Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living <em>Drosophila</em> Embryos <em>via</em> Fluorescent Antibody Injection

Qihong Zheng; Xiaoxiang Xiang; Yan Yan; Huapeng  H. Yu

doi:10.3791/66080

Enhancing Protein Visualization with Antibody-Mediated Live Imaging

January 19, 2024

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01:35 min

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Fluorescent Labeling of Antibodies for Imaging Low Abundance Proteins in Drosophila (Video) | JoVE

00:49 min

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Preparation and Alignment of Drosophila Embryos for Injecting Fluorescently Labeled Antibodies (Video) | JoVE

02:42 min

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Injecting Fluorescently Labeled Antibodies in Live Drosophila Embryos and Imaging of Low Abundance Proteins (Video) | JoVE

02:21 min

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Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan³, Huapeng H. Yu

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Summary

This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.