1European Laboratory for Non-linear Spectroscopy (LENS),University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine,University of Florence, 3Department of Physics,University of Pisa, 4National Research Council – National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy,University of Florence, 6Department of Biology,University of Florence
The present protocol provides a step-by-step procedure for rapid and simultaneous optical clearing, muti-round labeling, and 3D volumetric reconstruction of tens of postmortem human brain sections by combining the (SWITCH – H2O2 – Antigen Retrieval – 2,2′-thiodiethanol [TDE]) SHORT tissue transformation technique with light-sheet fluorescence microscopy imaging in a routinely high-throughput protocol.
