Mechanical Fatigue Testing of RBCs by Amplitude-Modulated Electrodeformation

October 13, 2023

Studying Biomechanics of Circulating Cells by Modulating Their Electrodeformation Behavior (Video) | JoVE

01:30 min

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Preparation of Dielectrophoresis Medium and Electrodeformation Prime Medium (Video) | JoVE

01:06 min

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Preparation of the Microfluidic Device for Electrodeformation of RBCs (Video) | JoVE

03:40 min

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Preparation of Human RBC Suspension for Electrodeformation Experiment (Video) | JoVE

01:20 min

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Preparing the Electrodeformation Setup and Performing Fatigue Testing on Human RBC (Video) | JoVE

02:24 min

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Darryl Dieujuste, Adeleh Kazemi Alamouti, Hongyuan Xu, E Du²

¹Department of Ocean and Mechanical Engineering,Florida Atlantic University, ²Center for SMART Health,Florida Atlantic University

Summary

Presented here is a protocol for mechanical fatigue testing in the case of human red blood cells using an amplitude-modulated electrodeformation approach. This general approach can be used to measure the systematic changes in morphological and biomechanical characteristics of biological cells in a suspension from cyclic deformation.