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check_url/65521?article_type=b
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
June 30, 2023
check_url/65521?article_type=b
骨髄由来マクロファージ由来の微小細胞外小胞ペプチドの同定
01:19 min
•
check_url/65521?article_type=b
示差超遠心法による微小細胞外小胞(sEV)の単離
04:25 min
•
check_url/65521?article_type=b
単離された小細胞外小胞(sEV)からのペプチドの抽出
03:04 min
•
Jiale Cheng
2
,
Jiong Zhu
2
,
Yujiao Liu
3
,
Chen Yang
3
,
Yuehui Zhang
,
Yuchen Liu
,
Chaozhi Jin
,
Jian Wang
2
1
School of Basic Medical Sciences
,
Anhui Medical University
,
2
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing)
,
Beijing Institute of Lifeomics
,
3
School of Life Sciences
,
Hebei University
Summary
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このプロトコルは、示差超遠心によってマクロファージから小さな細胞外小胞を単離し、質量分析による同定のためにペプチドームを抽出する手順を説明しています。
Article