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check_url/65215?article_type=b
Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
July 07, 2023
check_url/65215?article_type=b
一种用于FACS分离的小鼠卫星细胞的CUT&RUN分析的有效方案
02:40 min
•
check_url/65215?article_type=b
通过荧光激活细胞分选制备用于分离卫星细胞的小鼠肌肉细胞悬液
03:36 min
•
check_url/65215?article_type=b
使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放
04:25 min
•
Kamar Ghaibour*
1
,
Joe Rizk*
1
,
Claudine Ebel
,
Tao Ye
,
Muriel Philipps
,
Valérie Schreiber
,
Daniel Metzger
,
Delphine Duteil
1
CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258
,
Université de Strasbourg
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这里介绍的是一种有效的方案,用于小鼠肢体肌肉卫星细胞的荧光激活细胞分选(FACS)分离,适用于研究肌肉纤维中的转录调控,方法是在靶标下切割并使用核酸酶(CUT&RUN)释放。
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