Rearing Axenic <em>Delia antiqua</em> with Half-Fermented Sterile Diets

Xin Cao; Qingxia Liang; Miaomiao Li; Xiaoqing Wu; Susu Fan; Xinjian Zhang; Fangyuan Zhou; Zhiqing Zhao

doi:10.3791/66259

Studying Development of Axenic Delia antiqua and Ensuring Bacteria-Free Growth

December 22, 2023

Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development

01:14 min

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Preparation of Sterile Diets Incorporating Scallions and Insect Larvae for the Rearing of Delia antiqua

02:53 min

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Collecting Axenic Eggs and Rearing of Axenic Delia antiqua Larvae on Half-Fermented Sterile Diets

01:54 min

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Xin Cao, Qingxia Liang, Miaomiao Li, Xiaoqing Wu, Susu Fan, Xinjian Zhang, Fangyuan Zhou, Zhiqing Zhao

¹Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute,Qilu University of Technology (Shandong Academy of Sciences), ²Agriculture and Rural Bureau of Changqing District

Summary

A simple procedure for rearing axenic Delia antiqua with half-fermented sterile diets is described. Only one Wolbachia strain was detected in each instar of axenic D. antiqua using PCR.

Studying Development of Axenic Delia antiqua and Ensuring Bacteria-Free Growth

Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development

Preparation of Sterile Diets Incorporating Scallions and Insect Larvae for the Rearing of Delia antiqua

Collecting Axenic Eggs and Rearing of Axenic Delia antiqua Larvae on Half-Fermented Sterile Diets

Summary

Article