Assessment of Acinetobacter Biofilm Formation and Characterization of Biofilms

August 04, 2023

A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization (Video) | JoVE

01:29 min

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Preparation of Bacterial Inoculum for Acinetobacter Biofilm Formation (Video) | JoVE

01:38 min

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Acinetobacter Biofilm Quantification Using Crystal Violet (Video) | JoVE

01:47 min

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Assessing Acinetobacter Biofilm Viability Count (Video) | JoVE

01:58 min

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Acinetobacter Biofilm Visualization by Confocal Laser Scanning Microscopy (CLSM)

01:20 min

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Joo-Sung Kim², Jihoon Lim

¹Korea Food Research Institute, ²Department of Food Biotechnology,Korea University of Science and Technology, ³Advanced Radiation Technology Institute,Korea Atomic Energy Research Institute

Summary

This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter.