Mark A. Behlke Integrated DNA Technologies, Inc. Biography Publications Institution JoVE Articles Mark A. Behlke has not added a biography. If you are Mark A. Behlke and would like to personalize this page please email our Author Liaison for assistance. Publications Platelet Activating Factor Receptor Activation Improves SiRNA Uptake and RNAi Responses in Well-differentiated Airway Epithelia Molecular Therapy. Nucleic Acids. 2014 | Pubmed ID: 25025465 Noninvasive Imaging of Staphylococcus Aureus Infections with a Nuclease-activated Probe Nature Medicine. Mar, 2014 | Pubmed ID: 24487433 Imaging the Directed Transport of Single Engineered RNA Transcripts in Real-time Using Ratiometric Bimolecular Beacons PloS One. 2014 | Pubmed ID: 24454933 Inosine-mediated Modulation of RNA Sensing by Toll-like Receptor 7 (TLR7) and TLR8 Journal of Virology. Jan, 2014 | Pubmed ID: 24227841 In Vivo Comparison of Local Versus Systemic Delivery of Immunostimulating SiRNA in HPV-driven Tumours Immunology and Cell Biology. Feb, 2014 | Pubmed ID: 24217808 Knockdown of β-catenin with Dicer-substrate SiRNAs Reduces Liver Tumor Burden in Vivo Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2014 | Pubmed ID: 24089139 Correction: SNP Detection in MRNA in Living Cells Using Allele Specific FRET Probes PloS One. 2013 | Pubmed ID: 24244230 SNP Detection in MRNA in Living Cells Using Allele Specific FRET Probes PloS One. 2013 | Pubmed ID: 24039756 Improved Performance of Anti-miRNA Oligonucleotides Using a Novel Non-Nucleotide Modifier Molecular Therapy. Nucleic Acids. 2013 | Pubmed ID: 23982190 Quantitative Assessment of Ratiometric Bimolecular Beacons As a Tool for Imaging Single Engineered RNA Transcripts and Measuring Gene Expression in Living Cells Nucleic Acids Research. Aug, 2013 | Pubmed ID: 23814183 Spinal NTS2 Receptor Activation Reverses Signs of Neuropathic Pain FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Sep, 2013 | Pubmed ID: 23756650 U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo Molecular Therapy. Nucleic Acids. 2013 | Pubmed ID: 23673539 Post-transcriptional Regulation of Cystic Fibrosis Transmembrane Conductance Regulator Expression and Function by MicroRNAs American Journal of Respiratory Cell and Molecular Biology. Oct, 2013 | Pubmed ID: 23646886 Efficient Delivery of RNA Interference Oligonucleotides to Polarized Airway Epithelia in Vitro American Journal of Physiology. Lung Cellular and Molecular Physiology. Jul, 2013 | Pubmed ID: 23624792 Altered Purkinje Cell MiRNA Expression and SCA1 Pathogenesis Neurobiology of Disease. Jun, 2013 | Pubmed ID: 23376683 Targeting IκB Proteins for HIV Latency Activation: the Role of Individual IκB and NF-κB Proteins Journal of Virology. Apr, 2013 | Pubmed ID: 23365428 In Vivo SELEX for Identification of Brain-penetrating Aptamers Molecular Therapy. Nucleic Acids. 2013 | Pubmed ID: 23299833 Manipulation of Cell Physiology Enables Gene Silencing in Well-differentiated Airway Epithelia Molecular Therapy. Nucleic Acids. 2012 | Pubmed ID: 23344182 Deep Sequencing Analyses of DsiRNAs Reveal the Influence of 3' Terminal Overhangs on Dicing Polarity, Strand Selectivity, and RNA Editing of SiRNAs Molecular Therapy. Nucleic Acids. 2012 | Pubmed ID: 23343928 Alternative RISC Assembly: Binding and Repression of MicroRNA-mRNA Duplexes by Human Ago Proteins RNA (New York, N.Y.). Nov, 2012 | Pubmed ID: 23019594 Rapid Identification of Cell-specific, Internalizing RNA Aptamers with Bioinformatics Analyses of a Cell-based Aptamer Selection PloS One. 2012 | Pubmed ID: 22962591 RNA Inhibition Highlights Cyclin D1 As a Potential Therapeutic Target for Mantle Cell Lymphoma PloS One. 2012 | Pubmed ID: 22905260 A MicroRNA Network Regulates Expression and Biosynthesis of Wild-type and DeltaF508 Mutant Cystic Fibrosis Transmembrane Conductance Regulator Proceedings of the National Academy of Sciences of the United States of America. Aug, 2012 | Pubmed ID: 22853952 Right- and Left-loop Short ShRNAs Have Distinct and Unusual Mechanisms of Gene Silencing Nucleic Acids Research. Oct, 2012 | Pubmed ID: 22810205 Pancreas-enriched MiRNA Refines Endocrine Cell Differentiation Development (Cambridge, England). Aug, 2012 | Pubmed ID: 22764048 RNA Aptamer-based Functional Ligands of the Neurotrophin Receptor, TrkB Molecular Pharmacology. Oct, 2012 | Pubmed ID: 22752556 A MiR-19 Regulon That Controls NF-κB Signaling Nucleic Acids Research. Sep, 2012 | Pubmed ID: 22684508 Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization ISRN Pharmaceutics. 2012 | Pubmed ID: 22474606 Degradation of Nuclease-stabilized RNA Oligonucleotides in Mycoplasma-contaminated Cell Culture Media Nucleic Acid Therapeutics. Feb, 2012 | Pubmed ID: 22229275 Progress Toward in Vivo Use of SiRNAs-II Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2012 | Pubmed ID: 22186795 Protection Against TNFα-dependent Liver Toxicity by Intraperitoneal Liposome Delivered DsiRNA Targeting TNFα in Vivo Journal of Controlled Release : Official Journal of the Controlled Release Society. Jun, 2012 | Pubmed ID: 22094102 Synthesis and Characterization of Mannosylated Pegylated Polyethylenimine As a Carrier for SiRNA International Journal of Pharmaceutics. May, 2012 | Pubmed ID: 21864664 SiRNA-induced Immunostimulation Through TLR7 Promotes Antitumoral Activity Against HPV-driven Tumors in Vivo Immunology and Cell Biology. Feb, 2012 | Pubmed ID: 21423261 RNase H-dependent PCR (rhPCR): Improved Specificity and Single Nucleotide Polymorphism Detection Using Blocked Cleavable Primers BMC Biotechnology. 2011 | Pubmed ID: 21831278 Nucleotide Bias Observed with a Short SELEX RNA Aptamer Library Nucleic Acid Therapeutics. Aug, 2011 | Pubmed ID: 21793789 Small Nucleolar RNAs U32a, U33, and U35a Are Critical Mediators of Metabolic Stress Cell Metabolism. Jul, 2011 | Pubmed ID: 21723502 Analysis of MicroRNA Turnover in Mammalian Cells Following Dicer1 Ablation Nucleic Acids Research. Jul, 2011 | Pubmed ID: 21447562 MiRNAs Control Insulin Content in Pancreatic β-cells Via Downregulation of Transcriptional Repressors The EMBO Journal. Mar, 2011 | Pubmed ID: 21285947 A Role for Human Dicer in Pre-RISC Loading of SiRNAs Nucleic Acids Research. Mar, 2011 | Pubmed ID: 20972213 Intraperitoneal Administration of Chitosan/DsiRNA Nanoparticles Targeting TNFα Prevents Radiation-induced Fibrosis Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. Oct, 2010 | Pubmed ID: 20889220 Genetic Modulation of TLR8 Response Following Bacterial Phagocytosis Human Mutation. Sep, 2010 | Pubmed ID: 20652908 Ratiometric Bimolecular Beacons for the Sensitive Detection of RNA in Single Living Cells Nucleic Acids Research. Aug, 2010 | Pubmed ID: 20507905 A Direct Comparison of Anti-microRNA Oligonucleotide Potency Pharmaceutical Research. Sep, 2010 | Pubmed ID: 20424893 Rational Design of Immunostimulatory SiRNAs Molecular Therapy : the Journal of the American Society of Gene Therapy. Apr, 2010 | Pubmed ID: 20125126 Effects of Chemical Modification on the Potency, Serum Stability, and Immunostimulatory Properties of Short ShRNAs RNA (New York, N.Y.). Jan, 2010 | Pubmed ID: 19948766 Sub-cellular Trafficking and Functionality of 2'-O-methyl and 2'-O-methyl-phosphorothioate Molecular Beacons Nucleic Acids Research. Dec, 2009 | Pubmed ID: 19820111 The Beta3 Subunit of the Na+,K+-ATPase Mediates Variable Nociceptive Sensitivity in the Formalin Test Pain. Aug, 2009 | Pubmed ID: 19464798 Gene Silencing by Synthetic U1 Adaptors Nature Biotechnology. Mar, 2009 | Pubmed ID: 19219028 Chitosan/siRNA Nanoparticle-mediated TNF-alpha Knockdown in Peritoneal Macrophages for Anti-inflammatory Treatment in a Murine Arthritis Model Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2009 | Pubmed ID: 18827803 Regulation of Bacteria-induced Intercellular Adhesion Molecule-1 by CCAAT/enhancer Binding Proteins American Journal of Respiratory Cell and Molecular Biology. Feb, 2009 | Pubmed ID: 18703796 Chemical Modification of SiRNAs for in Vivo Use Oligonucleotides. Dec, 2008 | Pubmed ID: 19025401 A Role for the Dicer Helicase Domain in the Processing of Thermodynamically Unstable Hairpin RNAs Nucleic Acids Research. Nov, 2008 | Pubmed ID: 18927112 Assessing the Sensitivity of Commercially Available Fluorophores to the Intracellular Environment Analytical Chemistry. Oct, 2008 | Pubmed ID: 18700780 Chemical Modification Patterns Compatible with High Potency Dicer-substrate Small Interfering RNAs Oligonucleotides. Jun, 2008 | Pubmed ID: 18637735 Central Delivery of Dicer-substrate SiRNA: a Direct Application for Pain Research Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2008 | Pubmed ID: 18523447 Efficient Cytosolic Delivery of Molecular Beacon Conjugates and Flow Cytometric Analysis of Target RNA Nucleic Acids Research. Jul, 2008 | Pubmed ID: 18503086 Determinants of Cytokine Induction by Small Interfering RNA in Human Peripheral Blood Mononuclear Cells Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research. Apr, 2008 | Pubmed ID: 18439100 Predicting Stability of DNA Duplexes in Solutions Containing Magnesium and Monovalent Cations Biochemistry. May, 2008 | Pubmed ID: 18422348 TLR7 is Involved in Sequence-specific Sensing of Single-stranded RNAs in Human Macrophages Journal of Immunology (Baltimore, Md. : 1950). Feb, 2008 | Pubmed ID: 18250417 Advances in Molecular and Cellular Therapies for Hearing Loss Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2008 | Pubmed ID: 18223547 Avoiding False-positive Signals with Nuclease-vulnerable Molecular Beacons in Single Living Cells Nucleic Acids Research. 2007 | Pubmed ID: 17702767 Design of Active Small Interfering RNAs Current Opinion in Molecular Therapeutics. Apr, 2007 | Pubmed ID: 17458163 Rational Design and in Vitro and in Vivo Delivery of Dicer Substrate SiRNA Nature Protocols. 2006 | Pubmed ID: 17406276 Design of LNA Probes That Improve Mismatch Discrimination Nucleic Acids Research. 2006 | Pubmed ID: 16670427 A Structural Basis for Discriminating Between Self and Nonself Double-stranded RNAs in Mammalian Cells Nature Biotechnology. May, 2006 | Pubmed ID: 16648842 Stable Expression of ShRNAs in Human CD34+ Progenitor Cells Can Avoid Induction of Interferon Responses to SiRNAs in Vitro Nature Biotechnology. May, 2006 | Pubmed ID: 16648841 Characterization of Modified Antisense Oligonucleotides in Xenopus Laevis Embryos Oligonucleotides. 2006 | Pubmed ID: 16584293 Progress Towards in Vivo Use of SiRNAs Molecular Therapy : the Journal of the American Society of Gene Therapy. Apr, 2006 | Pubmed ID: 16481219 Respiratory Syncytial Virus Nonstructural Protein 2 Specifically Inhibits Type I Interferon Signal Transduction Virology. Jan, 2006 | Pubmed ID: 16216295 Functional Polarity is Introduced by Dicer Processing of Short Substrate RNAs Nucleic Acids Research. 2005 | Pubmed ID: 16049023 A Potential Role for RNA Interference in Controlling the Activity of the Human LINE-1 Retrotransposon Nucleic Acids Research. 2005 | Pubmed ID: 15701756 Effects of Fluorescent Dyes, Quenchers, and Dangling Ends on DNA Duplex Stability Biochemical and Biophysical Research Communications. Feb, 2005 | Pubmed ID: 15629139 Synthetic DsRNA Dicer Substrates Enhance RNAi Potency and Efficacy Nature Biotechnology. Feb, 2005 | Pubmed ID: 15619617 Effects of Sodium Ions on DNA Duplex Oligomers: Improved Predictions of Melting Temperatures Biochemistry. Mar, 2004 | Pubmed ID: 15035624 Thermodynamic Treatment of Oligonucleotide Duplex-simplex Equilibria Proceedings of the National Academy of Sciences of the United States of America. Dec, 2003 | Pubmed ID: 14657395 Spectroscopic Features of Dual Fluorescence/luminescence Resonance Energy-transfer Molecular Beacons Analytical Chemistry. Aug, 2003 | Pubmed ID: 14572032 Hybridization of 2'-O-methyl and 2'-deoxy Molecular Beacons to RNA and DNA Targets Nucleic Acids Research. Mar, 2003 | Pubmed ID: 12669716 Hybridization Kinetics and Thermodynamics of Molecular Beacons Nucleic Acids Research. Feb, 2003 | Pubmed ID: 12582252 Hybridization of 2'-O-methyl and 2'-deoxy Molecular Beacons to RNA and DNA Targets Nucleic Acids Research. Dec, 2002 | Pubmed ID: 12466541 Structure-function Relationships of Shared-stem and Conventional Molecular Beacons Nucleic Acids Research. Oct, 2002 | Pubmed ID: 12364599 Real-time Imaging af Single Engineered RNA-transkripter i levende celler Brug Ratiometrisk bimolekylær Beacons Yang Song*1, Xuemei Zhang*1, Lingyan Huang2, Mark A. Behlke2, Andrew Tsourkas1 1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc. JoVE 51544 Biology
Real-time Imaging af Single Engineered RNA-transkripter i levende celler Brug Ratiometrisk bimolekylær Beacons Yang Song*1, Xuemei Zhang*1, Lingyan Huang2, Mark A. Behlke2, Andrew Tsourkas1 1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc. JoVE 51544 Biology