Judy Lieberman Cellular and Molecular Medicine Program Boston Children’s Hospital and Harvard Medical School Biography Publications Institution JoVE Articles Judy Lieberman has not added a biography. If you are Judy Lieberman and would like to personalize this page please email our Author Liaison for assistance. Publications An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MiRNAs and MRNAs It Regulates The Journal of Biological Chemistry. Jun, 2015 | Pubmed ID: 26045559 Apoptosis Triggers Specific, Rapid, and Global MRNA Decay with 3' Uridylated Intermediates Degraded by DIS3L2 Cell Reports. May, 2015 | Pubmed ID: 25959823 Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells PloS One. 2015 | Pubmed ID: 25875117 The Rab2A GTPase Promotes Breast Cancer Stem Cells and Tumorigenesis Via Erk Signaling Activation Cell Reports. Apr, 2015 | Pubmed ID: 25818297 Harnessing RNA Interference for Therapy: the Silent Treatment JAMA. Mar 24-31, 2015 | Pubmed ID: 25803342 Dysregulation of MicroRNA Biogenesis and Gene Silencing in Cancer Science Signaling. Mar, 2015 | Pubmed ID: 25783160 G-quadruplex Structures Contribute to the Neuroprotective Effects of Angiogenin-induced TRNA Fragments Proceedings of the National Academy of Sciences of the United States of America. Dec, 2014 | Pubmed ID: 25404306 MiR-200-containing Extracellular Vesicles Promote Breast Cancer Cell Metastasis The Journal of Clinical Investigation. Dec, 2014 | Pubmed ID: 25401471 Sequencing of Captive Target Transcripts Identifies the Network of Regulated Genes and Functions of Primate-specific MiR-522 Cell Reports. Aug, 2014 | Pubmed ID: 25131211 Characterization of Dual PTEN and P53-targeting MicroRNAs Identifies MicroRNA-638/Dnm2 As a Two-hit Oncogenic Locus Cell Reports. Aug, 2014 | Pubmed ID: 25088422 Cytotoxic Cells Kill Intracellular Bacteria Through Granulysin-mediated Delivery of Granzymes Cell. Jun, 2014 | Pubmed ID: 24906149 Perforin: a Key Pore-forming Protein for Immune Control of Viruses and Cancer Sub-cellular Biochemistry. 2014 | Pubmed ID: 24798013 Prolyl Isomerase Pin1 Acts Downstream of MiR200c to Promote Cancer Stem-like Cell Traits in Breast Cancer Cancer Research. Jul, 2014 | Pubmed ID: 24786790 Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins Journal of Immunology (Baltimore, Md. : 1950). Jun, 2014 | Pubmed ID: 24771851 Distribution of Immune Cells in the Human Cervix and Implications for HIV Transmission American Journal of Reproductive Immunology (New York, N.Y. : 1989). Mar, 2014 | Pubmed ID: 24410939 Production of Highly Potent Recombinant SiRNAs in Escherichia Coli Nature Protocols. Dec, 2013 | Pubmed ID: 24177290 Arf-like GTPase Arl8b Regulates Lytic Granule Polarization and Natural Killer Cell-mediated Cytotoxicity Molecular Biology of the Cell. Dec, 2013 | Pubmed ID: 24088571 A Genome-wide SiRNA Screen Identifies Proteasome Addiction As a Vulnerability of Basal-like Triple-negative Breast Cancer Cells Cancer Cell. Aug, 2013 | Pubmed ID: 23948298 Noncoding RNAs and Cancer Cell. Mar, 2013 | Pubmed ID: 23781554 Durable Knockdown and Protection from HIV Transmission in Humanized Mice Treated with Gel-formulated CD4 Aptamer-siRNA Chimeras Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2013 | Pubmed ID: 23629001 Live or Let Die: Posttranscriptional Gene Regulation in Cell Stress and Cell Death Immunological Reviews. May, 2013 | Pubmed ID: 23550650 Micromanaging Hepatitis C Virus The New England Journal of Medicine. May, 2013 | Pubmed ID: 23534545 Efficient and Specific Gene Knockdown by Small Interfering RNAs Produced in Bacteria Nature Biotechnology. Apr, 2013 | Pubmed ID: 23475073 Identification of Regulators of the Innate Immune Response to Cytosolic DNA and Retroviral Infection by an Integrative Approach Nature Immunology. Feb, 2013 | Pubmed ID: 23263557 SAMHD1 Does It Again, Now in Resting T Cells Nature Medicine. Nov, 2012 | Pubmed ID: 23135508 Alterations in RNA Processing During Immune-mediated Programmed Cell Death Proceedings of the National Academy of Sciences of the United States of America. May, 2012 | Pubmed ID: 22589304 Targeted Delivery of PLK1-siRNA by ScFv Suppresses Her2+ Breast Cancer Growth and Metastasis Science Translational Medicine. Apr, 2012 | Pubmed ID: 22517885 Bone Morphogenetic Protein 4 Promotes Vascular Smooth Muscle Contractility by Activating MicroRNA-21 (miR-21), Which Down-regulates Expression of Family of Dedicator of Cytokinesis (DOCK) Proteins The Journal of Biological Chemistry. Feb, 2012 | Pubmed ID: 22158624 Capture of MicroRNA-bound MRNAs Identifies the Tumor Suppressor MiR-34a As a Regulator of Growth Factor Signaling PLoS Genetics. Nov, 2011 | Pubmed ID: 22102825 Coding-independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous MRNAs Cell. Oct, 2011 | Pubmed ID: 22000013 Conserved Regulation of P53 Network Dosage by MicroRNA-125b Occurs Through Evolving MiRNA-target Gene Pairs PLoS Genetics. Sep, 2011 | Pubmed ID: 21935352 Prime-boost Vaccination with Heterologous Live Vectors Encoding SIV Gag and Multimeric HIV-1 Gp160 Protein: Efficacy Against Repeated Mucosal R5 Clade C SHIV Challenges Vaccine. Aug, 2011 | Pubmed ID: 21693155 Perforin Pores in the Endosomal Membrane Trigger the Release of Endocytosed Granzyme B into the Cytosol of Target Cells Nature Immunology. Aug, 2011 | Pubmed ID: 21685908 Inhibition of HIV Transmission in Human Cervicovaginal Explants and Humanized Mice Using CD4 Aptamer-siRNA Chimeras The Journal of Clinical Investigation. Jun, 2011 | Pubmed ID: 21576818 HIV DNA is Heavily Uracilated, Which Protects It from Autointegration Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21576478 Prior Exposure to an Attenuated Listeria Vaccine Does Not Reduce Immunogenicity: Pre-clinical Assessment of the Efficacy of a Listeria Vaccine in the Induction of Immune Responses Against HIV Journal of Immune Based Therapies and Vaccines. 2011 | Pubmed ID: 21244649 Gaining a Foothold: How HIV Avoids Innate Immune Recognition Current Opinion in Immunology. Feb, 2011 | Pubmed ID: 21123040 Promise and Challenge of RNA Interference-based Therapy for Cancer Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology. Feb, 2011 | Pubmed ID: 21079135 Desperately Seeking MicroRNA Targets Nature Structural & Molecular Biology. Oct, 2010 | Pubmed ID: 20924405 The Cytosolic Exonuclease TREX1 Inhibits the Innate Immune Response to Human Immunodeficiency Virus Type 1 Nature Immunology. Nov, 2010 | Pubmed ID: 20871604 Granzyme A Activates Another Way to Die Immunological Reviews. May, 2010 | Pubmed ID: 20536557 Anatomy of a Murder: How Cytotoxic T Cells and NK Cells Are Activated, Develop, and Eliminate Their Targets Immunological Reviews. May, 2010 | Pubmed ID: 20536551 Isolation of Cytotoxic T Cell and NK Granules and Purification of Their Effector Proteins Current Protocols in Cell Biology / Editorial Board, Juan S. Bonifacino ... [et Al.]. Jun, 2010 | Pubmed ID: 20521234 Small RNAs Guide Hematopoietic Cell Differentiation and Function Journal of Immunology (Baltimore, Md. : 1950). Jun, 2010 | Pubmed ID: 20483778 Cleavage of Transaldolase by Granzyme B Causes the Loss of Enzymatic Activity with Retention of Antigenicity for Multiple Sclerosis Patients Journal of Immunology (Baltimore, Md. : 1950). Apr, 2010 | Pubmed ID: 20194725 Perforin Activates Clathrin- and Dynamin-dependent Endocytosis, Which is Required for Plasma Membrane Repair and Delivery of Granzyme B for Granzyme-mediated Apoptosis Blood. Feb, 2010 | Pubmed ID: 20038786 Molecular Basis for Antagonism Between PDGF and the TGFbeta Family of Signalling Pathways by Control of MiR-24 Expression The EMBO Journal. Feb, 2010 | Pubmed ID: 20019669 Micromanaging Cancer The New England Journal of Medicine. Oct, 2009 | Pubmed ID: 19812408 MiR-200 Enhances Mouse Breast Cancer Cell Colonization to Form Distant Metastases PloS One. 2009 | Pubmed ID: 19787069 MiR-24 Inhibits Cell Proliferation by Targeting E2F2, MYC, and Other Cell-cycle Genes Via Binding to "seedless" 3'UTR MicroRNA Recognition Elements Molecular Cell. Sep, 2009 | Pubmed ID: 19748357 MiR-34a Contributes to Megakaryocytic Differentiation of K562 Cells Independently of P53 Blood. Sep, 2009 | Pubmed ID: 19584398 Micromanipulating Cancer: MicroRNA-based Therapeutics? RNA Biology. Jul-Aug, 2009 | Pubmed ID: 19535911 Numbers Matter: Quantitative and Dynamic Analysis of the Formation of an Immunological Synapse Using Imaging Flow Cytometry Journal of Immunological Methods. Aug, 2009 | Pubmed ID: 19524586 The Cytotoxic T Lymphocyte Protease Granzyme A Cleaves and Inactivates Poly(adenosine 5'-diphosphate-ribose) Polymerase-1 Blood. Aug, 2009 | Pubmed ID: 19506301 Micromanagers of Immune Cell Fate and Function Advances in Immunology. 2009 | Pubmed ID: 19477322 Harvard's Women Four Years Later Nature Immunology. Jun, 2009 | Pubmed ID: 19448653 MiR-24-mediated Downregulation of H2AX Suppresses DNA Repair in Terminally Differentiated Blood Cells Nature Structural & Molecular Biology. May, 2009 | Pubmed ID: 19377482 The SET Complex Acts As a Barrier to Autointegration of HIV-1 PLoS Pathogens. Mar, 2009 | Pubmed ID: 19266025 Durable Protection from Herpes Simplex Virus-2 Transmission Following Intravaginal Application of SiRNAs Targeting Both a Viral and Host Gene Cell Host & Microbe. Jan, 2009 | Pubmed ID: 19154990 RIG-ing an Antitumor Response Nature Medicine. Nov, 2008 | Pubmed ID: 18989278 Identification and Characterization of PWWP Domain Residues Critical for LEDGF/p75 Chromatin Binding and Human Immunodeficiency Virus Type 1 Infectivity Journal of Virology. Dec, 2008 | Pubmed ID: 18799576 Whither or Wither Microbicides? Science (New York, N.Y.). Jul, 2008 | Pubmed ID: 18653884 A PP4-phosphatase Complex Dephosphorylates Gamma-H2AX Generated During DNA Replication Molecular Cell. Jul, 2008 | Pubmed ID: 18614045 Granzyme A Cleaves a Mitochondrial Complex I Protein to Initiate Caspase-independent Cell Death Cell. May, 2008 | Pubmed ID: 18485875 RNA Interference and Cancer: Endogenous Pathways and Therapeutic Approaches Advances in Experimental Medicine and Biology. 2008 | Pubmed ID: 18437900 P16(INK4a) Translation Suppressed by MiR-24 PloS One. 2008 | Pubmed ID: 18365017 Death by a Thousand Cuts: Granzyme Pathways of Programmed Cell Death Annual Review of Immunology. 2008 | Pubmed ID: 18304003 Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen Science (New York, N.Y.). Feb, 2008 | Pubmed ID: 18187620 Let-7 Regulates Self Renewal and Tumorigenicity of Breast Cancer Cells Cell. Dec, 2007 | Pubmed ID: 18083101 Live Attenuated Listeria Monocytogenes Expressing HIV Gag: Immunogenicity in Rhesus Monkeys Vaccine. Oct, 2007 | Pubmed ID: 17854955 Mutations in the Gene Encoding the 3'-5' DNA Exonuclease TREX1 Are Associated with Systemic Lupus Erythematosus Nature Genetics. Sep, 2007 | Pubmed ID: 17660818 Interfering with Disease: a Progress Report on SiRNA-based Therapeutics Nature Reviews. Drug Discovery. Jun, 2007 | Pubmed ID: 17541417 A Mutation in TREX1 That Impairs Susceptibility to Granzyme A-mediated Cell Death Underlies Familial Chilblain Lupus Journal of Molecular Medicine (Berlin, Germany). May, 2007 | Pubmed ID: 17440703 Delivering the Kiss of Death: Progress on Understanding How Perforin Works Current Opinion in Immunology. Jun, 2007 | Pubmed ID: 17433871 Selective Gene Silencing in Activated Leukocytes by Targeting SiRNAs to the Integrin Lymphocyte Function-associated Antigen-1 Proceedings of the National Academy of Sciences of the United States of America. Mar, 2007 | Pubmed ID: 17360483 NFAT5 Regulates HIV-1 in Primary Monocytes Via a Highly Conserved Long Terminal Repeat Site PLoS Pathogens. Dec, 2006 | Pubmed ID: 17173480 Knocking Down Disease with SiRNAs Cell. Jul, 2006 | Pubmed ID: 16873051 Silencing Viral Infection PLoS Medicine. Jul, 2006 | Pubmed ID: 16848617 Running Interference: Prospects and Obstacles to Using Small Interfering RNAs As Small Molecule Drugs Annual Review of Biomedical Engineering. 2006 | Pubmed ID: 16834561 The Exonuclease TREX1 is in the SET Complex and Acts in Concert with NM23-H1 to Degrade DNA During Granzyme A-mediated Cell Death Molecular Cell. Jul, 2006 | Pubmed ID: 16818237 Determinants of Specific RNA Interference-mediated Silencing of Human Beta-globin Alleles Differing by a Single Nucleotide Polymorphism Proceedings of the National Academy of Sciences of the United States of America. Apr, 2006 | Pubmed ID: 16585504 Granzyme A, Which Causes Single-stranded DNA Damage, Targets the Double-strand Break Repair Protein Ku70 EMBO Reports. Apr, 2006 | Pubmed ID: 16440001 An SiRNA-based Microbicide Protects Mice from Lethal Herpes Simplex Virus 2 Infection Nature. Jan, 2006 | Pubmed ID: 16306938 A Phosphatase Complex That Dephosphorylates GammaH2AX Regulates DNA Damage Checkpoint Recovery Nature. Jan, 2006 | Pubmed ID: 16299494 Your Health in the 21st Century. Silencing Bad Genes Newsweek. 2005 | Pubmed ID: 16375146 Gamma-H2AX Dephosphorylation by Protein Phosphatase 2A Facilitates DNA Double-strand Break Repair Molecular Cell. Dec, 2005 | Pubmed ID: 16310392 Perforin Triggers a Plasma Membrane-repair Response That Facilitates CTL Induction of Apoptosis Immunity. Sep, 2005 | Pubmed ID: 16169498 Listeria-infected Myeloid Dendritic Cells Produce IFN-beta, Priming T Cell Activation Journal of Immunology (Baltimore, Md. : 1950). Jul, 2005 | Pubmed ID: 15972676 Vaccinia Virus Induces Strong Immunoregulatory Cytokine Production in Healthy Human Epidermal Keratinocytes: a Novel Strategy for Immune Evasion Journal of Virology. Jun, 2005 | Pubmed ID: 15919891 Antibody Mediated in Vivo Delivery of Small Interfering RNAs Via Cell-surface Receptors Nature Biotechnology. Jun, 2005 | Pubmed ID: 15908939 Granzyme B Binds to Target Cells Mostly by Charge and Must Be Added at the Same Time As Perforin to Trigger Apoptosis Journal of Immunology (Baltimore, Md. : 1950). May, 2005 | Pubmed ID: 15843543 Lentiviral Delivery of Short Hairpin RNAs Protects CD4 T Cells from Multiple Clades and Primary Isolates of HIV Blood. Aug, 2005 | Pubmed ID: 15831707 Granzyme A Induces Caspase-independent Mitochondrial Damage, a Required First Step for Apoptosis Immunity. Mar, 2005 | Pubmed ID: 15780992 The Prospect of Silencing Disease Using RNA Interference JAMA. Mar, 2005 | Pubmed ID: 15769970 Adenovirus Protein VII Functions Throughout Early Phase and Interacts with Cellular Proteins SET and Pp32 Journal of Virology. Feb, 2005 | Pubmed ID: 15681448 The Silent Revolution: RNA Interference As Basic Biology, Research Tool, and Therapeutic Annual Review of Medicine. 2005 | Pubmed ID: 15660519 Cross-clade CD8 T-cell Responses to HIV(IIIB) and Chinese B' and C/B' Viruses in North American and Chinese HIV-seropositive Donors Journal of Acquired Immune Deficiency Syndromes (1999). Dec, 2004 | Pubmed ID: 15602120 Small Interfering RNA Targeting Fas Protects Mice Against Renal Ischemia-reperfusion Injury Proceedings of the National Academy of Sciences of the United States of America. Oct, 2004 | Pubmed ID: 15466709 Increased Expression of the Natural Killer Cell Inhibitory Receptor CD85j/ILT2 on Antigen-specific Effector CD8 T Cells and Its Impact on CD8 T-cell Function Immunology. Aug, 2004 | Pubmed ID: 15270723 Tracking the Killers: How Should We Measure CD8 T Cells in HIV Infection? AIDS (London, England). Jul, 2004 | Pubmed ID: 15238766 Proliferation Responses to HIVp24 During Antiretroviral Therapy Do Not Reflect Improved Immune Phenotype or Function AIDS (London, England). Mar, 2004 | Pubmed ID: 15090765 Public Health. A Sound Rationale Needed for Phase III HIV-1 Vaccine Trials Science (New York, N.Y.). Jan, 2004 | Pubmed ID: 14726576 Mapping Cross-clade HIV-1 Vaccine Epitopes Using a Bioinformatics Approach Vaccine. Oct, 2003 | Pubmed ID: 14505932 Nuclear War: the Granzyme A-bomb Current Opinion in Immunology. Oct, 2003 | Pubmed ID: 14499264 Interfering with Disease: Opportunities and Roadblocks to Harnessing RNA Interference Trends in Molecular Medicine. Sep, 2003 | Pubmed ID: 13129706 Sustained Small Interfering RNA-mediated Human Immunodeficiency Virus Type 1 Inhibition in Primary Macrophages Journal of Virology. Jul, 2003 | Pubmed ID: 12805416 The ABCs of Granule-mediated Cytotoxicity: New Weapons in the Arsenal Nature Reviews. Immunology. May, 2003 | Pubmed ID: 12766758 A Fragment of Anthrax Lethal Factor Delivers Proteins to the Cytosol Without Requiring Protective Antigen Proceedings of the National Academy of Sciences of the United States of America. May, 2003 | Pubmed ID: 12740437 The Gp49B1 Inhibitory Receptor Regulates the IFN-gamma Responses of T Cells and NK Cells Journal of Immunology (Baltimore, Md. : 1950). Apr, 2003 | Pubmed ID: 12682239 Tumor Suppressor NM23-H1 is a Granzyme A-activated DNase During CTL-mediated Apoptosis, and the Nucleosome Assembly Protein SET is Its Inhibitor Cell. Mar, 2003 | Pubmed ID: 12628186 RNA Interference Targeting Fas Protects Mice from Fulminant Hepatitis Nature Medicine. Mar, 2003 | Pubmed ID: 12579197 Cleaving the Oxidative Repair Protein Ape1 Enhances Cell Death Mediated by Granzyme A Nature Immunology. Feb, 2003 | Pubmed ID: 12524539 HIV-1 Antiviral Activity of Recombinant Natural Killer Cell Enhancing Factors, NKEF-A and NKEF-B, Members of the Peroxiredoxin Family The Journal of Biological Chemistry. Jan, 2003 | Pubmed ID: 12421812 Most Antiviral CD8 T Cells During Chronic Viral Infection Do Not Express High Levels of Perforin and Are Not Directly Cytotoxic Blood. Jan, 2003 | Pubmed ID: 12393740 The Functional CD8 T Cell Response to HIV Becomes Type-specific in Progressive Disease The Journal of Clinical Investigation. Nov, 2002 | Pubmed ID: 12417573 Defying Death--HIV Mutation to Evade Cytotoxic T Lymphocytes The New England Journal of Medicine. Oct, 2002 | Pubmed ID: 12374884 Avoiding the Kiss of Death: How HIV and Other Chronic Viruses Survive Current Opinion in Immunology. Aug, 2002 | Pubmed ID: 12088683 SiRNA-directed Inhibition of HIV-1 Infection Nature Medicine. Jul, 2002 | Pubmed ID: 12042777 Engineered Listeria Monocytogenes As an AIDS Vaccine Vaccine. May, 2002 | Pubmed ID: 11983264 HMG2 Interacts with the Nucleosome Assembly Protein SET and is a Target of the Cytotoxic T-lymphocyte Protease Granzyme A Molecular and Cellular Biology. Apr, 2002 | Pubmed ID: 11909973 A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells Farokh Dotiwala1, Isabelle Fellay2, Luis Filgueira2, Denis Martinvalet3, Judy Lieberman1, Michael Walch2 1Cellular and Molecular Medicine Program, Boston Children’s Hospital and Harvard Medical School, 2Department of Medicine, University of Fribourg, 3Centre Médical Universitaire, University of Geneva JoVE 52911 生物学
A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells Farokh Dotiwala1, Isabelle Fellay2, Luis Filgueira2, Denis Martinvalet3, Judy Lieberman1, Michael Walch2 1Cellular and Molecular Medicine Program, Boston Children’s Hospital and Harvard Medical School, 2Department of Medicine, University of Fribourg, 3Centre Médical Universitaire, University of Geneva JoVE 52911 生物学