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Characterizing Surface Antigens and Proliferation Ability in hiPSC-Driving MSCs
October 20, 2023
人诱导多能干细胞分化为间充质基质细胞的两种代表性方法比较
01:06 min
•
通过EB形成和单层培养从hiPSCs中分化MSCss
05:21 min
•
Fangqin Wang
,
Langping Gao
3
,
Xudong Fu
,
Qintao Yan
,
Lidan Hu
2
,
Jianhua Mao
3
1
National Clinical Research Center for Child Health
,
The Children’s Hospital, Zhejiang University School of Medicine
,
2
Department of Nephrology
,
The Children’s Hospital, Zhejiang University School of Medicine
,
3
Zhejiang University School of Medicine
,
4
Liangzhu Laboratory
,
Zhejiang University
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该协议描述并比较了将hiPSC分化为间充质基质细胞(MSCs)的两种代表性方法。单层法的特点是成本更低,操作更简单,更容易成骨分化。胚状体(EBs)方法的特点是时间消耗更短。
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