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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

July 28th, 2023

Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues (Video) | JoVE

Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues (Video) | JoVE

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Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE

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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

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Lucas Stalder¹, Fabian Koechl¹, Kerstin Hahn¹, Marc Sultan¹, Megana K. Prasad¹

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel

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המחקר מתאר פרוטוקול פשוט, מהיר ואוטומטי חלקית לבידוד גרעינים איכותיים מרקמות יונקים קפואים לצורך ריצוף RNA של גרעינים בודדים במורד הזרם.

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