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Locus-Specific Chromatin Isolation from Yeast Cells

November 17, 2023
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Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol (Video) | JoVE

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TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae (Video) | JoVE

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1Institute of Epigenetics and Stem Cells,Helmholtz Zentrum München

Summary

Automatic Translation

This protocol presents a locus-specific chromatin isolation method based on site-specific recombination to purify a single-copy gene locus of interest in its native chromatin context from budding yeast, Saccharomyces cerevisiae.

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