Wolfgang Wurst Institute of Developmental Genetics, Helmholtz Zentrum German Research Center for Environmental Health Biography Publications Institution JoVE Articles Wolfgang Wurst has not added a biography. If you are Wolfgang Wurst and would like to personalize this page please email our Author Liaison for assistance. Publications A Mouse Model for Intellectual Disability Caused by Mutations in the X-linked 2'‑O‑methyltransferase Ftsj1 Gene Biochimica Et Biophysica Acta. Molecular Basis of Disease. Dec, 2018 | Pubmed ID: 30557699 The Trem2 R47H Alzheimer's Risk Variant Impairs Splicing and Reduces Trem2 MRNA and Protein in Mice but Not in Humans Molecular Neurodegeneration. 09, 2018 | Pubmed ID: 30185230 Exosomal Secretion of α-synuclein As Protective Mechanism After Upstream Blockage of Macroautophagy Cell Death & Disease. Jul, 2018 | Pubmed ID: 29988147 Laboratory Mouse Housing Conditions Can Be Improved Using Common Environmental Enrichment Without Compromising Data PLoS Biology. Apr, 2018 | Pubmed ID: 29659570 Identification of Genetic Elements in Metabolism by High-throughput Mouse Phenotyping Nature Communications. 01, 2018 | Pubmed ID: 29348434 Lifetime Study in Mice After Acute Low-dose Ionizing Radiation: a Multifactorial Study with Special Focus on Cataract Risk Radiation and Environmental Biophysics. 05, 2018 | Pubmed ID: 29327260 Understanding Gene Functions and Disease Mechanisms: Phenotyping Pipelines in the German Mouse Clinic Behavioural Brain Research. 10, 2018 | Pubmed ID: 28966146 Fgf9 Mutation Alters Information Processing and Social Memory in Mice Molecular Neurobiology. Jun, 2018 | Pubmed ID: 28695538 Heterozygosity for the Mood Disorder-Associated Variant Gln460Arg Alters P2X7 Receptor Function and Sleep Quality The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 2017 | Pubmed ID: 29079688 A Large Scale Hearing Loss Screen Reveals an Extensive Unexplored Genetic Landscape for Auditory Dysfunction Nature Communications. 10, 2017 | Pubmed ID: 29026089 Every-other-day Feeding Extends Lifespan but Fails to Delay Many Symptoms of Aging in Mice Nature Communications. Jul, 2017 | Pubmed ID: 28761067 Disease Model Discovery from 3,328 Gene Knockouts by The International Mouse Phenotyping Consortium Nature Genetics. Aug, 2017 | Pubmed ID: 28650483 The Pathogenic LRRK2 R1441C Mutation Induces Specific Deficits Modeling the Prodromal Phase of Parkinson's Disease in the Mouse Neurobiology of Disease. Sep, 2017 | Pubmed ID: 28576705 The FTD-like Syndrome Causing TREM2 T66M Mutation Impairs Microglia Function, Brain Perfusion, and Glucose Metabolism The EMBO Journal. 07, 2017 | Pubmed ID: 28559417 Spinal Poly-GA Inclusions in a C9orf72 Mouse Model Trigger Motor Deficits and Inflammation Without Neuron Loss Acta Neuropathologica. 08, 2017 | Pubmed ID: 28409281 Control of Gene Editing by Manipulation of DNA Repair Mechanisms Mammalian Genome : Official Journal of the International Mammalian Genome Society. Aug, 2017 | Pubmed ID: 28374058 Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System Methods (San Diego, Calif.). 05, 2017 | Pubmed ID: 28263886 Role of Mitochondrial Metabolism in the Control of Early Lineage Progression and Aging Phenotypes in Adult Hippocampal Neurogenesis Neuron. Feb, 2017 | Pubmed ID: 28111078 Interplay Between H1 and HMGN Epigenetically Regulates OLIG1&2 Expression and Oligodendrocyte Differentiation Nucleic Acids Research. Apr, 2017 | Pubmed ID: 27923998 Genetically Dissecting P2rx7 Expression Within the Central Nervous System Using Conditional Humanized Mice Purinergic Signalling. 06, 2017 | Pubmed ID: 27858314 The First Scube3 Mutant Mouse Line with Pleiotropic Phenotypic Alterations G3 (Bethesda, Md.). Dec, 2016 | Pubmed ID: 27815347 Caspase-mediated Apoptosis Induction in Zebrafish Cerebellar Purkinje Neurons Development (Cambridge, England). 11, 2016 | Pubmed ID: 27729409 Viable Ednra (Y129F) Mice Feature Human Mandibulofacial Dysostosis with Alopecia (MFDA) Syndrome Due to the Homologue Mutation Mammalian Genome : Official Journal of the International Mammalian Genome Society. Dec, 2016 | Pubmed ID: 27671791 Animal Models Are Valid to Uncover Disease Mechanisms PLoS Genetics. 05, 2016 | Pubmed ID: 27227339 CRFR1 in AgRP Neurons Modulates Sympathetic Nervous System Activity to Adapt to Cold Stress and Fasting Cell Metabolism. Jun, 2016 | Pubmed ID: 27211900 Diversity Matters - Heterogeneity of Dopaminergic Neurons in the Ventral Mesencephalon and Its Relation to Parkinson's Disease Journal of Neurochemistry. 10, 2016 | Pubmed ID: 27206718 Diet-induced and Mono-genetic Obesity Alter Volatile Organic Compound Signature in Mice Journal of Breath Research. Feb, 2016 | Pubmed ID: 26860833 Differences in the Spatiotemporal Expression and Epistatic Gene Regulation of the Mesodiencephalic Dopaminergic Precursor Marker PITX3 During Chicken and Mouse Development Development (Cambridge, England). Feb, 2016 | Pubmed ID: 26755703 Genome Editing in Mice Using TALE Nucleases Methods in Molecular Biology (Clifton, N.J.). 2016 | Pubmed ID: 26443225 Assessing Cognition in Mice Current Protocols in Mouse Biology. Dec, 2015 | Pubmed ID: 26629775 Conditional Reduction of Adult Born Doublecortin-Positive Neurons Reversibly Impairs Selective Behaviors Frontiers in Behavioral Neuroscience. 2015 | Pubmed ID: 26617501 Dickkopf 3 Promotes the Differentiation of a Rostrolateral Midbrain Dopaminergic Neuronal Subset In Vivo and from Pluripotent Stem Cells In Vitro in the Mouse The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2015 | Pubmed ID: 26424886 Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated Via Its C-Terminal PDZ Binding Motif PloS One. 2015 | Pubmed ID: 26352593 Beyond Knockouts: the International Knockout Mouse Consortium Delivers Modular and Evolving Tools for Investigating Mammalian Genes Mammalian Genome : Official Journal of the International Mammalian Genome Society. Oct, 2015 | Pubmed ID: 26340938 Analysis of Mammalian Gene Function Through Broad-based Phenotypic Screens Across a Consortium of Mouse Clinics Nature Genetics. Jul, 2015 | Pubmed ID: 26214591 Development of an Intein-mediated Split-Cas9 System for Gene Therapy Nucleic Acids Research. Jul, 2015 | Pubmed ID: 26082496 MIM-Induced Membrane Bending Promotes Dendritic Spine Initiation Developmental Cell. Jun, 2015 | Pubmed ID: 26051541 A WNT1-regulated Developmental Gene Cascade Prevents Dopaminergic Neurodegeneration in Adult En1(+/-) Mice Neurobiology of Disease. Oct, 2015 | Pubmed ID: 26049140 Expression of a Catalytically Inactive Mutant Form of Glutathione Peroxidase 4 (Gpx4) Confers a Dominant-negative Effect in Male Fertility The Journal of Biological Chemistry. Jun, 2015 | Pubmed ID: 25922076 Increasing the Efficiency of Homology-directed Repair for CRISPR-Cas9-induced Precise Gene Editing in Mammalian Cells Nature Biotechnology. May, 2015 | Pubmed ID: 25803306 Creation of Targeted Genomic Deletions Using TALEN or CRISPR/Cas Nuclease Pairs in One-cell Mouse Embryos FEBS Open Bio. 2015 | Pubmed ID: 25685662 Mouse IDGenes: a Reference Database for Genetic Interactions in the Developing Mouse Brain Database : the Journal of Biological Databases and Curation. 2014 | Pubmed ID: 25145340 Pleiotropic Functions for Transcription Factor Zscan10 PloS One. 2014 | Pubmed ID: 25111779 Abnormal Brain Iron Metabolism in Irp2 Deficient Mice is Associated with Mild Neurological and Behavioral Impairments PloS One. 2014 | Pubmed ID: 24896637 High-throughput Phenotypic Assessment of Cardiac Physiology in Four Commonly Used Inbred Mouse Strains Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology. Aug, 2014 | Pubmed ID: 24788387 A Robust and Reliable Non-invasive Test for Stress Responsivity in Mice Frontiers in Behavioral Neuroscience. 2014 | Pubmed ID: 24782732 Restless Legs Syndrome-associated Intronic Common Variant in Meis1 Alters Enhancer Function in the Developing Telencephalon Genome Research. Apr, 2014 | Pubmed ID: 24642863 Generation of Targeted Mouse Mutants by Embryo Microinjection of TALENs Methods (San Diego, Calif.). Aug, 2014 | Pubmed ID: 24418396 Wnt1-regulated Genetic Networks in Midbrain Dopaminergic Neuron Development Journal of Molecular Cell Biology. Feb, 2014 | Pubmed ID: 24326514 Efficient Isolation of Pure and Functional Mitochondria from Mouse Tissues Using Automated Tissue Disruption and Enrichment with Anti-TOM22 Magnetic Beads PloS One. 2013 | Pubmed ID: 24349272 An RNAi-based Approach to Down-regulate a Gene Family in Vivo PloS One. 2013 | Pubmed ID: 24265806 Generation of Targeted Mouse Mutants by Embryo Microinjection of TALEN MRNA Nature Protocols. Dec, 2013 | Pubmed ID: 24177293 Highly Efficient Targeted Mutagenesis in Mice Using TALENs Genetics. Nov, 2013 | Pubmed ID: 23979585 Rapamycin Extends Murine Lifespan but Has Limited Effects on Aging The Journal of Clinical Investigation. Aug, 2013 | Pubmed ID: 23863708 Expression Analysis of Lrrk1, Lrrk2 and Lrrk2 Splice Variants in Mice PloS One. 2013 | Pubmed ID: 23675505 High Mobility Group N Proteins Modulate the Fidelity of the Cellular Transcriptional Profile in a Tissue- and Variant-specific Manner The Journal of Biological Chemistry. Jun, 2013 | Pubmed ID: 23620591 Direct Production of Mouse Disease Models by Embryo Microinjection of TALENs and Oligodeoxynucleotides Proceedings of the National Academy of Sciences of the United States of America. Mar, 2013 | Pubmed ID: 23426636 In Vitro Analysis of Bone Phenotypes in Col1a1 and Jagged1 Mutant Mice Using a Standardized Osteoblast Cell Culture System Journal of Bone and Mineral Metabolism. May, 2013 | Pubmed ID: 23371561 Innovaciones En Fenotipado De Modelos Murinos En La Clínica Alemana De Ratón Mammalian Genome : Official Journal of the International Mammalian Genome Society. Oct, 2012 | Pubmed ID: 22926221 Voluntary Wheel Running in Mice Increases the Rate of Neurogenesis Without Affecting Anxiety-related Behaviour in Single Tests BMC Neuroscience. Jun, 2012 | Pubmed ID: 22682077 Cardiopulmonary Dysfunction in the Osteogenesis Imperfecta Mouse Model Aga2 and Human Patients Are Caused by Bone-independent Mechanisms Human Molecular Genetics. Aug, 2012 | Pubmed ID: 22589248 MAPK Signaling Determines Anxiety in the Juvenile Mouse Brain but Depression-like Behavior in Adults PloS One. 2012 | Pubmed ID: 22529971 Neurobeachin, a Regulator of Synaptic Protein Targeting, is Associated with Body Fat Mass and Feeding Behavior in Mice and Body-mass Index in Humans PLoS Genetics. 2012 | Pubmed ID: 22438821 Pink1-deficiency in Mice Impairs Gait, Olfaction and Serotonergic Innervation of the Olfactory Bulb Experimental Neurology. May, 2012 | Pubmed ID: 22265660 Brain-specific Inactivation of the Crhr1 Gene Inhibits Post-dependent and Stress-induced Alcohol Intake, but Does Not Affect Relapse-like Drinking Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology. Mar, 2012 | Pubmed ID: 22113086 Design and Generation of Gene-Targeting Vectors Current Protocols in Mouse Biology. Mar, 2011 | Pubmed ID: 26068993 Forebrain CRF₁ Modulates Early-life Stress-programmed Cognitive Deficits The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2011 | Pubmed ID: 21940453 Requirement of the RNA-editing Enzyme ADAR2 for Normal Physiology in Mice The Journal of Biological Chemistry. May, 2011 | Pubmed ID: 21467037 Forebrain CRHR1 Deficiency Attenuates Chronic Stress-induced Cognitive Deficits and Dendritic Remodeling Neurobiology of Disease. Jun, 2011 | Pubmed ID: 21296667 Constitutive and Conditional RNAi Transgenesis in Mice Methods (San Diego, Calif.). Apr, 2011 | Pubmed ID: 21184828 Mouse Phenotyping Methods (San Diego, Calif.). Feb, 2011 | Pubmed ID: 20708688 A Powerful Transgenic Tool for Fate Mapping and Functional Analysis of Newly Generated Neurons BMC Neuroscience. Dec, 2010 | Pubmed ID: 21194452 Gene Knockdown in the Mouse Through RNAi Methods in Enzymology. 2010 | Pubmed ID: 20699152 CIN85 Regula La Endocitosis Del Receptor De Dopamina Y Gobierna El Comportamiento En Ratones The EMBO Journal. Jul, 2010 | Pubmed ID: 20551902 Phenotypic Annotation of the Mouse X Chromosome Genome Research. Aug, 2010 | Pubmed ID: 20548051 Central Deficiency of Corticotropin-releasing Hormone Receptor Type 1 (CRH-R1) Abolishes Effects of CRH on NREM but Not on REM Sleep in Mice Sleep. Apr, 2010 | Pubmed ID: 20394311 The Specific Role of Histone Deacetylase 2 in Adult Neurogenesis Neuron Glia Biology. May, 2010 | Pubmed ID: 20388229 Microphthalmia, Parkinsonism, and Enhanced Nociception in Pitx3 ( 416insG ) Mice Mammalian Genome : Official Journal of the International Mammalian Genome Society. Feb, 2010 | Pubmed ID: 20033184 Pez Cebra Mutantes Recompensa Revelan Nuevas Transcripciones Mediación De Los Efectos Conductuales De La Anfetamina Genome Biology. 2009 | Pubmed ID: 19646228 Dll1 Haploinsufficiency in Adult Mice Leads to a Complex Phenotype Affecting Metabolic and Immunological Processes PloS One. Jun, 2009 | Pubmed ID: 19562077 Mitochondrial Glutathione Peroxidase 4 Disruption Causes Male Infertility FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Sep, 2009 | Pubmed ID: 19417079 Neurological Phenotype and Reduced Lifespan in Heterozygous Tim23 Knockout Mice, the First Mouse Model of Defective Mitochondrial Import Biochimica Et Biophysica Acta. May, 2009 | Pubmed ID: 19111522 Activation of ERK/MAPK in the Lateral Amygdala of the Mouse is Required for Acquisition of a Fear-potentiated Startle Response Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology. Jan, 2009 | Pubmed ID: 18432190 Pleiotropic Effects in Eya3 Knockout Mice BMC Developmental Biology. Dec, 2008 | Pubmed ID: 19102749 Novel Caspase-suicide Proteins for Tamoxifen-inducible Apoptosis Genesis (New York, N.Y. : 2000). Oct, 2008 | Pubmed ID: 18802959 Novel Allele of Crybb2 in the Mouse and Its Expression in the Brain Investigative Ophthalmology & Visual Science. Apr, 2008 | Pubmed ID: 18385073 Un protocolo adaptable para cadena montaje gRNA clonación (fantasía STAgR) Christopher T. Breunig1,2, Andrea M. Neuner1,2, Jessica Giehrl-Schwab3, Wolfgang Wurst3, Magdalena Götz2,4, Stefan H. Stricker1,2 1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center JoVE 58556 Genetics
Un protocolo adaptable para cadena montaje gRNA clonación (fantasía STAgR) Christopher T. Breunig1,2, Andrea M. Neuner1,2, Jessica Giehrl-Schwab3, Wolfgang Wurst3, Magdalena Götz2,4, Stefan H. Stricker1,2 1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center JoVE 58556 Genetics