1Keck School of Medicine,University of Southern California, 2The Vision Center, Department of Surgery,Children’s Hospital Los Angeles, 3The Saban Research Institute,Children’s Hospital Los Angeles, 4USC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine,University of Southern California, 5Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research,University of Southern California, 6Tina and Rick Caruso Department of Otolaryngology-Head and Neck Surgery,University of Southern California
This protocol describes a reliable method for obtaining high-quality cryosections of whole rabbit eyes. It details rabbit eye dissection, fixation, embedding, and sectioning procedures, which may be easily adapted for use in any study utilizing immunohistochemistry in larger eyes.
