Jason Moffat Donnelly Centre University of Toronto Biography Publications Institution JoVE Articles Jason Moffat has not added a biography. If you are Jason Moffat and would like to personalize this page please email our Author Liaison for assistance. Publications CRISPR Screens Are Feasible in TP53 Wild-type Cells Molecular Systems Biology. Aug, 2019 | Pubmed ID: 31464370 Tailored Tetravalent Antibodies Potently and Specifically Activate Wnt/Frizzled Pathways in Cells, Organoids and Mice ELife. Aug, 2019 | Pubmed ID: 31452509 Identifying Chemogenetic Interactions from CRISPR Screens with DrugZ Genome Medicine. Aug, 2019 | Pubmed ID: 31439014 Bmi1 Regulates Human Glioblastoma Stem Cells Through Activation of Differential Gene Networks in CD133+ Brain Tumor Initiating Cells Journal of Neuro-oncology. Jul, 2019 | Pubmed ID: 31115870 Genetic Interaction Networks in Cancer Cells Current Opinion in Genetics & Development. Apr, 2019 | Pubmed ID: 30974317 Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Reports. Apr, 2019 | Pubmed ID: 30970261 Global Genetic Networks and the Genotype-to-Phenotype Relationship Cell. Mar, 2019 | Pubmed ID: 30901552 Structure-guided Design Fine-tunes Pharmacokinetics, Tolerability, and Antitumor Profile of Multispecific Frizzled Antibodies Proceedings of the National Academy of Sciences of the United States of America. 04, 2019 | Pubmed ID: 30894493 Yeast Two-Hybrid Analysis for Ubiquitin Variant Inhibitors of Human Deubiquitinases Journal of Molecular Biology. Mar, 2019 | Pubmed ID: 30763569 Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15 Structure (London, England : 1993). Apr, 2019 | Pubmed ID: 30713027 A Rapid in Vitro Methodology for Simultaneous Target Discovery and Antibody Generation Against Functional Cell Subpopulations Scientific Reports. Jan, 2019 | Pubmed ID: 30696911 Functional Genomic Characterization of a Synthetic Anti-HER3 Antibody Reveals a Role for Ubiquitination by RNF41 in the Anti-proliferative Response The Journal of Biological Chemistry. 01, 2019 | Pubmed ID: 30523157 Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30324523 EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30324522 Protocadherin-1 is Essential for Cell Entry by New World Hantaviruses Nature. 11, 2018 | Pubmed ID: 30464266 Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons Molecular Cell. 11, 2018 | Pubmed ID: 30388412 A Synthetic Anti-Frizzled Antibody Engineered for Broadened Specificity Exhibits Enhanced Anti-tumor Properties MAbs. Nov-Dec, 2018 | Pubmed ID: 30183492 The Shieldin Complex Mediates 53BP1-dependent DNA Repair Nature. 08, 2018 | Pubmed ID: 30022168 CRISPR Screens Identify Genomic Ribonucleotides As a Source of PARP-trapping Lesions Nature. 07, 2018 | Pubmed ID: 29973717 Cotargeting Ephrin Receptor Tyrosine Kinases A2 and A3 in Cancer Stem Cells Reduces Growth of Recurrent Glioblastoma Cancer Research. Sep, 2018 | Pubmed ID: 29945963 Map of Synthetic Rescue Interactions for the Fanconi Anemia DNA Repair Pathway Identifies USP48 Nature Communications. 06, 2018 | Pubmed ID: 29891926 The Inhibitory NKR-P1B:Clr-b Recognition Axis Facilitates Detection of Oncogenic Transformation and Cancer Immunosurveillance Cancer Research. 07, 2018 | Pubmed ID: 29691253 Highly Multiplexed and Quantitative Cell-surface Protein Profiling Using Genetically Barcoded Antibodies Proceedings of the National Academy of Sciences of the United States of America. 03, 2018 | Pubmed ID: 29476010 Emerging and Evolving Concepts in Gene Essentiality Nature Reviews. Genetics. Jan, 2018 | Pubmed ID: 29033457 RNAi Screen Identifies Essential Regulators of Human Brain Metastasis-initiating Cells Acta Neuropathologica. 12, 2017 | Pubmed ID: 28766011 Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens G3 (Bethesda, Md.). 08, 2017 | Pubmed ID: 28655737 Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10 Journal of Molecular Biology. Nov, 2017 | Pubmed ID: 28587923 SMYD2 Lysine Methyltransferase Regulates Leukemia Cell Growth and Regeneration After Genotoxic Stress Oncotarget. Mar, 2017 | Pubmed ID: 28187429 Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors Molecular Cell. Feb, 2017 | Pubmed ID: 28157508 Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells ACS Synthetic Biology. 04, 2017 | Pubmed ID: 28033709 Genome-wide CRISPR Screens Reveal a Wnt-FZD5 Signaling Circuit As a Druggable Vulnerability of RNF43-mutant Pancreatic Tumors Nature Medicine. Jan, 2017 | Pubmed ID: 27869803 Kin17 Facilitates Multiple Double-strand Break Repair Pathways That Govern B Cell Class Switching Scientific Reports. 11, 2016 | Pubmed ID: 27853268 Efficient Generation of Patient-matched Malignant and Normal Primary Cell Cultures from Clear Cell Renal Cell Carcinoma Patients: Clinically Relevant Models for Research and Personalized Medicine BMC Cancer. 07, 2016 | Pubmed ID: 27422173 The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination Through ATM and DNAPK-Mediated γH2AX Formation Cell Reports. 05, 2016 | Pubmed ID: 27160905 BAGEL: a Computational Framework for Identifying Essential Genes from Pooled Library Screens BMC Bioinformatics. Apr, 2016 | Pubmed ID: 27083490 Inhibition of SCF Ubiquitin Ligases by Engineered Ubiquitin Variants That Target the Cul1 Binding Site on the Skp1-F-box Interface Proceedings of the National Academy of Sciences of the United States of America. Mar, 2016 | Pubmed ID: 26976582 System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes Molecular Cell. Apr, 2016 | Pubmed ID: 26949039 Pooled Screening for Antiproliferative Inhibitors of Protein-protein Interactions Nature Chemical Biology. Apr, 2016 | Pubmed ID: 26900867 Cytokinetic Effects of Wee1 Disruption in Pancreatic Cancer Cell Cycle (Georgetown, Tex.). 2016 | Pubmed ID: 26890070 Automated Inference Procedure for the Determination of Cell Growth Parameters Physical Review. E. Jan, 2016 | Pubmed ID: 26871096 Functional Genomic Landscape of Human Breast Cancer Drivers, Vulnerabilities, and Resistance Cell. Jan, 2016 | Pubmed ID: 26771497 SMN and Symmetric Arginine Dimethylation of RNA Polymerase II C-terminal Domain Control Termination Nature. Jan, 2016 | Pubmed ID: 26700805 Identification of RNF168 As a PML Nuclear Body Regulator Journal of Cell Science. Feb, 2016 | Pubmed ID: 26675234 Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy Between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells Cell Reports. Dec, 2015 | Pubmed ID: 26673326 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface Cell. Dec, 2015 | Pubmed ID: 26638075 High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities Cell. Dec, 2015 | Pubmed ID: 26627737 Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint Genetics. Nov, 2015 | Pubmed ID: 26354767 Genome-wide RNAi Analysis Reveals That Simultaneous Inhibition of Specific Mevalonate Pathway Genes Potentiates Tumor Cell Death Oncotarget. Sep, 2015 | Pubmed ID: 26353928 Selection of Recombinant Anti-SH3 Domain Antibodies by High-throughput Phage Display Protein Science : a Publication of the Protein Society. Nov, 2015 | Pubmed ID: 26332758 Identification of P450 Oxidoreductase As a Major Determinant of Sensitivity to Hypoxia-Activated Prodrugs Cancer Research. Oct, 2015 | Pubmed ID: 26297733 A High Through-put Platform for Recombinant Antibodies to Folded Proteins Molecular & Cellular Proteomics : MCP. Oct, 2015 | Pubmed ID: 26290498 Extensive Mapping of an Innate Immune Network with CRISPR Molecular Systems Biology. Jul, 2015 | Pubmed ID: 26208794 Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes Cell. Jul, 2015 | Pubmed ID: 26186192 INNATE IMMUNITY. Cytosolic Detection of the Bacterial Metabolite HBP Activates TIFA-dependent Innate Immunity Science (New York, N.Y.). Jun, 2015 | Pubmed ID: 26068852 Inhibition of the Mitochondrial Protease ClpP As a Therapeutic Strategy for Human Acute Myeloid Leukemia Cancer Cell. Jun, 2015 | Pubmed ID: 26058080 CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces Cerevisiae G3 (Bethesda, Md.). Apr, 2015 | Pubmed ID: 26048563 Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis Cell. Jun, 2015 | Pubmed ID: 26046442 RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR Molecular & Cellular Proteomics : MCP. Jun, 2015 | Pubmed ID: 25825526 Coiled-coil Domain Containing 68 (CCDC68) Demonstrates a Tumor-suppressive Role in Pancreatic Ductal Adenocarcinoma Oncogene. Aug, 2015 | Pubmed ID: 25381825 Differential Regulation of FGFR3 by PTPN1 and PTPN2 Proteomics. Jan, 2015 | Pubmed ID: 25311528 A Systematic Approach to Identify Novel Cancer Drug Targets Using Machine Learning, Inhibitor Design and High-throughput Screening Genome Medicine. 2014 | Pubmed ID: 25165489 Measuring Error Rates in Genomic Perturbation Screens: Gold Standards for Human Functional Genomics Molecular Systems Biology. Jul, 2014 | Pubmed ID: 24987113 A Genome Scale Overexpression Screen to Reveal Drug Activity in Human Cells Genome Medicine. 2014 | Pubmed ID: 24944581 The Mammalian-membrane Two-hybrid Assay (MaMTH) for Probing Membrane-protein Interactions in Human Cells Nature Methods. May, 2014 | Pubmed ID: 24658140 CD133-targeted Niche-dependent Therapy in Cancer: a Multipronged Approach The American Journal of Pathology. May, 2014 | Pubmed ID: 24589338 Post-translational Regulation of CD133 by ATase1/ATase2-mediated Lysine Acetylation Journal of Molecular Biology. May, 2014 | Pubmed ID: 24556617 Large-scale Interaction Profiling of PDZ Domains Through Proteomic Peptide-phage Display Using Human and Viral Phage Peptidomes Proceedings of the National Academy of Sciences of the United States of America. Feb, 2014 | Pubmed ID: 24550280 The RhoGEF GEF-H1 is Required for Oncogenic RAS Signaling Via KSR-1 Cancer Cell. Feb, 2014 | Pubmed ID: 24525234 ShRNA Kinome Screen Identifies TBK1 As a Therapeutic Target for HER2+ Breast Cancer Cancer Research. Apr, 2014 | Pubmed ID: 24487029 A Negative Genetic Interaction Map in Isogenic Cancer Cell Lines Reveals Cancer Cell Vulnerabilities Molecular Systems Biology. Oct, 2013 | Pubmed ID: 24104479 Targeting HER2+ Breast Cancer Cells: Lysosomal Accumulation of Anti-HER2 Antibodies is Influenced by Antibody Binding Site and Conjugation to Polymeric Nanoparticles Journal of Controlled Release : Official Journal of the Controlled Release Society. Dec, 2013 | Pubmed ID: 23880472 Integrated Genomic, Transcriptomic, and RNA-interference Analysis of Genes in Somatic Copy Number Gains in Pancreatic Ductal Adenocarcinoma Pancreas. Aug, 2013 | Pubmed ID: 23851435 Miniature Short Hairpin RNA Screens to Characterize Antiproliferative Drugs G3 (Bethesda, Md.). Aug, 2013 | Pubmed ID: 23797109 RNA Interference Screens to Uncover Membrane Protein Biology Briefings in Functional Genomics. Sep, 2013 | Pubmed ID: 23793263 MBNL Proteins Repress ES-cell-specific Alternative Splicing and Reprogramming Nature. Jun, 2013 | Pubmed ID: 23739326 A Strategy for Modulation of Enzymes in the Ubiquitin System Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287719 Regulation of CD133 by HDAC6 Promotes β-catenin Signaling to Suppress Cancer Cell Differentiation Cell Reports. Oct, 2012 | Pubmed ID: 23084749 Suppression of Cancer Progression by MGAT1 ShRNA Knockdown PloS One. 2012 | Pubmed ID: 22957033 Dissecting DNA Damage Response Pathways by Analysing Protein Localization and Abundance Changes During DNA Replication Stress Nature Cell Biology. Sep, 2012 | Pubmed ID: 22842922 Developmentally Programmed Nuclear Destruction During Yeast Gametogenesis Developmental Cell. Jul, 2012 | Pubmed ID: 22727375 A Versatile Lentiviral Expression System to Identify Mammalian Protein-protein Interactions Methods (San Diego, Calif.). Aug, 2012 | Pubmed ID: 22713554 Essential Gene Profiles in Breast, Pancreatic, and Ovarian Cancer Cells Cancer Discovery. Feb, 2012 | Pubmed ID: 22585861 The Mixed Lineage Leukemia (MLL) Fusion-associated Gene AF4 Promotes CD133 Transcription Cancer Research. Apr, 2012 | Pubmed ID: 22337994 COLT-Cancer: Functional Genetic Screening Resource for Essential Genes in Human Cancer Cell Lines Nucleic Acids Research. Jan, 2012 | Pubmed ID: 22102578 Control of the RNA Polymerase II Phosphorylation State in Promoter Regions by CTD Interaction Domain-containing Proteins RPRD1A and RPRD1B Transcription. Sep-Oct, 2011 | Pubmed ID: 22231121 Inhibition of Mitochondrial Translation As a Therapeutic Strategy for Human Acute Myeloid Leukemia Cancer Cell. Nov, 2011 | Pubmed ID: 22094260 CD133 Protein N-glycosylation Processing Contributes to Cell Surface Recognition of the Primitive Cell Marker AC133 Epitope The Journal of Biological Chemistry. Nov, 2011 | Pubmed ID: 21937449 SnapShot: High-throughput Sequencing Applications Cell. Sep, 2011 | Pubmed ID: 21925324 Pooled Lentiviral ShRNA Screening for Functional Genomics in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21877282 A Comprehensive Platform for Highly Multiplexed Mammalian Functional Genetic Screens BMC Genomics. 2011 | Pubmed ID: 21548937 SATB2 Augments ΔNp63α in Head and Neck Squamous Cell Carcinoma EMBO Reports. Oct, 2010 | Pubmed ID: 20829881 Selective Targeting of Neuroblastoma Tumour-initiating Cells by Compounds Identified in Stem Cell-based Small Molecule Screens EMBO Molecular Medicine. Sep, 2010 | Pubmed ID: 20721990 A Lentiviral Functional Proteomics Approach Identifies Chromatin Remodeling Complexes Important for the Induction of Pluripotency Molecular & Cellular Proteomics : MCP. May, 2010 | Pubmed ID: 20305087 The Ubiquitin-activating Enzyme E1 As a Therapeutic Target for the Treatment of Leukemia and Multiple Myeloma Blood. Mar, 2010 | Pubmed ID: 20075161 Dual Regulation by Pairs of Cyclin-dependent Protein Kinases and Histone Deacetylases Controls G1 Transcription in Budding Yeast PLoS Biology. Sep, 2009 | Pubmed ID: 19823668 A Role for the TGFbeta-Par6 Polarity Pathway in Breast Cancer Progression Proceedings of the National Academy of Sciences of the United States of America. Aug, 2009 | Pubmed ID: 19667198 Exploration of Essential Gene Functions Via Titratable Promoter Alleles Cell. Jul, 2004 | Pubmed ID: 15242642 Late-G1 Cyclin-CDK Activity is Essential for Control of Cell Morphogenesis in Budding Yeast Nature Cell Biology. Jan, 2004 | Pubmed ID: 14688790 Ac'septin' a Signal: Kinase Regulation by Septins Developmental Cell. Oct, 2003 | Pubmed ID: 14536051 Dissection of a Complex Phenotype by Functional Genomics Reveals Roles for the Yeast Cyclin-dependent Protein Kinase Pho85 in Stress Adaptation and Cell Integrity Molecular and Cellular Biology. Jul, 2002 | Pubmed ID: 12077337 Transcriptional Coregulation by the Cell Integrity Mitogen-activated Protein Kinase Slt2 and the Cell Cycle Regulator Swi4 Molecular and Cellular Biology. Oct, 2001 | Pubmed ID: 11533240 Mammalian Cdk5 is a Functional Homologue of the Budding Yeast Pho85 Cyclin-dependent Protein Kinase Proceedings of the National Academy of Sciences of the United States of America. Dec, 1999 | Pubmed ID: 10588725 Cyclin Partners Determine Pho85 Protein Kinase Substrate Specificity in Vitro and in Vivo: Control of Glycogen Biosynthesis by Pcl8 and Pcl10 Molecular and Cellular Biology. Jun, 1998 | Pubmed ID: 9584169 Memeli Hücrelerinde Havuzlu CRISPR Tabanlı Genetik Ekranlar Katherine Chan*1, Amy Hin Yan Tong*1, Kevin R Brown1, Patricia Mero1, Jason Moffat1,2,3 1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto JoVE 59780 Genetics
Memeli Hücrelerinde Havuzlu CRISPR Tabanlı Genetik Ekranlar Katherine Chan*1, Amy Hin Yan Tong*1, Kevin R Brown1, Patricia Mero1, Jason Moffat1,2,3 1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto JoVE 59780 Genetics