William M. Old Department of Molecular, Cellular and Developmental Biology University of Colorado Biography Publications Institution JoVE Articles William M. Old has not added a biography. If you are William M. Old and would like to personalize this page please email our Author Liaison for assistance. Publications Hidden Resources in the Genome Restore PLP Synthesis and Robust Growth After Deletion of the Essential Gene Proceedings of the National Academy of Sciences of the United States of America. Nov, 2019 | Pubmed ID: 31712440 Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically Encoded Biosensors Methods in Molecular Biology (Clifton, N.J.). Month, 2019 | Pubmed ID: 31119673 The Nuclear Interactome of DYRK1A Reveals a Functional Role in DNA Damage Repair Scientific Reports. Apr, 2019 | Pubmed ID: 31024071 A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission Cell. 09, 2018 | Pubmed ID: 30220460 Synonymous Mutations Make Dramatic Contributions to Fitness when Growth is Limited by a Weak-link Enzyme PLoS Genetics. 08, 2018 | Pubmed ID: 30148850 Dengue Viruses Cleave STING in Humans but Not in Nonhuman Primates, Their Presumed Natural Reservoir ELife. 03, 2018 | Pubmed ID: 29557779 Specificity of Phosphorylation Responses to Mitogen Activated Protein (MAP) Kinase Pathway Inhibitors in Melanoma Cells Molecular & Cellular Proteomics : MCP. 04, 2018 | Pubmed ID: 29255136 Identification of the Primary Peptide Contaminant That Inhibits Fibrillation and Toxicity in Synthetic Amyloid-β42 PloS One. Month, 2017 | Pubmed ID: 28792968 Human TFIIH Kinase CDK7 Regulates Transcription-Associated Chromatin Modifications Cell Reports. 08, 2017 | Pubmed ID: 28768201 A Synonymous Mutation Upstream of the Gene Encoding a Weak-Link Enzyme Causes an Ultrasensitive Response in Growth Rate Journal of Bacteriology. 10, 2016 | Pubmed ID: 27501982 Identification of Mediator Kinase Substrates in Human Cells Using Cortistatin A and Quantitative Phosphoproteomics Cell Reports. Apr, 2016 | Pubmed ID: 27050516 Erratum To: Large-Scale Examination of Factors Influencing Phosphopeptide Neutral Loss During Collision Induced Dissociation Journal of the American Society for Mass Spectrometry. Oct, 2015 | Pubmed ID: 26169511 Centromere Protein F Includes Two Sites That Couple Efficiently to Depolymerizing Microtubules The Journal of Cell Biology. Jun, 2015 | Pubmed ID: 26101217 Large-Scale Examination of Factors Influencing Phosphopeptide Neutral Loss During Collision Induced Dissociation Journal of the American Society for Mass Spectrometry. Jul, 2015 | Pubmed ID: 25851653 A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells Molecular & Cellular Proteomics : MCP. Jun, 2015 | Pubmed ID: 25850435 Identification of a Family of Fatty-acid-speciated Sonic Hedgehog Proteins, Whose Members Display Differential Biological Properties Cell Reports. Mar, 2015 | Pubmed ID: 25732819 Gas-phase Acidities of Nitrated Azoles As Determined by the Extended Kinetic Method and Computations The Journal of Physical Chemistry. A. Jan, 2015 | Pubmed ID: 25521708 Development of Substrate-selective Probes for Affinity Pulldown of Histone Demethylases ACS Chemical Biology. Jan, 2015 | Pubmed ID: 25335116 Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 PLoS Genetics. Oct, 2014 | Pubmed ID: 25340401 Identification of Global Ferredoxin Interaction Networks in Chlamydomonas Reinhardtii The Journal of Biological Chemistry. Dec, 2013 | Pubmed ID: 24100040 The Non-coding B2 RNA Binds to the DNA Cleft and Active-site Region of RNA Polymerase II Journal of Molecular Biology. Oct, 2013 | Pubmed ID: 23416138 IsoformResolver: A Peptide-centric Algorithm for Protein Inference Journal of Proteome Research. Jul, 2011 | Pubmed ID: 21599010 Spectrum-to-spectrum Searching Using a Proteome-wide Spectral Library Molecular & Cellular Proteomics : MCP. Jul, 2011 | Pubmed ID: 21532008 Quantifying the Impact of Chimera MS/MS Spectra on Peptide Identification in Large-scale Proteomics Studies Journal of Proteome Research. Aug, 2010 | Pubmed ID: 20578722 Functional Proteomics Identifies Targets of Phosphorylation by B-Raf Signaling in Melanoma Molecular Cell. Apr, 2009 | Pubmed ID: 19362540 Budding Yeast Centrosome Duplication Requires Stabilization of Spc29 Via Mps1-mediated Phosphorylation The Journal of Biological Chemistry. May, 2009 | Pubmed ID: 19269975 A Simulated MS/MS Library for Spectrum-to-spectrum Searching in Large Scale Identification of Proteins Molecular & Cellular Proteomics : MCP. Apr, 2009 | Pubmed ID: 19106086 Mapping Protein Post-translational Modifications with Mass Spectrometry Nature Methods. Oct, 2007 | Pubmed ID: 17901869 Mps1 Activation Loop Autophosphorylation Enhances Kinase Activity The Journal of Biological Chemistry. Oct, 2007 | Pubmed ID: 17728254 Achieving In-depth Proteomics Profiling by Mass Spectrometry ACS Chemical Biology. Jan, 2007 | Pubmed ID: 17243782 Improved Validation of Peptide MS/MS Assignments Using Spectral Intensity Prediction Molecular & Cellular Proteomics : MCP. Jan, 2007 | Pubmed ID: 17018520 Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics Molecular & Cellular Proteomics : MCP. Oct, 2005 | Pubmed ID: 15979981 Improving Reproducibility and Sensitivity in Identifying Human Proteins by Shotgun Proteomics Analytical Chemistry. Jul, 2004 | Pubmed ID: 15228325 Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling Steven E. Guard1, Christopher C. Ebmeier1, William M. Old1,2 1Department of Molecular, Cellular and Developmental Biology, University of Colorado, 2Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine JoVE 60432 Biochemistry
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling Steven E. Guard1, Christopher C. Ebmeier1, William M. Old1,2 1Department of Molecular, Cellular and Developmental Biology, University of Colorado, 2Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine JoVE 60432 Biochemistry