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Mouse Neonatal Brain Micro-CT Scanning and Image Processing

May 19, 2023
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Author Spotlight: High-Resolution Imaging of Mouse Neonate Brains – A Micro-CT Protocol with Lugol's Solution Contrast Agent

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Murine Neonatal Brain Sample Fixation and Staining for Micro-CT Scanning

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Processing Micro-CT Scanned Image of Stained Murine Neonatal Brain Sample

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1Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos (ENyS, CONICET-UNAJ-HEC), 2Facultad de Ciencias Médicas,Universidad Nacional de La Plata, 3Facultad de Ciencias Naturales y Museo,Universidad Nacional de La Plata, 4Department of Cell Biology and Anatomy, Cumming School of Medicine,University of Calgary

Summary

Automatic Translation

This study describes the steps for obtaining high-resolution images of neonatal mouse brains by combining micro-computed tomography (micro-CT) and a contrast agent in ex vivo samples. We describe basic morphometric analyses to quantify brain size and shape in these images.

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