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Cell-Free Expression of Membrane Proteins in Giant Unilamellar Vesicles

March 8th, 2024
*1,2, *3, 2,3,4,5
1Neuroscience Program, University of Michigan, Ann Arbor, 2Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, 3Department of Mechanical Engineering, University of Michigan, Ann Arbor, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor
* These authors contributed equally

Summary
Automatic Translation

Dieses Protokoll beschreibt das invertierte Emulsionsverfahren, das zur Verkapselung eines zellfreien Expressionssystems (CFE) in einem riesigen unilamellären Vesikel (GUV) zur Untersuchung der Synthese und des Einbaus eines Modellmembranproteins in die Lipiddoppelschicht verwendet wird.

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