Revealing Murine Pulmonary Veins Myocardial Architecture

November 21, 2023

小鼠肺静脉心肌套的显微解剖和免疫荧光染色

用肺静脉隔离小鼠左心房（LA）的显微解剖方案

小鼠肺静脉心肌套管的组织包埋和免疫荧光染色

Hannes E. Villgrater*^1,2,3, Ruibing Xia*^1,2,3, Aparna Sharma Chivukula^2,3, Philipp Tomsits*^1,2,3, Sebastian Clauss*^1,2,3,4

¹University Hospital Munich, Department of Medicine I,Ludwig-Maximilians-University Munich (LMU), ²Walter Brendel Center of Experimental Medicine (WBex),LMU Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ⁴Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU),LMU Munich

Summary

该方案展示了小鼠肺静脉的显微镜引导分离和免疫荧光染色。我们制备包含左心房、肺静脉和相应肺的组织样本，并对它们进行心脏肌钙蛋白 T 和连接蛋白 43 染色。

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