgary. She joined the University of Calgary in 2008 after 11 years at the University of Pennsylvania, where she was a Professor of Reproduction, the Director of the Center for Animal Transgenesis and Germ Cell Research, and the Marion Dilley and Robert George Jones Chair in Reproduction.Research in the Dobrinski laboratory is focused on mammalian germline stem cell biology. This lab was the first to establish germ cell transplantation in non-rodent models to transmit a genetic change introduced into germline stem cells to the next generation. The work enables generation of germline gene-edited non-rodent animal models. They also developed xenografting of testis tissue and cells as an accessible in vivo system to study germline stem cells and spermatogenesis. These approaches allow controlled experimentation in donor species such as primates where experiments in whole animals would be logistically and ethically difficult.More recently, her group pioneered testicular organoid technology as a reproducible in vitro model for cell-cell interactions in the mammalian testis. Publications BNC1 is Required for Maintaining Mouse Spermatogenesis Genesis (New York, N.Y. : 2000). Jan, 2012 | Pubmed ID: 22266914 Isolation of Human Male Germ-line Stem Cells Using Enzymatic Digestion and Magnetic-activated Cell Sorting Methods in Molecular Biology (Clifton, N.J.). Month, 2012 | Pubmed ID: 22144235 Characterization of the Porcine Testis-expressed Gene 11 (Tex11) Spermatogenesis. 4, 2011 | Pubmed ID: 22319663 Identification of Spermatogonia by Labeling for UCH-L1 in Whole Mounted Caprine Seminiferous Tubules Molecular Reproduction and Development. Dec, 2011 | Pubmed ID: 22231703 Lymphoid-specific Helicase (HELLS) is Essential for Meiotic Progression in Mouse Spermatocytes Biology of Reproduction. Jun, 2011 | Pubmed ID: 21349825 Development of Bovine Fetal Testis Tissue After Ectopic Xenografting in Mice Journal of Andrology. May-Jun, 2011 | Pubmed ID: 20930193 Xenografting Restores Spermatogenesis to Cryptorchid Testicular Tissue but Does Not Rescue the Phenotype of Idiopathic Testicular Degeneration in the Horse (Equus Caballus) Reproduction, Fertility, and Development. Month, 2010 | Pubmed ID: 20353727 Postnatal Somatic Cell Proliferation and Seminiferous Tubule Maturation in Pigs: a Non-random Event Theriogenology. Jul, 2010 | Pubmed ID: 20189235 Expression Pattern of Acetylated Alpha-tubulin in Porcine Spermatogonia Molecular Reproduction and Development. Apr, 2010 | Pubmed ID: 20043318 Isolation, Characterization, and Culture of Human Spermatogonia Biology of Reproduction. Feb, 2010 | Pubmed ID: 19846602 Asymmetric Distribution of UCH-L1 in Spermatogonia is Associated with Maintenance and Differentiation of Spermatogonial Stem Cells Journal of Cellular Physiology. Aug, 2009 | Pubmed ID: 19388011 Recent Developments in Testis Tissue Xenografting Reproduction (Cambridge, England). Aug, 2009 | Pubmed ID: 19372227 Porcine Embryos Produced After Intracytoplasmic Sperm Injection Using Xenogeneic Pig Sperm from Neonatal Testis Tissue Grafted in Mice Reproduction, Fertility, and Development. Month, 2008 | Pubmed ID: 18842182 Intratubular Transplantation As a Strategy for Establishing Animal Models of Testicular Germ Cell Tumours International Journal of Experimental Pathology. Oct, 2008 | Pubmed ID: 18808526 Production of Donor-derived Sperm After Spermatogonial Stem Cell Transplantation in the Dog Reproduction (Cambridge, England). Dec, 2008 | Pubmed ID: 18768666 Cryopreservation of Boar Semen and Its Future Importance to the Industry Theriogenology. Nov, 2008 | Pubmed ID: 18653225 Maturation of Testicular Tissue from Infant Monkeys After Xenografting into Mice Endocrinology. Oct, 2008 | Pubmed ID: 18566126 Xenografting of Sheep Testis Tissue and Isolated Cells As a Model for Preservation of Genetic Material from Endangered Ungulates Reproduction (Cambridge, England). Jul, 2008 | Pubmed ID: 18390693 Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts Methods in Molecular Biology (Clifton, N.J.). Month, 2008 | Pubmed ID: 18370057 Adeno-associated Virus (AAV)-mediated Transduction of Male Germ Line Stem Cells Results in Transgene Transmission After Germ Cell Transplantation FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Feb, 2008 | Pubmed ID: 17873102 Xenografting of Adult Mammalian Testis Tissue Animal Reproduction Science. Jun, 2008 | Pubmed ID: 17512146 De Novo Morphogenesis of Functional Testis Tissue After Ectopic Transplantation of Isolated Cells Organogenesis. Oct, 2007 | Pubmed ID: 19279705 Transplantation of Germ Cells and Testis Tissue for the Study and Preservation of Fertility Society of Reproduction and Fertility Supplement. Month, 2007 | Pubmed ID: 17644983 Comparison of Global Gene Expression Between Porcine Testis Tissue Xenografts and Porcine Testis in Situ Molecular Reproduction and Development. Jun, 2007 | Pubmed ID: 17133601 Building a Testis: Formation of Functional Testis Tissue After Transplantation of Isolated Porcine (Sus Scrofa) Testis Cells Biology of Reproduction. Jan, 2007 | Pubmed ID: 17005941 Lsh is Required for Meiotic Chromosome Synapsis and Retrotransposon Silencing in Female Germ Cells Nature Cell Biology. Dec, 2006 | Pubmed ID: 17115026 Successful Transplantation of Bovine Testicular Cells to Heterologous Recipients Reproduction (Cambridge, England). Oct, 2006 | Pubmed ID: 17008473 Protein Gene Product 9.5 is a Spermatogonia-specific Marker in the Pig Testis: Application to Enrichment and Culture of Porcine Spermatogonia Molecular Reproduction and Development. Dec, 2006 | Pubmed ID: 16894537 The Length of the Spermatogenic Cycle is Conserved in Porcine and Ovine Testis Xenografts Journal of Andrology. Jul-Aug, 2006 | Pubmed ID: 16598031 Advances and Applications of Germ Cell Transplantation Human Fertility (Cambridge, England). Mar, 2006 | Pubmed ID: 16581717 Effects of 2-hydroxypropyl-beta-cyclodextrin and Cholesterol on Porcine Sperm Viability and Capacitation Status Following Cold Shock or Incubation Molecular Reproduction and Development. May, 2006 | Pubmed ID: 16450405 Recipient Preparation and Mixed Germ Cell Isolation for Spermatogonial Stem Cell Transplantation in Domestic Cats Journal of Andrology. Mar-Apr, 2006 | Pubmed ID: 16304210 Germ Cell Fate and Seminiferous Tubule Development in Bovine Testis Xenografts Reproduction (Cambridge, England). Dec, 2005 | Pubmed ID: 16322552 Depletion of Endogenous Germ Cells in Male Pigs and Goats in Preparation for Germ Cell Transplantation Journal of Andrology. Nov-Dec, 2005 | Pubmed ID: 16291964 Germ Cell Transplantation Seminars in Reproductive Medicine. Aug, 2005 | Pubmed ID: 16059832 Germ Cell Transplantation and Testis Tissue Xenografting in Domestic Animals Animal Reproduction Science. Oct, 2005 | Pubmed ID: 16055282 A Game of Cat and Mouse: Xenografting of Testis Tissue from Domestic Kittens Results in Complete Cat Spermatogenesis in a Mouse Host Journal of Andrology. Nov-Dec, 2004 | Pubmed ID: 15477365 Thyroid Function and Pregnancy Status in Broodmares Journal of the American Veterinary Medical Association. Mar, 2004 | Pubmed ID: 15070060 Bovine Sperm Capacitation: Assessment of Phosphodiesterase Activity and Intracellular Alkalinization on Capacitation-associated Protein Tyrosine Phosphorylation Molecular Reproduction and Development. Apr, 2004 | Pubmed ID: 14991741 Accelerated Maturation of Primate Testis by Xenografting into Mice Biology of Reproduction. May, 2004 | Pubmed ID: 14736818 Fertility and Germline Transmission of Donor Haplotype Following Germ Cell Transplantation in Immunocompetent Goats Biology of Reproduction. Oct, 2003 | Pubmed ID: 12801978 Progeny from Sperm Obtained After Ectopic Grafting of Neonatal Mouse Testes Biology of Reproduction. Jun, 2003 | Pubmed ID: 12606381 Germ Cell Transplantation in Goats Molecular Reproduction and Development. Apr, 2003 | Pubmed ID: 12589654 Sperm from Neonatal Mammalian Testes Grafted in Mice Nature. Aug, 2002 | Pubmed ID: 12181567 Germ Cell Transplantation in Pigs Biology of Reproduction. Jan, 2002 | Pubmed ID: 11751259 Generering av porcin testikulär Organoider med testis specifik arkitektur med hjälp av Microwell kultur Sadman Sakib1, Yang Yu3,4, Anna Voigt2, Mark Ungrin2,3,4, Ina Dobrinski1,2 1Biochemistry and Molecular Biology, University of Calgary, 2Comparative Biology and Experimental Medicine, University of Calgary, 3Biomedical Engineering Graduate Program, University of Calgary, 4Alberta Diabetes Institute, University of Alberta JoVE 60387 Medicine Könscell Transplantation och testiklar Tissue Xenografting hos möss Lin Tang*1, Jose Rafael Rodriguez-Sosa*1, Ina Dobrinski1 1Department of Comparative Biology and Experimental Medicine, University of Calgary JoVE 3545 Biology
Generering av porcin testikulär Organoider med testis specifik arkitektur med hjälp av Microwell kultur Sadman Sakib1, Yang Yu3,4, Anna Voigt2, Mark Ungrin2,3,4, Ina Dobrinski1,2 1Biochemistry and Molecular Biology, University of Calgary, 2Comparative Biology and Experimental Medicine, University of Calgary, 3Biomedical Engineering Graduate Program, University of Calgary, 4Alberta Diabetes Institute, University of Alberta JoVE 60387 Medicine
Könscell Transplantation och testiklar Tissue Xenografting hos möss Lin Tang*1, Jose Rafael Rodriguez-Sosa*1, Ina Dobrinski1 1Department of Comparative Biology and Experimental Medicine, University of Calgary JoVE 3545 Biology