Photini Sinnis Molecular Microbiology and Immunology Johns Hopkins School of Public Health Biography Publications Institution JoVE Articles Photini Sinnis The Sinnis Laboratory is part of the Johns Hopkins Malaria Research Institute, committed to the pursuit of basic science research that translates into solutions targeting one of the most important infectious diseases in the world. Malaria, caused by protozoan parasites of the genus Plasmodium, is transmitted to humans by infected Anopheles mosquitoes. The evolution of drug resistance by the parasite, and insecticide resistance by the mosquito, have created an urgent need for new strategies to control and ultimately eradicate this scourge. Our research focuses on the infective stage of the malaria parasite, sporozoites, which are inoculated into the skin by infected mosquitoes. Sporozoites make an impressive journey from the midgut wall of the mosquito where they emerge from oocysts, to their final destination, the mammalian liver. Using biochemical, cell biological, and genetic approaches as well as intravital imaging and proteomics, we aim to understand the molecular interactions between sporozoites and their mosquito and mammalian hosts that enable the parasite to initiate infection. Recently we have started investigation the quantitative dynamics of transmission from mosquito to mammalian host, an understudied yet important area of inquiry if we are to improve our epidemiologic models and define the minimum efficacy required of transmission blocking interventions. Publications Reply to Vanderberg, "Further Mechanisms and Locations in Which Antisporozoite Antibodies Neutralize Malaria Sporozoites" MBio. 09, 2019 | Pubmed ID: 31506316 Author Correction: A Human Monoclonal Antibody Prevents Malaria Infection by Targeting a New Site of Vulnerability on the Parasite Nature Medicine. Jan, 2019 | Pubmed ID: 30552419 Antibody-Mediated Protection Against Sporozoites Begins at the Dermal Inoculation Site MBio. 11, 2018 | Pubmed ID: 30459199 A Mosquito Salivary Gland Protein Partially Inhibits Plasmodium Sporozoite Cell Traversal and Transmission Nature Communications. 07, 2018 | Pubmed ID: 30046053 A Human Monoclonal Antibody Prevents Malaria Infection by Targeting a New Site of Vulnerability on the Parasite Nature Medicine. 05, 2018 | Pubmed ID: 29554083 Integrating Transcriptomic and Proteomic Data for Accurate Assembly and Annotation of Genomes Genome Research. 01, 2017 | Pubmed ID: 28003436 Overexpression of Plasmodium Berghei ATG8 by Liver Forms Leads to Cumulative Defects in Organelle Dynamics and to Generation of Noninfectious Merozoites MBio. 06, 2016 | Pubmed ID: 27353755 Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics PLoS Pathogens. 04, 2016 | Pubmed ID: 27128092 Palmitoyl Transferases Have Critical Roles in the Development of Mosquito and Liver Stages of Plasmodium Cellular Microbiology. Nov, 2016 | Pubmed ID: 27084458 Inhibition by Stabilization: Targeting the Plasmodium Falciparum Aldolase-TRAP Complex Malaria Journal. Aug, 2015 | Pubmed ID: 26289816 Reversible Conformational Change in the Plasmodium Falciparum Circumsporozoite Protein Masks Its Adhesion Domains Infection and Immunity. Oct, 2015 | Pubmed ID: 26169272 Proteolytic Cleavage of the Plasmodium Falciparum Circumsporozoite Protein Is a Target of Protective Antibodies The Journal of Infectious Diseases. Oct, 2015 | Pubmed ID: 25762791 A Host GPCR Signaling Network Required for the Cytolysis of Infected Cells Facilitates Release of Apicomplexan Parasites Cell Host & Microbe. Jan, 2013 | Pubmed ID: 23332153 Total and Putative Surface Proteomics of Malaria Parasite Salivary Gland Sporozoites Molecular & Cellular Proteomics : MCP. May, 2013 | Pubmed ID: 23325771 Dendritic Cells and Hepatocytes Use Distinct Pathways to Process Protective Antigen from Plasmodium in Vivo PLoS Pathogens. Mar, 2011 | Pubmed ID: 21445239 Evidence That Mutant PfCRT Facilitates the Transmission to Mosquitoes of Chloroquine-treated Plasmodium Gametocytes The Journal of Infectious Diseases. Jan, 2011 | Pubmed ID: 21288823 Plasmodium falciparum Culture de gamétocytes et infection par les moustiques par l’alimentation par membrane artificielle Abhai K Tripathi1, Godfree Mlambo1, Sachie Kanatani1, Photini Sinnis1, George Dimopoulos1 1Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University JoVE 61426 Biology
Plasmodium falciparum Culture de gamétocytes et infection par les moustiques par l’alimentation par membrane artificielle Abhai K Tripathi1, Godfree Mlambo1, Sachie Kanatani1, Photini Sinnis1, George Dimopoulos1 1Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University JoVE 61426 Biology