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Monitoring Brain Metabolite Transport in Drosophila Using FRET Sensors

October 27, 2023
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Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors

01:53 min
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Culturing Drosophila Third Instar Larvae for Brain Metabolite Studies

00:58 min
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Dissection of Third Instar Drosophila Larva for Brain Metabolite Imaging

04:31 min
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, , , , ,
1Biomedical Neuroscience Institute,Universidad de Chile, 2Advanced Scientific Equipment Network (REDECA), Faculty of Medicine,Universidad de Chile, 3Department of Neuroscience, Faculty of Medicine,Universidad de Chile

Summary

Automatic Translation

Here we present a protocol to visualize the transport of monocarboxylates, glucose, and ATP in glial cells and neurons using genetically encoded Förster resonance energy transfer-based sensors in an ex-vivo Drosophila larval brain preparation.

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