Yasushi Hiraoka Advanced ICT Research Institute Kobe National Institute of Information and Communications Technology Biography Publications Institution JoVE Articles Yasushi Hiraoka has not added a biography. If you are Yasushi Hiraoka and would like to personalize this page please email our Author Liaison for assistance. Publications Lem2 and Lnp1 Maintain the Membrane Boundary Between the Nuclear Envelope and Endoplasmic Reticulum Communications Biology. Jun, 2020 | Pubmed ID: 32483293 Phase Separation Drives Pairing of Homologous Chromosomes Current Genetics. Apr, 2020 | Pubmed ID: 32285141 Maintenance of Meiotic Crossover Against Reduced Double-strand Break Formation in Fission Yeast Lacking Histone H2A.Z Gene. Jun, 2020 | Pubmed ID: 32222534 Improved Methods for Preparing the Telomere Tethering Complex Bqt1-Bqt2 for Structural Studies The Protein Journal. 04, 2020 | Pubmed ID: 32140970 Intracellular ATP Levels Influence Cell Fates in Dictyostelium Discoideum Differentiation Genes to Cells : Devoted to Molecular & Cellular Mechanisms. May, 2020 | Pubmed ID: 32125743 Roles of Remote and Contact Forces in Epithelial Cell Structure Formation Biophysical Journal. Mar, 2020 | Pubmed ID: 32097624 Human Ebp1 Rescues the Synthetic Lethal Growth of Fission Yeast Cells Lacking Cdb4 and Nup184 Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Apr, 2020 | Pubmed ID: 32049412 The Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture Through Genic and Subtelomeric Functions Molecular Cell. 02, 2020 | Pubmed ID: 31837996 Chromosome-associated RNA-protein Complexes Promote Pairing of Homologous Chromosomes During Meiosis in Schizosaccharomyces Pombe Nature Communications. 12, 2019 | Pubmed ID: 31811152 Regulation of Ectopic Heterochromatin-mediated Epigenetic Diversification by the JmjC Family Protein Epe1 PLoS Genetics. 06, 2019 | Pubmed ID: 31206516 Nuclear Formation Induced by DNA-conjugated Beads in Living Fertilised Mouse Egg Scientific Reports. 06, 2019 | Pubmed ID: 31186495 Asymmetrical Localization of Nup107-160 Subcomplex Components Within the Nuclear Pore Complex in Fission Yeast PLoS Genetics. 06, 2019 | Pubmed ID: 31170156 Histone H2A Insufficiency Causes Chromosomal Segregation Defects Due to Anaphase Chromosome Bridge Formation at RDNA Repeats in Fission Yeast Scientific Reports. 05, 2019 | Pubmed ID: 31073221 The Very-long-chain Fatty Acid Elongase Elo2 Rescues Lethal Defects Associated with Loss of the Nuclear Barrier Function in Fission Yeast Cells Journal of Cell Science. 05, 2019 | Pubmed ID: 30975915 Roles of Nup133, Nup153 and Membrane Fenestrations in Assembly of the Nuclear Pore Complex at the End of Mitosis Genes to Cells : Devoted to Molecular & Cellular Mechanisms. May, 2019 | Pubmed ID: 30821042 Nuclear Localization Signal Targeting to Macronucleus and Micronucleus in Binucleated Ciliate Tetrahymena Thermophila Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Jul, 2018 | Pubmed ID: 29882620 Exportin Crm1 is Repurposed As a Docking Protein to Generate Microtubule Organizing Centers at the Nuclear Pore ELife. 05, 2018 | Pubmed ID: 29809148 Accurate and Fiducial-marker-free Correction for Three-dimensional Chromatic Shift in Biological Fluorescence Microscopy Scientific Reports. 05, 2018 | Pubmed ID: 29765093 The Conserved Histone Variant H2A.Z Illuminates Meiotic Recombination Initiation Current Genetics. Oct, 2018 | Pubmed ID: 29549582 Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 29423851 Lem2 is Retained at the Nuclear Envelope Through Its Interaction with Bqt4 in Fission Yeast Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Mar, 2018 | Pubmed ID: 29292846 The Histone Variant H2A.Z Promotes Initiation of Meiotic Recombination in Fission Yeast Nucleic Acids Research. 01, 2018 | Pubmed ID: 29145618 Ser7 of RNAPII-CTD Facilitates Heterochromatin Formation by Linking NcRNA to RNAi Proceedings of the National Academy of Sciences of the United States of America. 12, 2017 | Pubmed ID: 29237752 Visualization of a Specific Genome Locus by the /LacI-GFP System Cold Spring Harbor Protocols. Oct, 2017 | Pubmed ID: 28733400 Microscopic Observation of Living Cells Stained with Fluorescent Probes Cold Spring Harbor Protocols. Oct, 2017 | Pubmed ID: 28733393 Function of Nuclear Membrane Proteins in Shaping the Nuclear Envelope Integrity During Closed Mitosis Journal of Biochemistry. Jun, 2017 | Pubmed ID: 28398483 Compositionally Distinct Nuclear Pore Complexes of Functionally Distinct Dimorphic Nuclei in the Ciliate Journal of Cell Science. 05, 2017 | Pubmed ID: 28386019 Fission Yeast APC/C Activators Slp1 and Fzr1 Sequentially Trigger Two Consecutive Nuclear Divisions During Meiosis FEBS Letters. 04, 2017 | Pubmed ID: 28245054 Lipid Droplet Dynamics During Sporulation and Their Role in Spore Survival Biology Open. Feb, 2017 | Pubmed ID: 28011631 In vitro Reconstitution and Biochemical Analyses of the Schizosaccharomyces Pombe Nucleosome Biochemical and Biophysical Research Communications. Jan, 2017 | Pubmed ID: 27890612 Spatial Organization of the Schizosaccharomyces Pombe Genome Within the Nucleus Yeast (Chichester, England). 02, 2017 | Pubmed ID: 27766670 Borna Disease Virus Assembles Porous Cage-like Viral Factories in the Nucleus The Journal of Biological Chemistry. Dec, 2016 | Pubmed ID: 27803166 Meiotic Cohesin Subunits RAD21L and REC8 Are Positioned at Distinct Regions Between Lateral Elements and Transverse Filaments in the Synaptonemal Complex of Mouse Spermatocytes The Journal of Reproduction and Development. Dec, 2016 | Pubmed ID: 27665783 A Genetically Encoded Probe for Live-Cell Imaging of H4K20 Monomethylation Journal of Molecular Biology. 10, 2016 | Pubmed ID: 27534817 Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation PLoS Genetics. 06, 2016 | Pubmed ID: 27341616 Inner Nuclear Membrane Protein Lem2 Augments Heterochromatin Formation in Response to Nutritional Conditions Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Aug, 2016 | Pubmed ID: 27334362 Depletion of Autophagy Receptor P62/SQSTM1 Enhances the Efficiency of Gene Delivery in Mammalian Cells FEBS Letters. Aug, 2016 | Pubmed ID: 27317902 Uniquely Designed Nuclear Structures of Lower Eukaryotes Current Opinion in Cell Biology. 06, 2016 | Pubmed ID: 26963276 A Cohesin-based Structural Platform Supporting Homologous Chromosome Pairing in Meiosis Current Genetics. Aug, 2016 | Pubmed ID: 26856595 Shugoshin Forms a Specialized Chromatin Domain at Subtelomeres That Regulates Transcription and Replication Timing Nature Communications. Jan, 2016 | Pubmed ID: 26804021 Histone H3K36 Trimethylation is Essential for Multiple Silencing Mechanisms in Fission Yeast Nucleic Acids Research. 05, 2016 | Pubmed ID: 26792892 A Nucleoporin That Facilitates Meiotic Kinetochore Reorganization Cell Cycle (Georgetown, Tex.). 2016 | Pubmed ID: 26727711 Meiotic Cohesin-based Chromosome Structure is Essential for Homologous Chromosome Pairing in Schizosaccharomyces Pombe Chromosoma. Jun, 2016 | Pubmed ID: 26511279 Cellular Economy in Fission Yeast Cells Continuously Cultured with Limited Nitrogen Resources Scientific Reports. Oct, 2015 | Pubmed ID: 26486373 Nup132 Modulates Meiotic Spindle Attachment in Fission Yeast by Regulating Kinetochore Assembly The Journal of Cell Biology. Oct, 2015 | Pubmed ID: 26483559 Fission Yeast Meets a Legend in Kobe: Report of the Eighth International Fission Yeast Meeting Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Dec, 2015 | Pubmed ID: 26477989 Selective Autophagic Receptor P62 Regulates the Abundance of Transcriptional Coregulator ARIP4 During Nutrient Starvation Scientific Reports. 2015 | Pubmed ID: 26412716 Histone H4 Acetylation Required for Chromatin Decompaction During DNA Replication Scientific Reports. Jul, 2015 | Pubmed ID: 26223950 Highly Condensed Chromatins Are Formed Adjacent to Subtelomeric and Decondensed Silent Chromatin in Fission Yeast Nature Communications. Jul, 2015 | Pubmed ID: 26205977 Uncleavable Nup98-Nup96 is Functional in the Fission Yeast Schizosaccharomyces Pombe FEBS Open Bio. 2015 | Pubmed ID: 26137436 Recent Advancements in Structured-illumination Microscopy Toward Live-cell Imaging Microscopy (Oxford, England). Aug, 2015 | Pubmed ID: 26133185 Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins Scientific Reports. Jul, 2015 | Pubmed ID: 26132639 BAF is a Cytosolic DNA Sensor That Leads to Exogenous DNA Avoiding Autophagy Proceedings of the National Academy of Sciences of the United States of America. Jun, 2015 | Pubmed ID: 25991860 Biased Assembly of the Nuclear Pore Complex is Required for Somatic and Germline Nuclear Differentiation in Tetrahymena Journal of Cell Science. May, 2015 | Pubmed ID: 25788697 Fission Yeast Scp3 Potentially Maintains Microtubule Orientation Through Bundling PloS One. 2015 | Pubmed ID: 25767875 Chromosomes Rein Back the Spindle Pole Body During Horsetail Movement in Fission Yeast Meiosis Cell Structure and Function. 2014 | Pubmed ID: 24954111 A Method of Correlative Light and Electron Microscopy for Yeast Cells Micron (Oxford, England : 1993). Jun, 2014 | Pubmed ID: 24792447 Puromycin Resistance Gene As an Effective Selection Marker for Ciliate Tetrahymena Gene. Jan, 2014 | Pubmed ID: 24185080 Monoclonal Antibodies Recognize Gly-leu-phe-gly Repeat of Nucleoporin Nup98 of Tetrahymena, Yeasts, and Humans Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. Apr, 2013 | Pubmed ID: 23607342 Lamin B Receptor Recognizes Specific Modifications of Histone H4 in Heterochromatin Formation The Journal of Biological Chemistry. Dec, 2012 | Pubmed ID: 23100253 Meiosis-specific Noncoding RNA Mediates Robust Pairing of Homologous Chromosomes in Meiosis Science (New York, N.Y.). May, 2012 | Pubmed ID: 22582262 Early Entry and Deformation of Macropinosomes Correlates with High Efficiency of Decaarginine-polyethylene Glycol-lipid-mediated Gene Delivery The Journal of Gene Medicine. Apr, 2012 | Pubmed ID: 22351041 Exportin 4 Interacts with Sox9 Through the HMG Box and Inhibits the DNA Binding of Sox9 PloS One. 2011 | Pubmed ID: 21991335 Inner Nuclear Membrane Protein Ima1 is Dispensable for Intranuclear Positioning of Centromeres Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Oct, 2011 | Pubmed ID: 21880100 Aneuploidy Drives Genomic Instability in Yeast Science (New York, N.Y.). Aug, 2011 | Pubmed ID: 21852501 Symmetry, Asymmetry, and Kinetics of Silencing Establishment in Saccharomyces Cerevisiae Revealed by Single-cell Optical Assays Proceedings of the National Academy of Sciences of the United States of America. Jan, 2011 | Pubmed ID: 21262833 Spatiotemporal Regulations of Wee1 at the G2/M Transition Molecular Biology of the Cell. Mar, 2011 | Pubmed ID: 21233285 Nuclear Envelope Attachment is Not Necessary for Telomere Function in Fission Yeast Nucleus (Austin, Tex.). Nov-Dec, 2010 | Pubmed ID: 21327090 In Vivo Evidence for the Fibrillar Structures of Sup35 Prions in Yeast Cells The Journal of Cell Biology. Jul, 2010 | Pubmed ID: 20643880 Nucleoporin Nup98: a Gatekeeper in the Eukaryotic Kingdoms Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Jun, 2010 | Pubmed ID: 20545767 From Meiosis to Postmeiotic Events: Alignment and Recognition of Homologous Chromosomes in Meiosis The FEBS Journal. Feb, 2010 | Pubmed ID: 20015081 Artificial Induction of Autophagy Around Polystyrene Beads in Nonphagocytic Cells Autophagy. Jan, 2010 | Pubmed ID: 19901555 Membrane Proteins Bqt3 and -4 Anchor Telomeres to the Nuclear Envelope to Ensure Chromosomal Bouquet Formation The Journal of Cell Biology. Nov, 2009 | Pubmed ID: 19948484 The SUN Rises on Meiotic Chromosome Dynamics Developmental Cell. Nov, 2009 | Pubmed ID: 19922865 Transcriptional Suppression by Transient Recruitment of ARIP4 to Sumoylated Nuclear Receptor Ad4BP/SF-1 Molecular Biology of the Cell. Oct, 2009 | Pubmed ID: 19692572 Distinctive Responses to Nitrogen Starvation in the Dominant Active Mutants of the Fission Yeast Rheb GTPase Genetics. Oct, 2009 | Pubmed ID: 19620394 Two Distinct Repeat Sequences of Nup98 Nucleoporins Characterize Dual Nuclei in the Binucleated Ciliate Tetrahymena Current Biology : CB. May, 2009 | Pubmed ID: 19375312 Codon Usage Bias is Correlated with Gene Expression Levels in the Fission Yeast Schizosaccharomyces Pombe Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Apr, 2009 | Pubmed ID: 19335619 Localization of Gene Products Using a Chromosomally Tagged GFP-fusion Library in the Fission Yeast Schizosaccharomyces Pombe Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Feb, 2009 | Pubmed ID: 19170768 Spindle Checkpoint Activation at Meiosis I Advances Anaphase II Onset Via Meiosis-specific APC/C Regulation The Journal of Cell Biology. Jul, 2008 | Pubmed ID: 18644893 Live Cell Imaging and Electron Microscopy Reveal Dynamic Processes of BAF-directed Nuclear Envelope Assembly Journal of Cell Science. Aug, 2008 | Pubmed ID: 18628300 Live Observation of Forespore Membrane Formation in Fission Yeast Molecular Biology of the Cell. Aug, 2008 | Pubmed ID: 18550796 Gene Expression and Distribution of Swi6 in Partial Aneuploids of the Fission Yeast Schizosaccharomyces Pombe Cell Structure and Function. 2007 | Pubmed ID: 18285637 Another Way to Move Chromosomes Chromosoma. Dec, 2007 | Pubmed ID: 17639451 Reconstruction of the Kinetochore: a Prelude to Meiosis Cell Division. Jun, 2007 | Pubmed ID: 17550626 Nuclear Localization of Barrier-to-autointegration Factor is Correlated with Progression of S Phase in Human Cells Journal of Cell Science. Jun, 2007 | Pubmed ID: 17519288 Reconstruction of the Kinetochore During Meiosis in Fission Yeast Schizosaccharomyces Pombe Molecular Biology of the Cell. Dec, 2006 | Pubmed ID: 17035632 Meiotic Cohesins Modulate Chromosome Compaction During Meiotic Prophase in Fission Yeast The Journal of Cell Biology. Aug, 2006 | Pubmed ID: 16893973 Meiotic Proteins Bqt1 and Bqt2 Tether Telomeres to Form the Bouquet Arrangement of Chromosomes Cell. Apr, 2006 | Pubmed ID: 16615890 The Carboxy-terminus of Alp4 Alters Microtubule Dynamics to Induce Oscillatory Nuclear Movement Led by the Spindle Pole Body in Schizosaccharomyces Pombe Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Apr, 2006 | Pubmed ID: 16611238 Modulation of Alp4 Function in Schizosaccharomyces Pombe Induces Novel Phenotypes That Imply Distinct Functions for Nuclear and Cytoplasmic Gamma-tubulin Complexes Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Apr, 2006 | Pubmed ID: 16611237 Dissociation of the Nuf2-Ndc80 Complex Releases Centromeres from the Spindle-pole Body During Meiotic Prophase in Fission Yeast Molecular Biology of the Cell. May, 2005 | Pubmed ID: 15728720 Cadherin Activity is Required for Activity-induced Spine Remodeling The Journal of Cell Biology. Dec, 2004 | Pubmed ID: 15569714 Meiosis Induced by Inactivation of Pat1 Kinase Proceeds with Aberrant Nuclear Positioning of Centromeres in the Fission Yeast Schizosaccharomyces Pombe Genes to Cells : Devoted to Molecular & Cellular Mechanisms. Aug, 2004 | Pubmed ID: 15298676 High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon Atsushi Matsuda1,2, Takako Koujin1, Lothar Schermelleh3, Tokuko Haraguchi1,2, Yasushi Hiraoka1,2 1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford JoVE 60800 Biology
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon Atsushi Matsuda1,2, Takako Koujin1, Lothar Schermelleh3, Tokuko Haraguchi1,2, Yasushi Hiraoka1,2 1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford JoVE 60800 Biology