William Kwok Kwok Laboratory Benaroya Research Institute Biography Publications Institution JoVE Articles William Kwok has not added a biography. If you are William Kwok and would like to personalize this page please email our Author Liaison for assistance. Publications Frequency of Epitope-Specific Naive CD4+ T Cells Correlates with Immunodominance in the Human Memory Repertoire Journal of Immunology (Baltimore, Md. : 1950). Feb, 2012 | Pubmed ID: 22327072 Broadly Directed Virus-specific CD4+ T Cell Responses Are Primed During Acute Hepatitis C Infection, but Rapidly Disappear from Human Blood with Viral Persistence The Journal of Experimental Medicine. Jan, 2012 | Pubmed ID: 22213804 DRB1*12:01 Presents a Unique Subset of Epitopes by Preferring Aromatics in Pocket 9 Molecular Immunology. Feb, 2012 | Pubmed ID: 22196942 Induction and Maintenance of Allergen-specific FOXP3(+) Treg Cells in Human Tonsils As Potential First-line Organs of Oral Tolerance The Journal of Allergy and Clinical Immunology. Feb, 2012 | Pubmed ID: 22051696 Differentiation Stage Determines Pathologic and Protective Allergen-specific CD4(+) T-cell Outcomes During Specific Immunotherapy The Journal of Allergy and Clinical Immunology. Feb, 2012 | Pubmed ID: 21975176 Acquisition of Pneumococci Specific Effector and Regulatory Cd4+ T Cells Localising Within Human Upper Respiratory-tract Mucosal Lymphoid Tissue PLoS Pathogens. Dec, 2011 | Pubmed ID: 22144893 Human CD8⺠and CD4⺠T Cell Memory to Lymphocytic Choriomeningitis Virus Infection Journal of Virology. Nov, 2011 | Pubmed ID: 21900169 Uveitis-associated Epitopes of Retinal Antigens Are Pathogenic in the Humanized Mouse Model of Uveitis and Identify Autoaggressive T Cells Journal of Immunology (Baltimore, Md. : 1950). Aug, 2011 | Pubmed ID: 21765017 Increased Frequencies of Myelin Oligodendrocyte Glycoprotein/MHC Class II-binding CD4 Cells in Patients with Multiple Sclerosis Journal of Immunology (Baltimore, Md. : 1950). Jul, 2011 | Pubmed ID: 21653833 Autoreactive CD4(+) T Cells in Patients with Atopic Dermatitis The Journal of Allergy and Clinical Immunology. Jul, 2011 | Pubmed ID: 21620451 Papillomavirus-specific CD4+ T Cells Exhibit Reduced STAT-5 Signaling and Altered Cytokine Profiles in Patients with Recurrent Respiratory Papillomatosis Journal of Immunology (Baltimore, Md. : 1950). Jun, 2011 | Pubmed ID: 21531896 Ara H 1-reactive T Cells in Individuals with Peanut Allergy The Journal of Allergy and Clinical Immunology. May, 2011 | Pubmed ID: 21459424 Autoantigen-specific Regulatory T Cells, a Potential Tool for Immune-tolerance Reconstitution in Type-2 Autoimmune Hepatitis Hepatology (Baltimore, Md.). Feb, 2011 | Pubmed ID: 21274874 Differences in Self-peptide Binding Between T1D-related Susceptible and Protective DR4 Subtypes Journal of Autoimmunity. Mar, 2011 | Pubmed ID: 21257290 Autoantigen-specific Regulatory T Cells, a Potential Tool for Immune-tolerance Reconstitution in Type-2 Autoimmune Hepatitis Hepatology (Baltimore, Md.). Oct, 2010 | Pubmed ID: 21190158 Allergen-specific Naïve and Memory CD4+ T Cells Exhibit Functional and Phenotypic Differences Between Individuals with or Without Allergy European Journal of Immunology. Sep, 2010 | Pubmed ID: 20690179 Peptide-MHC Cellular Microarray with Innovative Data Analysis System for Simultaneously Detecting Multiple CD4 T-cell Responses PloS One. 2010 | Pubmed ID: 20634998 HLA-DR1001 Presents "altered-self" Peptides Derived from Joint-associated Proteins by Accepting Citrulline in Three of Its Binding Pockets Arthritis and Rheumatism. Oct, 2010 | Pubmed ID: 20533291 Direct Ex Vivo Analysis of Allergen-specific CD4+ T Cells The Journal of Allergy and Clinical Immunology. Jun, 2010 | Pubmed ID: 20513526 HIV Controller CD4+ T Cells Respond to Minimal Amounts of Gag Antigen Due to High TCR Avidity PLoS Pathogens. Feb, 2010 | Pubmed ID: 20195518 Involvement of CD91 and Scavenger Receptors in Hsp70-facilitated Activation of Human Antigen-specific CD4+ Memory T Cells European Journal of Immunology. Apr, 2010 | Pubmed ID: 20101615 Assessment of Seasonal Influenza A Virus-specific CD4 T-cell Responses to 2009 Pandemic H1N1 Swine-origin Influenza A Virus Journal of Virology. Apr, 2010 | Pubmed ID: 20071564 Reassessing the Role of HLA-DRB3 T-cell Responses: Evidence for Significant Expression and Complementary Antigen Presentation European Journal of Immunology. Jan, 2010 | Pubmed ID: 19830726 Tumor Antigen-specific FOXP3+ CD4 T Cells Identified in Human Metastatic Melanoma: Peptide Vaccination Results in Selective Expansion of Th1-like Counterparts Cancer Research. Oct, 2009 | Pubmed ID: 19808957 The Binding of Antigenic Peptides to HLA-DR is Influenced by Interactions Between Pocket 6 and Pocket 9 Journal of Immunology (Baltimore, Md. : 1950). Sep, 2009 | Pubmed ID: 19648278 Lineages of Human T-cell Clones, Including T Helper 17/T Helper 1 Cells, Isolated at Different Stages of Anti-factor VIII Immune Responses Blood. Aug, 2009 | Pubmed ID: 19549986 H5N1 Strain-specific Hemagglutinin CD4+ T Cell Epitopes Restricted by HLA DR4 Vaccine. Jun, 2009 | Pubmed ID: 19446935 Functional Islet-specific Treg Can Be Generated from CD4+CD25- T Cells of Healthy and Type 1 Diabetic Subjects European Journal of Immunology. Feb, 2009 | Pubmed ID: 19180473 Searching Immunodominant Epitopes Prior to Epidemic: HLA Class II-restricted SARS-CoV Spike Protein Epitopes in Unexposed Individuals International Immunology. Jan, 2009 | Pubmed ID: 19050106 The Anthrax Vaccine Adsorbed Vaccine Generates Protective Antigen (PA)-Specific CD4+ T Cells with a Phenotype Distinct from That of Naive PA T Cells Infection and Immunity. Oct, 2008 | Pubmed ID: 18678674 Low-affinity Major Histocompatibility Complex-binding Peptides in Type 1 Diabetes Diabetes. Jul, 2008 | Pubmed ID: 18586912 CD4+ T Cells from Type 1 Diabetic and Healthy Subjects Exhibit Different Thresholds of Activation to a Naturally Processed Proinsulin Epitope Journal of Autoimmunity. Aug, 2008 | Pubmed ID: 18385016 Identification and in Vitro Expansion of Functional Antigen-specific CD25+ FoxP3+ Regulatory T Cells in Hepatitis C Virus Infection Journal of Virology. May, 2008 | Pubmed ID: 18337568 Definition of the Peptide Binding Motif Within DRB1*1401 Restricted Epitopes by Peptide Competition and Structural Modeling Molecular Immunology. May, 2008 | Pubmed ID: 18276010 Healthy Human Subjects Have CD4+ T Cells Directed Against H5N1 Influenza Virus Journal of Immunology (Baltimore, Md. : 1950). Feb, 2008 | Pubmed ID: 18209073 Searching for Borrelial T Cell Epitopes Associated with Antibiotic-refractory Lyme Arthritis Molecular Immunology. Apr, 2008 | Pubmed ID: 18191206 High Level of PD-1 Expression on Hepatitis C Virus (HCV)-specific CD8+ and CD4+ T Cells During Acute HCV Infection, Irrespective of Clinical Outcome Journal of Virology. Mar, 2008 | Pubmed ID: 18160439 Human Homologues of a Borrelia T Cell Epitope Associated with Antibiotic-refractory Lyme Arthritis Molecular Immunology. Jan, 2008 | Pubmed ID: 17555819 Tracking Antigen Specific CD4+ T-cells with Soluble MHC Molecules Methods in Molecular Medicine. 2007 | Pubmed ID: 17983139 Decline in the Frequencies of Borrelia Burgdorferi OspA161 175-specific T Cells After Antibiotic Therapy in HLA-DRB1*0401-positive Patients with Antibiotic-responsive or Antibiotic-refractory Lyme Arthritis Journal of Immunology (Baltimore, Md. : 1950). Nov, 2007 | Pubmed ID: 17947711 Tetramer-guided Epitope Mapping Reveals Broad, Individualized Repertoires of Tetanus Toxin-specific CD4+ T Cells and Suggests HLA-based Differences in Epitope Recognition International Immunology. Nov, 2007 | Pubmed ID: 17906339 Dominance and Diversity in the Primary Human CD4 T Cell Response to Replication-competent Vaccinia Virus Journal of Immunology (Baltimore, Md. : 1950). May, 2007 | Pubmed ID: 17475867 Potential of an Altered Peptide Ligand of Lipocalin Allergen Bos D 2 for Peptide Immunotherapy The Journal of Allergy and Clinical Immunology. Apr, 2007 | Pubmed ID: 17335888 Antigen-specific CD4+ T Cells Recognize Epitopes of Protective Antigen Following Vaccination with an Anthrax Vaccine Infection and Immunity. Apr, 2007 | Pubmed ID: 17283103 CD8+ Suppressor-mediated Regulation of Human CD4+ T Cell Responses to Glutamic Acid Decarboxylase 65 European Journal of Immunology. Jan, 2007 | Pubmed ID: 17163450 Detection of Low Avidity Desmoglein 3-reactive T Cells in Pemphigus Vulgaris Using HLA-DR Beta 1*0402 Tetramers Clinical Immunology (Orlando, Fla.). Mar, 2007 | Pubmed ID: 17113829 Melan-A/MART-1-specific CD4 T Cells in Melanoma Patients: Identification of New Epitopes and Ex Vivo Visualization of Specific T Cells by MHC Class II Tetramers Journal of Immunology (Baltimore, Md. : 1950). Nov, 2006 | Pubmed ID: 17082590 HLA-DQ2 and -DQ8 Signatures of Gluten T Cell Epitopes in Celiac Disease The Journal of Clinical Investigation. Aug, 2006 | Pubmed ID: 16878175 Multiplex Mapping of CD4 T Cell Epitopes Using Class II Tetramers Clinical Immunology (Orlando, Fla.). Jul, 2006 | Pubmed ID: 16677863 Allergen-specific MHC Class II Tetramer+ Cells Are Detectable in Allergic, but Not in Nonallergic, Individuals Journal of Immunology (Baltimore, Md. : 1950). Apr, 2006 | Pubmed ID: 16585604 Antibiotic-refractory Lyme Arthritis is Associated with HLA-DR Molecules That Bind a Borrelia Burgdorferi Peptide The Journal of Experimental Medicine. Apr, 2006 | Pubmed ID: 16585267 Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein-reactive CD4+ T Cells in Human Subjects Journal of Immunology (Baltimore, Md. : 1950). Mar, 2006 | Pubmed ID: 16493034 Cytotoxic Herpes Simplex Type 2-specific, DQ0602-restricted CD4 T+-cell Clones Show Alloreactivity to DQ0601 Immunology. Mar, 2006 | Pubmed ID: 16476054 Allelic Variation in Key Peptide-binding Pockets Discriminates Between Closely Related Diabetes-protective and Diabetes-susceptible HLA-DQB1*06 Alleles Journal of Immunology (Baltimore, Md. : 1950). Feb, 2006 | Pubmed ID: 16424231 GAD65- and Proinsulin-specific CD4+ T-cells Detected by MHC Class II Tetramers in Peripheral Blood of Type 1 Diabetes Patients and At-risk Subjects Journal of Autoimmunity. Nov, 2005 | Pubmed ID: 16263242 Comparative Study of GAD65-specific CD4+ T Cells in Healthy and Type 1 Diabetic Subjects Journal of Autoimmunity. Dec, 2005 | Pubmed ID: 16249070 The Heat Shock Protein Hsp70 Enhances Antigen-specific Proliferation of Human CD4+ Memory T Cells European Journal of Immunology. Nov, 2005 | Pubmed ID: 16245362 Immunomodulatory Potential of Heteroclitic Analogs of the Dominant T-cell Epitope of Lipocalin Allergen Bos D 2 on Specific T Cells International Immunology. Dec, 2005 | Pubmed ID: 16221722 Expression of HLA-DP0401 Molecules for Identification of DP0401 Restricted Antigen Specific T Cells Journal of Clinical Immunology. Sep, 2005 | Pubmed ID: 16160911 Persistence of Herpes Simplex Virus Type 2 VP16-specific CD4+ T Cells Human Immunology. Jul, 2005 | Pubmed ID: 16112025 Functional Avidity Directs T-cell Fate in Autoreactive CD4+ T Cells Blood. Oct, 2005 | Pubmed ID: 16030184 Clustering of T Cell Ligands on Artificial APC Membranes Influences T Cell Activation and Protein Kinase C Theta Translocation to the T Cell Plasma Membrane Journal of Immunology (Baltimore, Md. : 1950). Mar, 2005 | Pubmed ID: 15749850 Expression of Cutaneous Lymphocyte-associated Antigen and E-selectin Ligand by Circulating Human Memory CD4+ T Lymphocytes Specific for Herpes Simplex Virus Type 2 The Journal of Infectious Diseases. Jan, 2005 | Pubmed ID: 15609235 In Vivo Biotinylation of the Major Histocompatibility Complex (MHC) Class II/peptide Complex by Coexpression of BirA Enzyme for the Generation of MHC Class II/tetramers Human Immunology. Jul, 2004 | Pubmed ID: 15301857 GAD65-specific CD4+ T-cells with High Antigen Avidity Are Prevalent in Peripheral Blood of Patients with Type 1 Diabetes Diabetes. Aug, 2004 | Pubmed ID: 15277377 Extensive Replicative Capacity of Human Central Memory T Cells Journal of Immunology (Baltimore, Md. : 1950). Jun, 2004 | Pubmed ID: 15153483 Autoreactive T Cells in Healthy Individuals Journal of Immunology (Baltimore, Md. : 1950). May, 2004 | Pubmed ID: 15128778 Differential Recognition and Activation Thresholds in Human Autoreactive GAD-specific T-cells Diabetes. Apr, 2004 | Pubmed ID: 15047612 HLA Class II Polymorphisms Determine Responses to Bacterial Superantigens Journal of Immunology (Baltimore, Md. : 1950). Feb, 2004 | Pubmed ID: 14734754 Direct Killing of Epstein-Barr Virus (EBV)-infected B Cells by CD4 T Cells Directed Against the EBV Lytic Protein BHRF1 Blood. Feb, 2004 | Pubmed ID: 14563644 Detection of CD4+ Autoreactive T Cells in T1D Using HLA Class II Tetramers Annals of the New York Academy of Sciences. Nov, 2003 | Pubmed ID: 14679042 Diminished Proliferation of Human Immunodeficiency Virus-specific CD4+ T Cells is Associated with Diminished Interleukin-2 (IL-2) Production and is Recovered by Exogenous IL-2 Journal of Virology. Oct, 2003 | Pubmed ID: 14512540 HLA Class II-restricted CD4+ T Cell Responses Directed Against Influenza Viral Antigens Postinfluenza Vaccination Journal of Immunology (Baltimore, Md. : 1950). Sep, 2003 | Pubmed ID: 12960344 P53(110-124)-specific Human CD4+ T-helper Cells Enhance in Vitro Generation and Antitumor Function of Tumor-reactive CD8+ T Cells Cancer Research. Jul, 2003 | Pubmed ID: 12839958 Low-avidity Recognition by CD4+ T Cells Directed to Self-antigens European Journal of Immunology. May, 2003 | Pubmed ID: 12731068 Use of HLA Class II Tetramers in Tracking Antigen-specific T Cells and Mapping T-cell Epitopes Methods (San Diego, Calif.). Mar, 2003 | Pubmed ID: 12725793 Challenges in Staining T Cells Using HLA Class II Tetramers Clinical Immunology (Orlando, Fla.). Jan, 2003 | Pubmed ID: 12584047 HLA Tetramer-based Artificial Antigen-presenting Cells for Stimulation of CD4+ T Cells Clinical Immunology (Orlando, Fla.). Jan, 2003 | Pubmed ID: 12584046 Differential Antigen Sensitivity and Costimulatory Requirements in Human Th1 and Th2 Antigen-specific CD4+ Cells with Similar TCR Avidity Journal of Immunology (Baltimore, Md. : 1950). Feb, 2003 | Pubmed ID: 12538679 Use of Class II Tetramers for Identification of CD4+ T Cells Journal of Immunological Methods. Oct, 2002 | Pubmed ID: 12213344 Disease-associated Bias in T Helper Type 1 (Th1)/Th2 CD4(+) T Cell Responses Against MAGE-6 in HLA-DRB10401(+) Patients with Renal Cell Carcinoma or Melanoma The Journal of Experimental Medicine. Sep, 2002 | Pubmed ID: 12208877 Defining Antigen-specific Responses with Human MHC Class II Tetramers The Journal of Allergy and Clinical Immunology. Aug, 2002 | Pubmed ID: 12170258 HLA Class II Peptide-binding and Autoimmunity Tissue Antigens. Feb, 2002 | Pubmed ID: 12028533 Linear B-cell Epitopes in Lambert-Eaton Myasthenic Syndrome Defined by Cell-free Synthetic Peptide Binding Journal of Neuroimmunology. May, 2002 | Pubmed ID: 12020970 Detection of GAD65-specific T-cells by Major Histocompatibility Complex Class II Tetramers in Type 1 Diabetic Patients and At-risk Subjects Diabetes. May, 2002 | Pubmed ID: 11978633 Mutational Analysis of Critical Residues Determining Antigen Presentation and Activation of HLA-DQ0602 Restricted T-cell Clones Human Immunology. Mar, 2002 | Pubmed ID: 11872236 HLA Class II Tetramers: Tools for Direct Analysis of Antigen-specific CD4+ T Cells Arthritis and Rheumatism. Jan, 2002 | Pubmed ID: 11817608 Identification of Type II Collagen Peptide 261-273-specific T Cell Clones in a Patient with Relapsing Polychondritis Arthritis and Rheumatism. Jan, 2002 | Pubmed ID: 11817597 Antigen CD4 + הדמיה ספציפיות תאים T באמצעות MHC Class II Tetramers Eddie A. James1, Rebecca LaFond2, Ivana Durinovic-Bello3, William Kwok2 1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute JoVE 1167 Biology
Antigen CD4 + הדמיה ספציפיות תאים T באמצעות MHC Class II Tetramers Eddie A. James1, Rebecca LaFond2, Ivana Durinovic-Bello3, William Kwok2 1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute JoVE 1167 Biology