Efficient Extraction and Purification of GFP-His from N. benthamiana Leaves

July 05, 2024

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Huiying Ji², Tianhao Zhang, Han Li³, Chunyu Wang², Rongxiang Fang, Lili Zhang^2,4, Yan Huo

¹Shandong Academy of Medical Sciences,Shandong First Medical University, ²State Key Laboratory of Plant Genomics, Institute of Microbiology,Chinese Academy of Sciences, ³College of Veterinary Medicine,Shanxi Agricultural University, ⁴Beijing Life Science Academy

Summary

The purification of recombinant proteins from plant systems is usually challenged by plant proteins. This work provides a method to effectively extract and purify a secreted recombinant protein from the apoplast of Nicotiana benthamiana.

Efficient Extraction and Purification of GFP-His from N. benthamiana Leaves

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Summary

Article