Joseph A. Trapani Cancer Immunology Program Peter MacCallum Cancer Centre Biography Publications Institution JoVE Articles Joseph A. Trapani has not added a biography. If you are Joseph A. Trapani and would like to personalize this page please email our Author Liaison for assistance. Publications TRAIL(+) NK Cells Control CD4(+) T Cell Responses During Chronic Viral Infection to Limit Autoimmunity Immunity. Oct, 2014 | Pubmed ID: 25367576 A Method for Detecting Intracellular Perforin in Mouse Lymphocytes Journal of Immunology (Baltimore, Md. : 1950). Dec, 2014 | Pubmed ID: 25348626 Granzyme H is a Novel Protease Expressed by Human Mast Cells International Archives of Allergy and Immunology. 2014 | Pubmed ID: 25342632 The Perforin Pore Facilitates the Delivery of Cationic Cargos The Journal of Biological Chemistry. Mar, 2014 | Pubmed ID: 24558045 B-CLL Cells Acquire APC- and CTL-like Phenotypic Characteristics After Stimulation with CpG ODN and IL-21 International Immunology. Jul, 2014 | Pubmed ID: 24497611 Perforinopathy: a Spectrum of Human Immune Disease Caused by Defective Perforin Delivery or Function Frontiers in Immunology. 2013 | Pubmed ID: 24376445 Exploration of a Series of 5-arylidene-2-thioxoimidazolidin-4-ones As Inhibitors of the Cytolytic Protein Perforin Journal of Medicinal Chemistry. Dec, 2013 | Pubmed ID: 24195776 Defining the Interaction of Perforin with Calcium and the Phospholipid Membrane The Biochemical Journal. Dec, 2013 | Pubmed ID: 24070258 Rapid and Unidirectional Perforin Pore Delivery at the Cytotoxic Immune Synapse Journal of Immunology (Baltimore, Md. : 1950). Sep, 2013 | Pubmed ID: 23885110 Persistence and Efficacy of Second Generation CAR T Cell Against the LeY Antigen in Acute Myeloid Leukemia Molecular Therapy : the Journal of the American Society of Gene Therapy. Nov, 2013 | Pubmed ID: 23831595 Human Perforin Mutations and Susceptibility to Multiple Primary Cancers Oncoimmunology. Apr, 2013 | Pubmed ID: 23734337 Cloning and Characterising an Unusual Perforin from Chicken (Gallus Gallus) Developmental and Comparative Immunology. Oct, 2013 | Pubmed ID: 23680640 Perforin Forms Transient Pores on the Target Cell Plasma Membrane to Facilitate Rapid Access of Granzymes During Killer Cell Attack Blood. Apr, 2013 | Pubmed ID: 23377437 Surprisingly Variable "dangers, Toils, and Snares" Faced by Humans and Mice Blood. Jan, 2013 | Pubmed ID: 23349371 Granzyme B is Dispensable in the Development of Diabetes in Non-obese Diabetic Mice PloS One. 2012 | Pubmed ID: 22792290 A Novel Role for Granzymes in Anti-tumor Immunity Oncoimmunology. Mar, 2012 | Pubmed ID: 22720248 Protecting a Serial Killer: Pathways for Perforin Trafficking and Self-defence Ensure Sequential Target Cell Death Trends in Immunology. Aug, 2012 | Pubmed ID: 22608996 Perforin Evolved from a Gene Duplication of MPEG1, Followed by a Complex Pattern of Gene Gain and Loss Within Euteleostomi BMC Evolutionary Biology. 2012 | Pubmed ID: 22551122 Activated Mouse B Cells Lack Expression of Granzyme B Journal of Immunology (Baltimore, Md. : 1950). Apr, 2012 | Pubmed ID: 22427643 Inhibition of the Pore-forming Protein Perforin by a Series of Aryl-substituted Isobenzofuran-1(3H)-ones Bioorganic & Medicinal Chemistry. Feb, 2012 | Pubmed ID: 22244072 Fatal Immune Dysregulation Due to a Gain of Glycosylation Mutation in Lymphocyte Perforin Blood. Feb, 2012 | Pubmed ID: 22186995 A Critical Role for Granzymes in Antigen Cross-presentation Through Regulating Phagocytosis of Killed Tumor Cells Journal of Immunology (Baltimore, Md. : 1950). Aug, 2011 | Pubmed ID: 21709155 Inhibition of the Cellular Function of Perforin by 1-amino-2,4-dicyanopyrido[1,2-a]benzimidazoles Bioorganic & Medicinal Chemistry. Jul, 2011 | Pubmed ID: 21664824 Protection from Endogenous Perforin: Glycans and the C Terminus Regulate Exocytic Trafficking in Cytotoxic Lymphocytes Immunity. Jun, 2011 | Pubmed ID: 21658975 Homeostatic Defects in Interleukin 18-deficient Mice Contribute to Protection Against the Lethal Effects of Endotoxin Immunology and Cell Biology. Aug, 2011 | Pubmed ID: 21263463 The Immunostimulatory Effect of Lenalidomide on NK-cell Function is Profoundly Inhibited by Concurrent Dexamethasone Therapy Blood. Feb, 2011 | Pubmed ID: 20978269 Cathepsin C Limits Acute Viral Infection Independently of NK Cell and CD8+ T-cell Cytolytic Function Immunology and Cell Biology. May, 2011 | Pubmed ID: 20975734 Tumor Ablation by Gene-modified T Cells in the Absence of Autoimmunity Cancer Research. Dec, 2010 | Pubmed ID: 21098715 The Structural Basis for Membrane Binding and Pore Formation by Lymphocyte Perforin Nature. Nov, 2010 | Pubmed ID: 21037563 Proteases in Lymphocyte Killer Function: Redundancy, Polymorphism and Questions Remaining Biological Chemistry. Aug, 2010 | Pubmed ID: 20731542 A Role for Granzyme M in TLR4-driven Inflammation and Endotoxicosis Journal of Immunology (Baltimore, Md. : 1950). Aug, 2010 | Pubmed ID: 20585036 Functional Dissection of the Granzyme Family: Cell Death and Inflammation Immunological Reviews. May, 2010 | Pubmed ID: 20536556 Perforin: Structure, Function, and Role in Human Immunopathology Immunological Reviews. May, 2010 | Pubmed ID: 20536554 Human and Mouse Perforin Are Processed in Part Through Cleavage by the Lysosomal Cysteine Proteinase Cathepsin L Immunology. Oct, 2010 | Pubmed ID: 20497254 Functional Crosstalk Between Type I and II Interferon Through the Regulated Expression of STAT1 PLoS Biology. 2010 | Pubmed ID: 20436908 Cathepsin H is an Additional Convertase of Pro-granzyme B The Journal of Biological Chemistry. Jul, 2010 | Pubmed ID: 20435891 Incentives: Encouraging Adventurous Ideas Science (New York, N.Y.). Jan, 2010 | Pubmed ID: 20056875 The Battlefield of Perforin/granzyme Cell Death Pathways Journal of Leukocyte Biology. Feb, 2010 | Pubmed ID: 19915166 Endolysosomal Proteases and Their Inhibitors in Immunity Nature Reviews. Immunology. Dec, 2009 | Pubmed ID: 19935806 Proteolytic Activation of the Cytotoxic Phenotype During Human NK Cell Development Journal of Immunology (Baltimore, Md. : 1950). Jul, 2009 | Pubmed ID: 19570824 Cutting Edge: Rapid and Efficient in Vivo Cytotoxicity by Cytotoxic T Cells is Independent of Granzymes A and B Journal of Immunology (Baltimore, Md. : 1950). Jul, 2009 | Pubmed ID: 19525394 Temperature Sensitivity of Human Perforin Mutants Unmasks Subtotal Loss of Cytotoxicity, Delayed FHL, and a Predisposition to Cancer Proceedings of the National Academy of Sciences of the United States of America. Jun, 2009 | Pubmed ID: 19487666 The Molecular Basis for Perforin Oligomerization and Transmembrane Pore Assembly Immunity. May, 2009 | Pubmed ID: 19446473 The Lewis-Y Carbohydrate Antigen is Expressed by Many Human Tumors and Can Serve As a Target for Genetically Redirected T Cells Despite the Presence of Soluble Antigen in Serum Journal of Immunotherapy (Hagerstown, Md. : 1997). Apr, 2009 | Pubmed ID: 19242371 Perforin-mediated Suppression of B-cell Lymphoma Proceedings of the National Academy of Sciences of the United States of America. Feb, 2009 | Pubmed ID: 19196996 Dihydrofuro[3,4-c]pyridinones As Inhibitors of the Cytolytic Effects of the Pore-forming Glycoprotein Perforin Journal of Medicinal Chemistry. Dec, 2008 | Pubmed ID: 19007200 A Renaissance in Understanding the Multiple and Diverse Functions of Granzymes? Immunity. Nov, 2008 | Pubmed ID: 19006688 Granzyme K Expressing Cytotoxic T Lymphocytes Protects Against Influenza Virus in Granzyme AB-/- Mice Viral Immunology. Sep, 2008 | Pubmed ID: 18788942 The MACPF/CDC Family of Pore-forming Toxins Cellular Microbiology. Sep, 2008 | Pubmed ID: 18564372 The Role of P202 in Regulating Hematopoietic Cell Proliferation and Differentiation Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research. Jan, 2008 | Pubmed ID: 18370867 Measuring Cell Death Mediated by Cytotoxic Lymphocytes or Their Granule Effector Molecules Methods (San Diego, Calif.). Mar, 2008 | Pubmed ID: 18314055 Cloning and Characterisation of Ifi206: a New Murine HIN-200 Family Member Journal of Cellular Biochemistry. Mar, 2008 | Pubmed ID: 17786933 Sustained Antigen-specific Antitumor Recall Response Mediated by Gene-modified CD4+ T Helper-1 and CD8+ T Cells Cancer Research. Dec, 2007 | Pubmed ID: 18056471 H is for Helper: Granzyme H Helps Granzyme B Kill Adenovirus-infected Cells Trends in Immunology. Sep, 2007 | Pubmed ID: 17766182 A Common Fold Mediates Vertebrate Defense and Bacterial Attack Science (New York, N.Y.). Sep, 2007 | Pubmed ID: 17717151 Infective, Neoplastic, and Homeostatic Sequelae of the Loss of Perforin Function in Humans Advances in Experimental Medicine and Biology. 2007 | Pubmed ID: 17713010 The Granzyme B Gene is Highly Polymorphic in Wild Mice but Essentially Invariant in Common Inbred Laboratory Strains Tissue Antigens. Sep, 2007 | Pubmed ID: 17661907 Perforin Activity and Immune Homeostasis: the Common A91V Polymorphism in Perforin Results in Both Presynaptic and Postsynaptic Defects in Function Blood. Aug, 2007 | Pubmed ID: 17475905 The Complex Issue of Regulating Perforin Expression Trends in Immunology. Jun, 2007 | Pubmed ID: 17466595 Apoptosis Induced by the Lymphocyte Effector Molecule Perforin Current Opinion in Immunology. Jun, 2007 | Pubmed ID: 17442557 Residual Active Granzyme B in Cathepsin C-null Lymphocytes is Sufficient for Perforin-dependent Target Cell Apoptosis The Journal of Cell Biology. Feb, 2007 | Pubmed ID: 17283185 A Novel C-Jun-dependent Signal Transduction Pathway Necessary for the Transcriptional Activation of Interferon Gamma Response Genes The Journal of Biological Chemistry. Jan, 2007 | Pubmed ID: 17105733 Perforin-mediated Target-cell Death and Immune Homeostasis Nature Reviews. Immunology. Dec, 2006 | Pubmed ID: 17124515 Ligation of the Cell Surface Receptor, CD46, Alters T Cell Polarity and Response to Antigen Presentation Proceedings of the National Academy of Sciences of the United States of America. Dec, 2006 | Pubmed ID: 17116876 The Major Human and Mouse Granzymes Are Structurally and Functionally Divergent The Journal of Cell Biology. Nov, 2006 | Pubmed ID: 17116752 Cytotoxic T-cells from T-cell Receptor Transgenic NOD8.3 Mice Destroy Beta-cells Via the Perforin and Fas Pathways Diabetes. Sep, 2006 | Pubmed ID: 16936188 Cytotoxic T Lymphocytes from Cathepsin B-deficient Mice Survive Normally in Vitro and in Vivo After Encountering and Killing Target Cells The Journal of Biological Chemistry. Oct, 2006 | Pubmed ID: 16914553 Granzyme B-mediated Death of Pancreatic Beta-cells Requires the Proapoptotic BH3-only Molecule Bid Diabetes. Aug, 2006 | Pubmed ID: 16873683 Cytotoxic T Lymphocyte-induced Killing in the Absence of Granzymes A and B is Unique and Distinct from Both Apoptosis and Perforin-dependent Lysis The Journal of Cell Biology. Apr, 2006 | Pubmed ID: 16606695 Perforin and Fas Induced by IFNgamma and TNFalpha Mediate Beta Cell Death by OT-I CTL International Immunology. Jun, 2006 | Pubmed ID: 16574667 A Critical Role for Granzyme B, in Addition to Perforin and TNFalpha, in Alloreactive CTL-induced Mouse Pancreatic Beta Cell Death Transplantation. Jan, 2006 | Pubmed ID: 16436955 Role of Bid-induced Mitochondrial Outer Membrane Permeabilization in Granzyme B-induced Apoptosis Immunology and Cell Biology. Feb, 2006 | Pubmed ID: 16405654 Addressing the Mysteries of Perforin Function Immunology and Cell Biology. Feb, 2006 | Pubmed ID: 16405653 Report on Apoptosis and Immunity, 31 May-3 June 2005, Palm Cove, Queensland, Australia Immunology and Cell Biology. Feb, 2006 | Pubmed ID: 16405648 Adoptive Transfer of T Cells Modified with a Humanized Chimeric Receptor Gene Inhibits Growth of Lewis-Y-expressing Tumors in Mice Proceedings of the National Academy of Sciences of the United States of America. Dec, 2005 | Pubmed ID: 16365285 The Dual Adverse Effects of TGF-beta Secretion on Tumor Progression Cancer Cell. Nov, 2005 | Pubmed ID: 16286241 Cationic Sites on Granzyme B Contribute to Cytotoxicity by Promoting Its Uptake into Target Cells Molecular and Cellular Biology. Sep, 2005 | Pubmed ID: 16107729 Adoptive Transfer of Gene-engineered CD4+ Helper T Cells Induces Potent Primary and Secondary Tumor Rejection Blood. Nov, 2005 | Pubmed ID: 16030195 Extracellular Matrix Remodeling by Human Granzyme B Via Cleavage of Vitronectin, Fibronectin, and Laminin The Journal of Biological Chemistry. Jun, 2005 | Pubmed ID: 15843372 Interaction of the Nuclear Localizing Cytolytic Granule Serine Protease Granzyme B with Importin Alpha or Beta: Modulation by the Serpin Inhibitor PI-9 Journal of Cellular Biochemistry. Jun, 2005 | Pubmed ID: 15791691 A Functional Analysis of the Putative Polymorphisms A91V and N252S and 22 Missense Perforin Mutations Associated with Familial Hemophagocytic Lymphohistiocytosis Blood. Jun, 2005 | Pubmed ID: 15755897 Biochemical and Growth Regulatory Activities of the HIN-200 Family Member and Putative Tumor Suppressor Protein, AIM2 Biochemical and Biophysical Research Communications. Jan, 2005 | Pubmed ID: 15582594 Calcium-dependent Plasma Membrane Binding and Cell Lysis by Perforin Are Mediated Through Its C2 Domain: A Critical Role for Aspartate Residues 429, 435, 483, and 485 but Not 491 The Journal of Biological Chemistry. Mar, 2005 | Pubmed ID: 15576364 A Central Role for Bid in Granzyme B-induced Apoptosis The Journal of Biological Chemistry. Feb, 2005 | Pubmed ID: 15574417 The Functional Basis for Hemophagocytic Lymphohistiocytosis in a Patient with Co-inherited Missense Mutations in the Perforin (PFN1) Gene The Journal of Experimental Medicine. Sep, 2004 | Pubmed ID: 15365097 Cytotoxic Lymphocytes; Instigators of Dramatic Target Cell Death Biochemical Pharmacology. Sep, 2004 | Pubmed ID: 15313398 Gene-engineered T Cells As a Superior Adjuvant Therapy for Metastatic Cancer Journal of Immunology (Baltimore, Md. : 1950). Aug, 2004 | Pubmed ID: 15265951 Cytotoxic Activity of the Lymphocyte Toxin Granzyme B Microbes and Infection / Institut Pasteur. Jul, 2004 | Pubmed ID: 15207822 Assaying Cytochrome C Translocation During Apoptosis Methods in Molecular Biology (Clifton, N.J.). 2004 | Pubmed ID: 15173625 Discordant Regulation of Granzyme H and Granzyme B Expression in Human Lymphocytes The Journal of Biological Chemistry. Jun, 2004 | Pubmed ID: 15069086 A Functional Role for CD28 Costimulation in Tumor Recognition by Single-chain Receptor-modified T Cells Cancer Gene Therapy. May, 2004 | Pubmed ID: 15060573 Granzyme M Mediates a Novel Form of Perforin-dependent Cell Death The Journal of Biological Chemistry. May, 2004 | Pubmed ID: 15028722 Granzyme B Encoded by the Commonly Occurring Human RAH Allele Retains Pro-apoptotic Activity The Journal of Biological Chemistry. Apr, 2004 | Pubmed ID: 14752093 HScrib is a Functional Homologue of the Drosophila Tumour Suppressor Scribble Oncogene. Dec, 2003 | Pubmed ID: 14681682 Granzyme B: Pro-apoptotic, Antiviral and Antitumor Functions Current Opinion in Immunology. Oct, 2003 | Pubmed ID: 14499262 Novel Mechanisms of Apoptosis Induced by Histone Deacetylase Inhibitors Cancer Research. Aug, 2003 | Pubmed ID: 12907619 Cutting Edge: Granzymes A and B Are Not Essential for Perforin-mediated Tumor Rejection Journal of Immunology (Baltimore, Md. : 1950). Jul, 2003 | Pubmed ID: 12847210 Purification of Natural Killer Cell Cytotoxic Granules for Assaying Target Cell Apoptosis Journal of Immunological Methods. May, 2003 | Pubmed ID: 12738359 Caspase Activation by Granzyme B is Indirect, and Caspase Autoprocessing Requires the Release of Proapoptotic Mitochondrial Factors Immunity. Mar, 2003 | Pubmed ID: 12648450 Expression of IFI 16 in Epithelial Cells and Lymphoid Tissues Histochemistry and Cell Biology. Jan, 2003 | Pubmed ID: 12548405 A Clathrin/dynamin- and Mannose-6-phosphate Receptor-independent Pathway for Granzyme B-induced Cell Death The Journal of Cell Biology. Jan, 2003 | Pubmed ID: 12538642 Rejection of Syngeneic Colon Carcinoma by CTLs Expressing Single-chain Antibody Receptors Codelivering CD28 Costimulation Journal of Immunology (Baltimore, Md. : 1950). Nov, 2002 | Pubmed ID: 12421958 Single-chain Antigen Recognition Receptors That Costimulate Potent Rejection of Established Experimental Tumors Blood. Nov, 2002 | Pubmed ID: 12384413 Functional Significance of the Perforin/granzyme Cell Death Pathway Nature Reviews. Immunology. Oct, 2002 | Pubmed ID: 12360212 Tumor-mediated Apoptosis of Cancer-specific T Lymphocytes--reversing the "kiss of Death"? Cancer Cell. Sep, 2002 | Pubmed ID: 12242147 Suppression of Lymphoma and Epithelial Malignancies Effected by Interferon Gamma The Journal of Experimental Medicine. Jul, 2002 | Pubmed ID: 12093877 Functional Role of Phosphatidylinositol 3-kinase in Direct Tumor Lysis by Human Natural Killer Cells Immunobiology. Mar, 2002 | Pubmed ID: 11999346 Cytotoxic Cell Granule-mediated Apoptosis: Perforin Delivers Granzyme B-serglycin Complexes into Target Cells Without Plasma Membrane Pore Formation Immunity. Mar, 2002 | Pubmed ID: 11911826 Een colorimetrische test waarmee specifiek Granzyme B proteolytische activiteit: Hydrolyse van Boc-Ala-Ala-Asp-S-Bzl Magdalena Hagn1, Vivien R. Sutton1, Joseph A. Trapani1 1Cancer Immunology Program, Peter MacCallum Cancer Centre Chemistry
Een colorimetrische test waarmee specifiek Granzyme B proteolytische activiteit: Hydrolyse van Boc-Ala-Ala-Asp-S-Bzl Magdalena Hagn1, Vivien R. Sutton1, Joseph A. Trapani1 1Cancer Immunology Program, Peter MacCallum Cancer Centre Chemistry