Inhwan Hwang Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology Biography Publications Institution JoVE Articles Inhwan Hwang has not added a biography. If you are Inhwan Hwang and would like to personalize this page please email our Author Liaison for assistance. Publications Cost-effective Production of Tag-less Recombinant Protein in Nicotiana Benthamiana Plant Biotechnology Journal. Nov, 2018 | Pubmed ID: 30468023 Expression of Seven Carbonic Anhydrases in Red Alga Gracilariopsis Chorda and Their Subcellular Localization in a Heterologous System, Arabidopsis Thaliana Plant Cell Reports. Nov, 2018 | Pubmed ID: 30446790 Jasmonic Acid-inducible TSA1 Facilitates ER Body Formation The Plant Journal : for Cell and Molecular Biology. Sep, 2018 | Pubmed ID: 30267434 Fusion of a Highly N-glycosylated Polypeptide Increases the Expression of ER-localized Proteins in Plants Scientific Reports. Mar, 2018 | Pubmed ID: 29545574 Physiological and Molecular Processes Associated with Long Duration of ABA Treatment Frontiers in Plant Science. 2018 | Pubmed ID: 29515601 Evolution and Design Principles of the Diverse Chloroplast Transit Peptides Molecules and Cells. Mar, 2018 | Pubmed ID: 29487274 AtCAP2 is Crucial for Lytic Vacuole Biogenesis During Germination by Positively Regulating Vacuolar Protein Trafficking Proceedings of the National Academy of Sciences of the United States of America. 02, 2018 | Pubmed ID: 29378957 The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the Trans-Golgi Network/Early Endosome to the Plasma Membrane Molecular Plant. 04, 2018 | Pubmed ID: 29317286 Prolines in Transit Peptides Are Crucial for Efficient Preprotein Translocation into Chloroplasts Plant Physiology. 01, 2018 | Pubmed ID: 29158328 Evolution of Rubisco Complex Small Subunit Transit Peptides from Algae to Plants Scientific Reports. Aug, 2017 | Pubmed ID: 28839179 SCYL2 Genes Are Involved in Clathrin-Mediated Vesicle Trafficking and Essential for Plant Growth Plant Physiology. Sep, 2017 | Pubmed ID: 28751315 Sorting of Nuclear-encoded Chloroplast Membrane Proteins Current Opinion in Plant Biology. 12, 2017 | Pubmed ID: 28668581 SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation During Cell Plate Formation The Plant Cell. Jun, 2017 | Pubmed ID: 28584166 The Prenylated Rab GTPase Receptor PRA1.F4 Contributes to Protein Exit from the Golgi Apparatus Plant Physiology. Jul, 2017 | Pubmed ID: 28487479 Transit Peptide Elements Mediate Selective Protein Targeting to Two Different Types of Chloroplasts in the Single-cell C4 Species Bienertia Sinuspersici Scientific Reports. 01, 2017 | Pubmed ID: 28112241 Spatial Regulation of ABCG25, an ABA Exporter, Is an Important Component of the Mechanism Controlling Cellular ABA Levels The Plant Cell. 10, 2016 | Pubmed ID: 27697789 Arabidopsis BAG1 Functions As a Cofactor in Hsc70-Mediated Proteasomal Degradation of Unimported Plastid Proteins Molecular Plant. 10, 2016 | Pubmed ID: 27343446 Interactions Between Transmembrane Helices Within Monomers of the Aquaporin AtPIP2;1 Play a Crucial Role in Tetramer Formation Molecular Plant. Jul, 2016 | Pubmed ID: 27142778 Cytochrome B5 Reductase 1 Triggers Serial Reactions That Lead to Iron Uptake in Plants Molecular Plant. Apr, 2016 | Pubmed ID: 26712506 Physiological Functions of the COPI Complex in Higher Plants Molecules and Cells. Oct, 2015 | Pubmed ID: 26434491 Abscisic Acid: Biosynthesis, Inactivation, Homoeostasis and Signalling Essays in Biochemistry. 2015 | Pubmed ID: 26374885 Sequence Motifs in Transit Peptides Act As Independent Functional Units and Can Be Transferred to New Sequence Contexts Plant Physiology. Sep, 2015 | Pubmed ID: 26149569 Cytosolic Targeting Factor AKR2A Captures Chloroplast Outer Membrane-localized Client Proteins at the Ribosome During Translation Nature Communications. Apr, 2015 | Pubmed ID: 25880450 Iron Nanoparticle-induced Activation of Plasma Membrane H(+)-ATPase Promotes Stomatal Opening in Arabidopsis Thaliana Environmental Science & Technology. Jan, 2015 | Pubmed ID: 25496563 Targeting and Biogenesis of Transporters and Channels in Chloroplast Envelope Membranes: Unsolved Questions Cell Calcium. Jul, 2015 | Pubmed ID: 25465895 Oral Immunization of Haemaggulutinin H5 Expressed in Plant Endoplasmic Reticulum with Adjuvant Saponin Protects Mice Against Highly Pathogenic Avian Influenza A Virus Infection Plant Biotechnology Journal. Jan, 2015 | Pubmed ID: 25065685 Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar Proteins in Plant Cells Plants (Basel, Switzerland). Aug, 2014 | Pubmed ID: 27135510 An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting Through Coincident Binding of Two Chloroplast Lipids Developmental Cell. Sep, 2014 | Pubmed ID: 25203210 Specific Targeting of Proteins to Outer Envelope Membranes of Endosymbiotic Organelles, Chloroplasts, and Mitochondria Frontiers in Plant Science. 2014 | Pubmed ID: 24808904 Abscisic Acid Uridine Diphosphate Glucosyltransferases Play a Crucial Role in Abscisic Acid Homeostasis in Arabidopsis Plant Physiology. May, 2014 | Pubmed ID: 24676855 The Immediate Upstream Region of the 5'-UTR from the AUG Start Codon Has a Pronounced Effect on the Translational Efficiency in Arabidopsis Thaliana Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24084084 The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with BZIP-type Transcription Factors in Dehydration and Osmotic Stress Responses The Plant Cell. Nov, 2013 | Pubmed ID: 24285786 AtHSP17.8 Overexpression in Transgenic Lettuce Gives Rise to Dehydration and Salt Stress Resistance Phenotypes Through Modulation of ABA-mediated Signaling Plant Cell Reports. Dec, 2013 | Pubmed ID: 24081610 The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of Brassinosteroid Insensitive1 in Arabidopsis The Plant Cell. Aug, 2013 | Pubmed ID: 23975899 Adaptor Protein Complex 2-mediated Endocytosis is Crucial for Male Reproductive Organ Development in Arabidopsis The Plant Cell. Aug, 2013 | Pubmed ID: 23975898 In Vivo Localization in Arabidopsis Protoplasts and Root Tissue Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23913041 Arabidopsis μ-adaptin Subunit AP1M of Adaptor Protein Complex 1 Mediates Late Secretory and Vacuolar Traffic and is Required for Growth Proceedings of the National Academy of Sciences of the United States of America. Jun, 2013 | Pubmed ID: 23733933 Silencing of Nicotiana Benthamiana Neuroblastoma-Amplified Gene Causes ER Stress and Cell Death BMC Plant Biology. Apr, 2013 | Pubmed ID: 23621803 ABA Homeostasis and Signaling Involving Multiple Subcellular Compartments and Multiple Receptors Plant Cell Reports. Jun, 2013 | Pubmed ID: 23430173 Direct Targeting of Proteins from the Cytosol to Organelles: the ER Versus Endosymbiotic Organelles Traffic (Copenhagen, Denmark). Jun, 2013 | Pubmed ID: 23331847 Recruitment of Arf1-GDP to Golgi by Glo3p-type ArfGAPs is Crucial for Golgi Maintenance and Plant Growth Plant Physiology. Feb, 2013 | Pubmed ID: 23266962 Cytosolic Events Involved in Chloroplast Protein Targeting Biochimica Et Biophysica Acta. Feb, 2013 | Pubmed ID: 22450030 Trafficking of Vacuolar Proteins: the Crucial Role of Arabidopsis Vacuolar Protein Sorting 29 in Recycling Vacuolar Sorting Receptor The Plant Cell. Dec, 2012 | Pubmed ID: 23263768 Mitochondrial Targeting of the Arabidopsis F1-ATPase γ-subunit Via Multiple Compensatory and Synergistic Presequence Motifs The Plant Cell. Dec, 2012 | Pubmed ID: 23250447 Functional Identification of Sorting Receptors Involved in Trafficking of Soluble Lytic Vacuolar Proteins in Vegetative Cells of Arabidopsis Plant Physiology. Nov, 2012 | Pubmed ID: 23175753 An A/ENTH Domain-containing Protein Functions As an Adaptor for Clathrin-coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells Plant Physiology. Jul, 2012 | Pubmed ID: 22635117 A Vacuolar β-glucosidase Homolog That Possesses Glucose-conjugated Abscisic Acid Hydrolyzing Activity Plays an Important Role in Osmotic Stress Responses in Arabidopsis The Plant Cell. May, 2012 | Pubmed ID: 22582100 Secretory Pathway Research: the More Experimental Systems the Better The Plant Cell. Apr, 2012 | Pubmed ID: 22523202 Identification of Sorting Motifs of AtβFruct4 for Trafficking from the ER to the Vacuole Through the Golgi and PVC Traffic (Copenhagen, Denmark). Dec, 2011 | Pubmed ID: 21899678 An Arabidopsis Prenylated Rab Acceptor 1 Isoform, AtPRA1.B6, Displays Differential Inhibitory Effects on Anterograde Trafficking of Proteins at the Endoplasmic Reticulum Plant Physiology. Oct, 2011 | Pubmed ID: 21828250 Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21822832 Small Heat Shock Protein Hsp17.8 Functions As an AKR2A Cofactor in the Targeting of Chloroplast Outer Membrane Proteins in Arabidopsis Plant Physiology. Sep, 2011 | Pubmed ID: 21730198 The AP-3 Adaptor Complex is Required for Vacuolar Function in Arabidopsis Cell Research. Dec, 2011 | Pubmed ID: 21670741 Both the Hydrophobicity and a Positively Charged Region Flanking the C-terminal Region of the Transmembrane Domain of Signal-anchored Proteins Play Critical Roles in Determining Their Targeting Specificity to the Endoplasmic Reticulum or Endosymbiotic Organelles in Arabidopsis Cells The Plant Cell. Apr, 2011 | Pubmed ID: 21515817 Localization and Trafficking of an Isoform of the AtPRA1 Family to the Golgi Apparatus Depend on Both N- and C-terminal Sequence Motifs Traffic (Copenhagen, Denmark). Feb, 2011 | Pubmed ID: 21059161 Phosphoinositides Regulate Clathrin-dependent Endocytosis at the Tip of Pollen Tubes in Arabidopsis and Tobacco The Plant Cell. Dec, 2010 | Pubmed ID: 21189293 Molecular Association of the Arabidopsis ETR1 Ethylene Receptor and a Regulator of Ethylene Signaling, RTE1 The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20952388 Homomeric Interaction of AtVSR1 is Essential for Its Function As a Vacuolar Sorting Receptor Plant Physiology. Sep, 2010 | Pubmed ID: 20625000 Heat Shock Protein Cognate 70-4 and an E3 Ubiquitin Ligase, CHIP, Mediate Plastid-destined Precursor Degradation Through the Ubiquitin-26S Proteasome System in Arabidopsis The Plant Cell. Dec, 2009 | Pubmed ID: 20028838 Transport Vesicle Formation in Plant Cells Current Opinion in Plant Biology. Dec, 2009 | Pubmed ID: 19854098 Multiple Sequence Motifs in the Rubisco Small Subunit Transit Peptide Independently Contribute to Toc159-dependent Import of Proteins into Chloroplasts Plant Physiology. Sep, 2009 | Pubmed ID: 19571307 Disruption of Glycosylphosphatidylinositol-anchored Lipid Transfer Protein Gene Altered Cuticular Lipid Composition, Increased Plastoglobules, and Enhanced Susceptibility to Infection by the Fungal Pathogen Alternaria Brassicicola Plant Physiology. May, 2009 | Pubmed ID: 19321705 Drought Stress-induced Rma1H1, a RING Membrane-anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels Via Ubiquitination in Transgenic Arabidopsis Plants The Plant Cell. Feb, 2009 | Pubmed ID: 19234086 A Toc159 Import Receptor Mutant, Defective in Hydrolysis of GTP, Supports Preprotein Import into Chloroplasts The Journal of Biological Chemistry. Mar, 2009 | Pubmed ID: 19188370 Sorting and Anterograde Trafficking at the Golgi Apparatus Plant Physiology. Oct, 2008 | Pubmed ID: 18838501 AKR2A-mediated Import of Chloroplast Outer Membrane Proteins is Essential for Chloroplast Biogenesis Nature Cell Biology. Feb, 2008 | Pubmed ID: 18193034 Overexpression of Arabidopsis AGD7 Causes Relocation of Golgi-localized Proteins to the Endoplasmic Reticulum and Inhibits Protein Trafficking in Plant Cells Plant Physiology. Apr, 2007 | Pubmed ID: 17307897 EpsinR2 Interacts with Clathrin, Adaptor Protein-3, AtVTI12, and Phosphatidylinositol-3-phosphate. Implications for EpsinR2 Function in Protein Trafficking in Plant Cells Plant Physiology. Apr, 2007 | Pubmed ID: 17277094 Wax-deficient Anther1 is Involved in Cuticle and Wax Production in Rice Anther Walls and is Required for Pollen Development The Plant Cell. Nov, 2006 | Pubmed ID: 17138699 A WD40 Repeat Protein, Arabidopsis Sec13 Homolog 1, May Play a Role in Vacuolar Trafficking by Controlling the Membrane Association of AtDRP2A Molecules and Cells. Oct, 2006 | Pubmed ID: 17085974 Activation of Glucosidase Via Stress-induced Polymerization Rapidly Increases Active Pools of Abscisic Acid Cell. Sep, 2006 | Pubmed ID: 16990135 Localization of Green Fluorescent Protein Fusions with the Seven Arabidopsis Vacuolar Sorting Receptors to Prevacuolar Compartments in Tobacco BY-2 Cells Plant Physiology. Nov, 2006 | Pubmed ID: 16980567 Arabidopsis EPSIN1 Plays an Important Role in Vacuolar Trafficking of Soluble Cargo Proteins in Plant Cells Via Interactions with Clathrin, AP-1, VTI11, and VSR1 The Plant Cell. Sep, 2006 | Pubmed ID: 16905657 Tic21 is an Essential Translocon Component for Protein Translocation Across the Chloroplast Inner Envelope Membrane The Plant Cell. Sep, 2006 | Pubmed ID: 16891400 Functional Characterization of Sequence Motifs in the Transit Peptide of Arabidopsis Small Subunit of Rubisco Plant Physiology. Feb, 2006 | Pubmed ID: 16384899 Dual Targeting of Xylanase to Chloroplasts and Peroxisomes As a Means to Increase Protein Accumulation in Plant Cells Journal of Experimental Botany. 2006 | Pubmed ID: 16317036 Arabidopsis Calcium-dependent Protein Kinase AtCPK32 Interacts with ABF4, a Transcriptional Regulator of Abscisic Acid-responsive Gene Expression, and Modulates Its Activity Plant Physiology. Dec, 2005 | Pubmed ID: 16299177 Rice Undeveloped Tapetum1 is a Major Regulator of Early Tapetum Development The Plant Cell. Oct, 2005 | Pubmed ID: 16141453 AtRMR1 Functions As a Cargo Receptor for Protein Trafficking to the Protein Storage Vacuole The Journal of Cell Biology. Aug, 2005 | Pubmed ID: 16115960 Multiple Forms of Secretory Phospholipase A2 in Plants Progress in Lipid Research. Jan, 2005 | Pubmed ID: 15748654 Actin Filaments Play a Critical Role in Vacuolar Trafficking at the Golgi Complex in Plant Cells The Plant Cell. Mar, 2005 | Pubmed ID: 15722471 Crystal Structure of Bet3 Reveals a Novel Mechanism for Golgi Localization of Tethering Factor TRAPP Nature Structural & Molecular Biology. Jan, 2005 | Pubmed ID: 15608655 Arabidopsis Metallothioneins 2a and 3 Enhance Resistance to Cadmium when Expressed in Vicia Faba Guard Cells Plant Molecular Biology. Apr, 2004 | Pubmed ID: 15604653 The Arabidopsis Rab5 Homologs Rha1 and Ara7 Localize to the Prevacuolar Compartment Plant & Cell Physiology. Sep, 2004 | Pubmed ID: 15509844 DNA Gyrase is Involved in Chloroplast Nucleoid Partitioning The Plant Cell. Oct, 2004 | Pubmed ID: 15367714 The Transmembrane Domain of AtToc64 and Its C-terminal Lysine-rich Flanking Region Are Targeting Signals to the Chloroplast Outer Envelope Membrane [correction] Molecules and Cells. Apr, 2004 | Pubmed ID: 15179043 Identification of the Protein Storage Vacuole and Protein Targeting to the Vacuole in Leaf Cells of Three Plant Species Plant Physiology. Feb, 2004 | Pubmed ID: 14730078 Localization of VEGFR-2 and PLD2 in Endothelial Caveolae is Involved in VEGF-induced Phosphorylation of MEK and ERK American Journal of Physiology. Heart and Circulatory Physiology. May, 2004 | Pubmed ID: 14704231 The Arabidopsis Dynamin-like Proteins ADL1C and ADL1E Play a Critical Role in Mitochondrial Morphogenesis The Plant Cell. Oct, 2003 | Pubmed ID: 14523248 Functional Expression of a Bacterial Heavy Metal Transporter in Arabidopsis Enhances Resistance to and Decreases Uptake of Heavy Metals Plant Physiology. Oct, 2003 | Pubmed ID: 14512517 The M Domain of AtToc159 Plays an Essential Role in the Import of Proteins into Chloroplasts and Chloroplast Biogenesis The Journal of Biological Chemistry. Sep, 2003 | Pubmed ID: 12853455 Rha1, an Arabidopsis Rab5 Homolog, Plays a Critical Role in the Vacuolar Trafficking of Soluble Cargo Proteins The Plant Cell. May, 2003 | Pubmed ID: 12724533 In Vivo Import Experiments in Protoplasts Reveal the Importance of the Overall Context but Not Specific Amino Acid Residues of the Transit Peptide During Import into Chloroplasts Molecules and Cells. Dec, 2002 | Pubmed ID: 12521302 Phosphatidylinositol 3- and 4-phosphate Are Required for Normal Stomatal Movements The Plant Cell. Oct, 2002 | Pubmed ID: 12368494 ADP-ribosylation Factor 1 of Arabidopsis Plays a Critical Role in Intracellular Trafficking and Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis Plant Physiology. Aug, 2002 | Pubmed ID: 12177464 The Intermolecular Interaction Between the PH Domain and the C-terminal Domain of Arabidopsis Dynamin-like 6 Determines Lipid Binding Specificity The Journal of Biological Chemistry. Aug, 2002 | Pubmed ID: 12105222 Arabidopsis Dynamin-like 2 That Binds Specifically to Phosphatidylinositol 4-phosphate Assembles into a High-molecular Weight Complex in Vivo and in Vitro Plant Physiology. Nov, 2001 | Pubmed ID: 11706203 Identification of a Signal That Distinguishes Between the Chloroplast Outer Envelope Membrane and the Endomembrane System in Vivo The Plant Cell. Oct, 2001 | Pubmed ID: 11595795 A New Dynamin-like Protein, ADL6, is Involved in Trafficking from the Trans-Golgi Network to the Central Vacuole in Arabidopsis The Plant Cell. Jul, 2001 | Pubmed ID: 11449048 Trafficking of Phosphatidylinositol 3-phosphate from the Trans-Golgi Network to the Lumen of the Central Vacuole in Plant Cells The Plant Cell. Feb, 2001 | Pubmed ID: 11226186 Studying Protein Import into Chloroplasts Using Protoplasts Junho Lee1, Hyangju Kang1, Inhwan Hwang1,2 1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology JoVE 58441 Biochemistry
Studying Protein Import into Chloroplasts Using Protoplasts Junho Lee1, Hyangju Kang1, Inhwan Hwang1,2 1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology JoVE 58441 Biochemistry