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De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20th, 2023
1,2, 1,2, 1,2,3, 4,5, 6, 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary
Automatic Translation

Viene presentato un protocollo unico e completo per generare cellule dendritiche (mo-DC) derivate da monociti umani desialilati da cellule mononucleate isolate del sangue periferico (PBMC) utilizzando un trattamento con sialidasi. Inoltre, vengono descritti metodi per valutare la caratterizzazione fenotipica e funzionale delle mo-DC e valutare come il trattamento con sialidasi migliora il livello di maturazione delle mo-DC.

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