Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

February 2nd, 2024

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

01:29 min

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In vitro humane pluripotente stamceldifferentiatie in insulineproducerende alvleeskliercellen

05:06 min

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Ines Cherkaoui¹, Qian Du², Dieter Egli², Shivani Misra^1,3, Guy A. Rutter^1,4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, ³Department of Diabetes, Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine, Université de Montréal, ⁵Lee Kong Chian Imperial Medical School, Nanyang Technological University

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Dit artikel presenteert een protocol voor gerichte differentiatie en functionele analyse van β-celachtige cellen. We beschrijven optimale kweekomstandigheden en doorgangen voor menselijke pluripotente stamcellen voordat insulineproducerende pancreascellen worden gegenereerd. De differentiatie in zes fasen vordert van definitieve endodermvorming naar functionele β-celachtige cellen die insuline afscheiden als reactie op glucose.

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Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

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