Single Cell and Nuclei Extraction from Human Brain Organoids

March 29, 2024

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic³, Anastasiya Boltengagen, Agnieszka Rybak-Wolf³, Nikolaus Rajewsky²

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Summary

Here, we introduce a comprehensive protocol for the generation and downstream analysis of human brain organoids using single-cell and single-nucleus RNA sequencing.

Single Cell and Nuclei Extraction from Human Brain Organoids

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Summary

Article