Mapping the Tumor Immune Landscape: An In-depth Multiplex IHC Analysis Protocol

August 18, 2023

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Mark A. J. Gorris², Evgenia Martynova*^1,3, Mark W. D. Sweep*^1,4, Iris A. E. van der Hoorn⁵, Shabaz Sultan³, Mike J. D. E. Claassens, Lieke L. van der Woude^2,6, Kiek Verrijp^2,6, Carl G. Figdor³, Johannes Textor³, I. Jolanda M. de Vries

¹Department of Medical BioSciences,Radboudumc, ²Division of Immunotherapy, Oncode Institute,Radboudumc, ³Data Science, Institute for Computing and Information Sciences,Radboud University, ⁴Department of Medical Oncology,Radboudumc, ⁵Department of Pulmonary Diseases,Radboudumc, ⁶Department of Pathology,Radboudumc

Summary

This protocol describes in detail how immune cell characterization of the tumor microenvironment using multiplex immunohistochemistry is carried out.

Article