Robert Glaeser Molecular Biophysics and Integrative Bio-Imaging Division Lawrence Berkeley National Laboratory Biography Publications Institution JoVE Articles Robert Glaeser has not added a biography. If you are Robert Glaeser and would like to personalize this page please email our Author Liaison for assistance. Publications Overcoming Resolution Loss in Laser Phase Plate Cryo-Electron Microscopy Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. Jul, 2023 | Pubmed ID: 37613521 Challenges in Making Ideal Cryo-EM Samples Current Opinion in Structural Biology. Aug, 2023 | Pubmed ID: 37392555 Overcoming Resolution Loss Due to Thermal Magnetic Field Fluctuations from Phase Plates in Transmission Electron Microscopy Ultramicroscopy. Jul, 2023 | Pubmed ID: 37011498 Overcoming Resolution Loss Due to Thermal Magnetic Field Fluctuations from Phase Plates in Transmission Electron Microscopy BioRxiv : the Preprint Server for Biology. Feb, 2023 | Pubmed ID: 36824829 Perspective: Biochemical and Physical Constraints Associated With Preparing Thin Specimens for Single-Particle Cryo-EM Frontiers in Molecular Biosciences. 2022 | Pubmed ID: 35573724 Perspective: Emerging Strategies for Determining Atomic-resolution Structures of Macromolecular Complexes Within Cells Journal of Structural Biology. Mar, 2022 | Pubmed ID: 34915129 Conquer by Cryo-EM Without Physically Dividing Biochemical Society Transactions. Nov, 2021 | Pubmed ID: 34709401 Simple Assay for Adsorption of Proteins to the Air-water Interface Journal of Structural Biology. Dec, 2021 | Pubmed ID: 34534654 High-power Near-concentric Fabry-Perot Cavity for Phase Contrast Electron Microscopy The Review of Scientific Instruments. May, 2021 | Pubmed ID: 34243315 Minimizing Crinkling of Soft Specimens Using Holey Gold Films on Molybdenum Grids for Cryogenic Electron Microscopy Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. Aug, 2021 | Pubmed ID: 34085628 Preparing Better Samples for Cryo-Electron Microscopy: Biochemical Challenges Do Not End with Isolation and Purification Annual Review of Biochemistry. Jun, 2021 | Pubmed ID: 33556280 Defocus-dependent Thon-ring Fading Ultramicroscopy. Mar, 2021 | Pubmed ID: 33524638 Spectral DQE of the Volta Phase Plate Ultramicroscopy. Nov, 2020 | Pubmed ID: 32739753 Observation of the Relativistic Reversal of the Ponderomotive Potential Physical Review Letters. May, 2020 | Pubmed ID: 32412292 Microscale Fluid Behavior During Cryo-EM Sample Blotting Biophysical Journal. Feb, 2020 | Pubmed ID: 31952802 Laser Phase Plate for Transmission Electron Microscopy Nature Methods. Oct, 2019 | Pubmed ID: 31562475 Current Outcomes when Optimizing 'standard' Sample Preparation for Single-particle Cryo-EM Journal of Microscopy. Oct, 2019 | Pubmed ID: 31553060 How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits? Annual Review of Biophysics. May, 2019 | Pubmed ID: 30786229 PROTEINS, INTERFACES, AND CRYO-EM GRIDS Current Opinion in Colloid & Interface Science. Mar, 2018 | Pubmed ID: 29867291 Estimating the Effect of Finite Depth of Field in Single-particle Cryo-EM Ultramicroscopy. Jan, 2018 | Pubmed ID: 28869854 How Cryo-EM Became So Hot Cell. Nov, 2017 | Pubmed ID: 29195065 Opinion: Hazards Faced by Macromolecules when Confined to Thin Aqueous Films Biophysics Reports. 2017 | Pubmed ID: 28781996 Multi-pass Transmission Electron Microscopy Scientific Reports. May, 2017 | Pubmed ID: 28490730 Monolayer-crystal Streptavidin Support Films Provide an Internal Standard of Cryo-EM Image Quality Journal of Structural Biology. Dec, 2017 | Pubmed ID: 28259651 Specimen Behavior in the Electron Beam Methods in Enzymology. 2016 | Pubmed ID: 27572722 Long Shelf-life Streptavidin Support-films Suitable for Electron Microscopy of Biological Macromolecules Journal of Structural Biology. Aug, 2016 | Pubmed ID: 27320699 Protein Complexes in Focus ELife. Apr, 2016 | Pubmed ID: 27124202 How Good Can Cryo-EM Become? Nature Methods. Jan, 2016 | Pubmed ID: 26716559 Factors That Influence the Formation and Stability of Thin, Cryo-EM Specimens Biophysical Journal. Feb, 2016 | Pubmed ID: 26386606 Automated Particle Correspondence and Accurate Tilt-axis Detection in Tilted-image Pairs Journal of Structural Biology. Jul, 2014 | Pubmed ID: 24694675 Disulfide Linkage and Structure of Highly Stable Yeast-derived Virus-like Particles of Murine Polyomavirus The Journal of Biological Chemistry. Apr, 2014 | Pubmed ID: 24567335 Generalization of the Matsumoto-Tonomura Approximation for the Phase Shift Within an Open Aperture Ultramicroscopy. Mar, 2014 | Pubmed ID: 24333773 Invited Review Article: Methods for Imaging Weak-phase Objects in Electron Microscopy The Review of Scientific Instruments. Nov, 2013 | Pubmed ID: 24289381 Replication and Validation of Cryo-EM Structures Journal of Structural Biology. Nov, 2013 | Pubmed ID: 24036314 Optimal and Fast Rotational Alignment of Volumes with Missing Data in Fourier Space Journal of Structural Biology. Nov, 2013 | Pubmed ID: 23994045 Minimizing Electrostatic Charging of an Aperture Used to Produce In-focus Phase Contrast in the TEM Ultramicroscopy. Dec, 2013 | Pubmed ID: 23872037 Ranking TEM Cameras by Their Response to Electron Shot Noise Ultramicroscopy. Oct, 2013 | Pubmed ID: 23747527 Deprotonation of D96 in Bacteriorhodopsin Opens the Proton Uptake Pathway Structure (London, England : 1993). Feb, 2013 | Pubmed ID: 23394942 Electron Microscopy of Biological Specimens in Liquid Water Biophysical Journal. Jul, 2012 | Pubmed ID: 22828343 Electron Microscopy of Biotinylated Protein Complexes Bound to Streptavidin Monolayer Crystals Journal of Structural Biology. Oct, 2012 | Pubmed ID: 22584152 Human Tripeptidyl Peptidase II: a Gentle Giant Structure (London, England : 1993). Apr, 2012 | Pubmed ID: 22483101 Design of a Hybrid Double-sideband/single-sideband (schlieren) Objective Aperture Suitable for Electron Microscopy Ultramicroscopy. Dec, 2011 | Pubmed ID: 22088443 Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects Biophysical Journal. May, 2011 | Pubmed ID: 21575566 The Surface of Evaporated Carbon Films is an Insulating, High-bandgap Material Journal of Structural Biology. May, 2011 | Pubmed ID: 21338687 Precise Beam-tilt Alignment and Collimation Are Required to Minimize the Phase Error Associated with Coma in High-resolution Cryo-EM Journal of Structural Biology. Apr, 2011 | Pubmed ID: 21182964 Umfrage Der Große Proteinkomplexe in D. Vulgaris Zeigt Großen Strukturellen Vielfalt Proceedings of the National Academy of Sciences of the United States of America. Sep, 2009 | Pubmed ID: 19805340 A Method for the Alignment of Heterogeneous Macromolecules from Electron Microscopy Journal of Structural Biology. Apr, 2009 | Pubmed ID: 19166941 Practical Factors Affecting the Performance of a Thin-film Phase Plate for Transmission Electron Microscopy Ultramicroscopy. Mar, 2009 | Pubmed ID: 19157711 Retrospective on the Early Development of Cryoelectron Microscopy of Macromolecules and a Prospective on Opportunities for the Future Journal of Structural Biology. Sep, 2008 | Pubmed ID: 18606231 Retrospective: Radiation Damage and Its Associated "information Limitations" Journal of Structural Biology. Sep, 2008 | Pubmed ID: 18588985 Restoration of Weak Phase-contrast Images Recorded with a High Degree of Defocus: the "twin Image" Problem Associated with CTF Correction Ultramicroscopy. Aug, 2008 | Pubmed ID: 18508199 Macromolecular Structures Without Crystals Proceedings of the National Academy of Sciences of the United States of America. Feb, 2008 | Pubmed ID: 18256181 Octomeric Pyruvat-Ferredoxin-Oxidoreduktase Von Desulfovibrio Vulgaris Journal of Structural Biology. Jul, 2007 | Pubmed ID: 17400475 Design of a Microfabricated, Two-electrode Phase-contrast Element Suitable for Electron Microscopy Ultramicroscopy. 2007 | Pubmed ID: 17079082 SPARX, Eine Neue Umgebung Für Cryo-EM-Bildverarbeitung Journal of Structural Biology. Jan, 2007 | Pubmed ID: 16931051 Stroboscopic Image Capture: Reducing the Dose Per Frame by a Factor of 30 Does Not Prevent Beam-induced Specimen Movement in Paraffin Ultramicroscopy. 2007 | Pubmed ID: 16905258 The Parallelization of SPIDER on Distributed-memory Computers Using MPI Journal of Structural Biology. Jan, 2007 | Pubmed ID: 16859923 Particle Picking by Segmentation: a Comparative Study with SPIDER-based Manual Particle Picking Journal of Structural Biology. Dec, 2005 | Pubmed ID: 16330229 High-Throughput-Film-Densitometrie: Ein Effizienter Ansatz, Um Große Datenmengen Zu Generieren Journal of Structural Biology. Jan, 2005 | Pubmed ID: 15629654 Experimental Characterization and Mitigation of Specimen Charging on Thin Films with One Conducting Layer Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. Dec, 2004 | Pubmed ID: 19780320 Electron Microscopy of Biological Macromolecules: Bridging the Gap Between What Physics Allows and What We Currently Can Get Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. Feb, 2004 | Pubmed ID: 15306063 Specificity of Anion Binding in the Substrate Pocket of Bacteriorhodopsin Biochemistry. May, 2004 | Pubmed ID: 15109251 Crystal Structure of the Bromide-bound D85S Mutant of Bacteriorhodopsin: Principles of Ion Pumping Biophysical Journal. Jul, 2003 | Pubmed ID: 12829500 Crystallization of Membrane Proteins from Media Composed of Connected-bilayer Gels Biopolymers. 2002 | Pubmed ID: 12539259 A Giant Protease with a Twist: the TPP II Complex from Drosophila Studied by Electron Microscopy The EMBO Journal. Nov, 2002 | Pubmed ID: 12426370 Modeling Chemical Bonding Effects for Protein Electron Crystallography: the Transferable Fragmental Electrostatic Potential (TFESP) Method Acta Crystallographica. Section A, Foundations of Crystallography. Mar, 2002 | Pubmed ID: 11832586 Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach Trinity Cookis1, Paul Sauer2,3, Simon Poepsel4,5, Bong-Gyoon Han6, Dominik A. Herbst1,2,6, Robert Glaeser6, Eva Nogales1,2,3,6 1Department of Molecular and Cell Biology, University of California, Berkeley, 2California Institute for Quantitative Biology (QB3), University of California, Berkeley, 3Howard Hughes Medical Institute, University of California, Berkeley, 4Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, 5Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, 6Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory JoVE 66197 Biochemistry
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach Trinity Cookis1, Paul Sauer2,3, Simon Poepsel4,5, Bong-Gyoon Han6, Dominik A. Herbst1,2,6, Robert Glaeser6, Eva Nogales1,2,3,6 1Department of Molecular and Cell Biology, University of California, Berkeley, 2California Institute for Quantitative Biology (QB3), University of California, Berkeley, 3Howard Hughes Medical Institute, University of California, Berkeley, 4Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, 5Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, 6Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory JoVE 66197 Biochemistry